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The map label for this gene is metK

Identifier: 15924780

GI number: 15924780

Start: 1921008

End: 1922204

Strand: Reverse

Name: metK

Synonym: SAV1790

Alternate gene names: 15924780

Gene position: 1922204-1921008 (Counterclockwise)

Preceding gene: 15924783

Following gene: 15924779

Centisome position: 66.78

GC content: 37.59

Gene sequence:

>1197_bases
ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAAATCGCTGACCAAGTGTCAGA
TGCAATATTAGATGCTATTTTAAAAGACGACCCAAATGCACGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCAT
TGATCGCCGGCGAAATTTCTACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAACAATCACCTGACATTGCACA
AGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGCGAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTT
TAATGTTTGGTTACGCAACGAATGAAACAGAAACGTACATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
TTATCTGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAGGTCACTGTTGAATATGATGA
AAATGATAACCCAGTACGTATTGATACGATTGTCGTATCAACACAACATGCTGATGATGTGACACTTGAACAAATTCAAG
AAGACATCAAAGCGCATGTCATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATCATTGTTGATACGTATGGTGG
CTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGATCCTACAAAAGTAGACCGCTCAGCTGCATATGCTGCACGTT
ATGTGGCTAAAAATATTGTTGCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGACAACTTGTCGAAGCAGTTAGAAAACACTT
TGACCTAAGACCTGCAGGCATTATTAAAATGCTTGATTTGAAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATT
TTGGACGTACAGATGTATTATTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATATTAA

Upstream 100 bases:

>100_bases
GGACGTATTAGGCCTTCTCTCTATATTAATAGTTAGAAGGTCTTTTTTATTTAGCTCACAGAGAGAGAATTTTCGTAATA
TAAATTTAAAGGAGCAAACT

Downstream 100 bases:

>100_bases
GATGAATTTAATATAGCTAAATAAAAGATTTTCGGTATATAAATGATAATATGTATCGAAAATCTTTAGCTTTTCTATTA
TAATTAAAGTTATTCATATA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY

Sequences:

>Translated_398_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY
>Mature_398_residues
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGY
TRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAE
PVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=58.5301837270341, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=58.005249343832, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=391, Percent_Identity=62.9156010230179, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17538494, Length=388, Percent_Identity=54.639175257732, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=397, Percent_Identity=54.6599496221663, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=54.8969072164949, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=55.0387596899225, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=50.2577319587629, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=53.1157270029674, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=346, Percent_Identity=52.6011560693642, Blast_Score=352, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=339, Percent_Identity=51.3274336283186, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=55.8139534883721, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=382, Percent_Identity=56.5445026178011, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=388, Percent_Identity=55.6701030927835, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=54.5219638242894, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=54.2635658914729, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=54.2635658914729, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52.8, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=56.3492063492063, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_STAA1 (A7X3N2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442365.1
- ProteinModelPortal:   A7X3N2
- SMR:   A7X3N2
- STRING:   A7X3N2
- EnsemblBacteria:   EBSTAT00000005237
- GeneID:   5561489
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1775
- eggNOG:   COG0192
- GeneTree:   EBGT00050000024042
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   SAUR418127:SAHV_1775-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43791; Mature: 43791

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEIS
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCEEEEECCCC
TTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDS
CCEEECCHHHHHHHHHHCCCCHHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCC
EEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQ
HHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
EEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHEEEEECCCCHHHCCCCCEEEECC
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIV
CCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDL
HHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHC
KQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEIS
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCEEEEECCCC
TTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDS
CCEEECCHHHHHHHHHHCCCCHHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCC
EEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQ
HHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEE
VTVEYDENDNPVRIDTIVVSTQHADDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINP
EEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHEEEEECCCCHHHCCCCCEEEECC
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIV
CCCEEEECCCCCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDL
HHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHC
KQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVKY
CCHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA