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The map label for this gene is pfkA

Identifier: 15924688

GI number: 15924688

Start: 1812429

End: 1813352

Strand: Reverse

Name: pfkA

Synonym: SAV1698

Alternate gene names: 15924688

Gene position: 1813352-1812429 (Counterclockwise)

Preceding gene: 15924690

Following gene: 15924687

Centisome position: 63.0

GC content: 37.23

Gene sequence:

>924_bases
ATGAATGCTGCCGTAAGAGCAGTTGTTCGTACAGCAATTTACAATGAAATTGAAGTTTATGGTGTGTATCATGGTTACCA
AGGATTGTTAAATGATGATATTCATAAACTTGAATTAGGATCAGTTGGGGATACGATTCAGCGTGGAGGTACATTCTTGT
ATTCAGCAAGATGTCCAGAGTTTAAGGAGCAAGAAGTACGTAAAGTTGCAATCGAAAACTTACGTAAAAGAGGGATTGAG
GGCCTTGTAGTTATTGGTGGTGACGGTAGTTATCGCGGTGCACAACGCATCAGTGAGGAATGCAAAGAAATTCAAACTAT
CGGTATTCCTGGTACGATTGACAATGATATCAATGGTACTGATTTTACAATTGGATTTGACACAGCTTTAAATACGATTA
TTGGCTTAGTCGACAAAATTAGAGATACTGCGTCAAGTCACGCACGAACATTTATCATTGAAGCAATGGGCCGTGATTGT
GGAGATCTAGCATTATGGGCTGGATTATCAGTTGGTGCTGAGACAATTGTAGTTCCAGAAGTGAAAACAGATATTAAAGA
AATAGCTGATAAAATTGAACAAGGTATTAAACGTGGTAAGAAACACTCAATCGTTCTTGTAGCAGAAGGTTGCATGACTG
CGCAAGATTGTCAAAAAGAATTATCACAATACATCAATGTTGATAATAGAGTGTCTGTGTTAGGTCACGTTCAACGTGGT
GGTAGCCCAACAGGTGCGGATAGAGTTTTAGCATCACGTTTAGGTGGATATGCGGTAGACTTATTAATGCAAGGTGAAAC
AGCTAAGGGTGTTGGAATTAAGAACAATAAAATTGTAGCAACATCTTTTGATGAAATTTTTGATGGTAAAGATCATAAAT
TTGATTATAGTCTATATGAACTTGCTAACAAGTTATCTATATAA

Upstream 100 bases:

>100_bases
CAATTTGTGGTAATTTAGAGTTAAAGATAAATCAGTTATAGAAAGGTATGTCGTCATGAAGAAAATTGCAGTTTTAACTA
GTGGTGGAGATTCACCTGGA

Downstream 100 bases:

>100_bases
GATTTCAGGAGGAATTATAAAATGAGAAAAACTAAAATTGTATGTACAATTGGACCAGCTTCAGAATCAGAAGAAATGAT
TGAGAAATTAATCAATGCTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIE
GLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDC
GDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRG
GSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

Sequences:

>Translated_307_residues
MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIE
GLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDC
GDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRG
GSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI
>Mature_307_residues
MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIE
GLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDC
GDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRG
GSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453619, Length=298, Percent_Identity=42.2818791946309, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI266453768, Length=298, Percent_Identity=42.2818791946309, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI266453748, Length=298, Percent_Identity=42.2818791946309, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI4505749, Length=298, Percent_Identity=42.2818791946309, Blast_Score=214, Evalue=6e-56,
Organism=Homo sapiens, GI11321601, Length=298, Percent_Identity=41.6107382550336, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI48762920, Length=297, Percent_Identity=39.3939393939394, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI1790350, Length=305, Percent_Identity=48.8524590163934, Blast_Score=294, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI25147584, Length=297, Percent_Identity=41.4141414141414, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=33.974358974359, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI72003601, Length=336, Percent_Identity=31.25, Blast_Score=148, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=316, Percent_Identity=38.9240506329114, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323861, Length=317, Percent_Identity=36.2776025236593, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI17647809, Length=298, Percent_Identity=43.2885906040268, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24652337, Length=298, Percent_Identity=43.2885906040268, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI28573326, Length=298, Percent_Identity=43.2885906040268, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_STAA2 (A6U2G5)

Other databases:

- EMBL:   CP000736
- RefSeq:   YP_001316920.1
- ProteinModelPortal:   A6U2G5
- SMR:   A6U2G5
- STRING:   A6U2G5
- EnsemblBacteria:   EBSTAT00000015063
- GeneID:   5315767
- GenomeReviews:   CP000736_GR
- KEGG:   sah:SaurJH1_1789
- eggNOG:   COG0205
- GeneTree:   EBGT00050000024765
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- ProtClustDB:   PRK03202
- BioCyc:   SAUR359787:SAURJH1_1789-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 33397; Mature: 33397

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 129-129 BINDING 164-164 BINDING 245-245 BINDING 251-251 BINDING 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPE
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHCCCEEEECCCCC
FKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPE
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECH
VKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRG
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYE
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHH
LANKLSI
HHHHCCC
>Mature Secondary Structure
MNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDTIQRGGTFLYSARCPE
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHCCCEEEECCCCC
FKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQTIGIPGTIDNDINGT
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
DFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPE
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECH
VKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRG
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
GSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYE
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHH
LANKLSI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA