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The map label for this gene is mnhD

Identifier: 15923939

GI number: 15923939

Start: 989758

End: 991254

Strand: Reverse

Name: mnhD

Synonym: SAV0949

Alternate gene names: 15923939

Gene position: 991254-989758 (Counterclockwise)

Preceding gene: 15923940

Following gene: 15923938

Centisome position: 34.44

GC content: 33.73

Gene sequence:

>1497_bases
ATGATTGAAAGTAATATGCTTGTGTTAACGCTAGTTATTCCAGTAATTACAGCCATATTACTTGTCTTTATTGGAAAGCG
TCCTATTATAAAGCGTTATGTCGCACTAGGCGGTACATTATTAACCTTGGTCGCGGCAATTATCAATTTAGCAAATGTTG
TTAAACATGGACCAATACGCGTAGAGCTAGGTTCATGGAAAGCACCATACAGTATTGTCTTTGTATTAGACATATTCAGT
GCCCTACTAATAATTACTAGTATTATTATTACAGCTATTGTGATTTTATATTCATACCAAACAATTGGTATTGAACGTGA
AAGATATTATTATTATTTTTCAGTACTATTTATGTTAATAGGTATCATTGGTGCATTCACAACTGGTGACATTTTCAACC
TATTCGTATTTTTCGAAGTATTTTTAATGTCATCTTATTTCTTATTAGTAATTGGTTCTACAAAAATTCAACTACAAGAA
ACAATCAAATATGTTCTTGTTAACGTTGTATCGTCATCCTTCTTCGTAATGGGTGTAGCGATTTTATATTCAGTAGTAGG
AACATTGAATCTAGCAGATATAAGCAATAAATTAGCTAACCTTTCAGCGCATGACAGTGGCTTAGTCAATATCGTTTTCA
TACTATTTATCTTTGTTTTTGCTACAAAAGCTGGCGTATTCCCAATGTTTGTTTGGTTACCTAGTGCATACTATGCACCA
CCAATTCCAATCATTGCATTCTTTGGTGCTTTGTTAACAAAAGTTGGTGTTTATGCAATCGCTAGAACATTAAGCCTATT
CTTTAGTGATAATGTAAGCTTTTCACATTATGTCATTTTATTCTTAGCACTATTAACGATCATCTTTGGTTGTGTCGGTG
CTGTTGCATATGCCAACATTAAAAAAATCATCCTATACAATGTCATGATTGCTGTAGGTGTGATCTTAGTCGGCGTTGCA
ATGATGACAGAGTCAGGTATGATTGGGGCTATTTACTATACATTGCACGACATGTTAGTAAAACTTGCATTATTCTTGCT
TATCGGAATTATGATTAAAATTACAGGCACTGCTGATTTAAGACAATTTGGTGGCTTAATTAAACGATATCCTGTACTTG
GTTGGTCATTCTTTATCGCAGCATTAAGTTTAGCTGGCATTCCCCCACTCAGTGGCTTTTATGGAAAATTTTTCATTGTC
CAATCAACATTTGAAAGAGGATTTTATTTAAGTGGTGTCATCGTCTTATTATCAAGTTTAGTCGTATTATATTCTGTCAT
TCGCATATTCCTACAAGGCTTTTTCGGTCAACCAAAAGGATATGATCTTAATAACAAAGTCGACGTCAAATATTTAACTA
CGATTGCAATCGTTGCAGTTGTGATAACTGTTTTATATGGATTGTCAGCAGATTACTTATATCCAATGGTAAAAGCTGGT
GCAGAAACATTCTACAATCCATCAACCTACGTTAAAGCTGTATTAGGAGGTAAATAA

Upstream 100 bases:

>100_bases
ATCGCCTTTGCGACAACAGCCTTTTTCTTAGTATTAGCATTTAGAACATATAAAGAATTAGGCACAGATAACGTTGAGAG
TATGAAAGGAGTTCCAGAAG

Downstream 100 bases:

>100_bases
CATGGCTGTACAATTAGTATTAAACTTTATTATCGCAGTGTTTTGGTTGTTTGTAACAAATAGTTACACAACAAATAACT
TTGTGTTAGGTTTCATCTTT

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mnh complex subunit D1

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK

Sequences:

>Translated_498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK
>Mature_498_residues
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFS
ALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVA
MMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIV
QSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
AETFYNPSTYVKAVLGGK

Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family

Homologues:

Organism=Homo sapiens, GI251831117, Length=387, Percent_Identity=25.8397932816537, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788827, Length=391, Percent_Identity=26.8542199488491, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1788613, Length=352, Percent_Identity=26.9886363636364, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788831, Length=394, Percent_Identity=25.1269035532995, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI2367154, Length=331, Percent_Identity=25.3776435045317, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788829, Length=437, Percent_Identity=23.5697940503432, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI145693160, Length=381, Percent_Identity=25.1968503937008, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788614, Length=419, Percent_Identity=23.1503579952267, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNHD1_STAA1 (A7X0F9)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441534.1
- STRING:   A7X0F9
- EnsemblBacteria:   EBSTAT00000002987
- GeneID:   5560307
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0944
- eggNOG:   COG0651
- GeneTree:   EBGT00050000024644
- HOGENOM:   HBG643593
- OMA:   THTILAW
- ProtClustDB:   PRK07691
- BioCyc:   SAUR418127:SAHV_0944-MONOMER
- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437
- TIGRFAMs:   TIGR00944

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54757; Mature: 54757

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd441ac8)-; HASH(0xdcd2a7c)-; HASH(0xdc997bc)-; HASH(0xd4212ac)-; HASH(0xc88d5c0)-; HASH(0xd910750)-; HASH(0xdcb34f4)-; HASH(0xd6aa790)-; HASH(0xcc8b4c0)-; HASH(0xe0a5300)-; HASH(0xdc0b150)-; HASH(0xbd63804)-; HASH(0xc9bd5f0)-; HASH(0xdbc5c20)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEE
VELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHHHCCHHHHHHHHHHHHH
VVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
AETFYNPSTYVKAVLGGK
HHHCCCCHHHHHHHHCCH
>Mature Secondary Structure
MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLVAAIINLANVVKHGPIR
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEE
VELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTIGIERERYYYYFSVLFMLI
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQETIKYVLVNVVSSSFFVMGVA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFATKAGVFPMFVWLPSAYYAP
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCC
PIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVILFLALLTIIFGCVGAVAYANI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDMLVKLALFLLIGIMIKITGTADL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGKFFIVQSTFERGFYLSGVIVLLSSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEHHHHCCHHHHHHHHHHHHH
VVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTTIAIVAVVITVLYGLSADYLYPMVKAG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
AETFYNPSTYVKAVLGGK
HHHCCCCHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA