Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 15923403

GI number: 15923403

Start: 456120

End: 457913

Strand: Reverse

Name: pcrA [H]

Synonym: SAV0413

Alternate gene names: 15923403

Gene position: 457913-456120 (Counterclockwise)

Preceding gene: 15923404

Following gene: 15923402

Centisome position: 15.91

GC content: 29.04

Gene sequence:

>1794_bases
ATGACAAATATCTTCGAAAAAATCGTAAATGATTTAAATTTAGAACAAAAAGATGTTGTGGAAAGTGAAGATAACCTTTA
TGTAAATGCATGTCCTGGGAGTGGGAAAACAAGGGTACTTACAAGGAAAATTGCTTACCAAGCAATTAAACACGATGGAT
CACTAAAAAGAATAATTGCTATCACATACACTAATAGAGCTGCTGAAGAAATTAAGGAACGCCTAAGTTTACTTAGTATA
GATGATGATATTAATATTTGGGTGGGGACAATACATCAGTTTTGTCTTGAATTTATTATTTATCCTTTTAAAATGAATCT
CTCAAGAGTATCAAAAGGATTTACAATTATTGATGATTATACTCAAAGAGCTTACGTCAGCCAAATTAATAATATGCTGA
ATTTAAATCTCAAAACGTATGAACTGAACAAAATTGATTTGTCACTTTCTCATGATTTTAAAATTAATGAAGTTCAGTAT
ACTCAAGTTTCTGAAATGTATCACACTATGTTGGAAAAAAATAAAGAAATTGATTTTGATATGATTTTACTAGTAGCGTA
TAAAATTTTAACTGTAAACAGTAACGTTGCACGTAATATTGCTAGTAATATAAGGGCGATTTATGTGGATGAATTTCAAG
ATACTAGAGAATTACAATACAATGTAATAGCTAAATTACTTCAAAACAACCCTCAAATTCAGTCAATGTTTGTTGGAGAT
ATTGACCAAGCAATTTATGGATCATTAGATGGTATAGCTAAATCAGTTGAAGAACTAGAAATGCTAACTAGTCACACTTT
TCAATCAAAGACGCTACATGGCTGTTACAGGTCTAATCAGAGATTAGTAGACTTCTACTCTAATTTTCAGAGTTGTCCTT
ACGAGATTGAAAGCAGAGGGAATAATAAAAACGACCGTGGTTTTATAAGTTATGATTGCAAAGTTACTAAGGAGGATTTA
CCCAACATAATAAAAAATATAATAAAGGAAAAGATTGAAAGCGGCATTCATGAAAAAGATATTTGTGTACTTGCTCCTCA
ACACTATCCTCTGTTTTCACTGGGTGAGTATTTAAAGAAAGAATTACCGTATTGCAATTTTGATGCTATAAATATATCAC
CAATTAAGGTAAATAATCATAGTTTATTTTTCAAGTTAGCAATTTTGTACTTTACTGAATCAGGTGAAAAGGTTCGAAGA
AGAAAGCTAATTGCAAACGATATTTTAATTATATTAAAAAATGAATTTGGAATAGAAGTTAAAGATTATTTTATTGACTT
AGATTTATTACAGCTAATAAACAGTGTGAAAAGAATGTATAGTGGAGACGACAACGGTGTTGATTTATACAAATTTGTTA
CCAAACATATATTTGTTAATCTTAATATTGACGAGAAAAATTACCCGTCTATTTTTAAGCACTATCTATTTTTTATTAAC
GAAGTAGAAGATCGGATAAAAAATAATTCATTATCTAGTAGTGTATCTAGTTTTAAGAAGATGTTTAAATCTCGAGATGG
TATCACACTTACTACAGCACATAAAGTTAAAGGAAATGAGTATGATACTGTTATCGCTATTAATCTTCTAAACGGGAAAA
TTCCTCATTGGAATGAAATTTTTAATGTTTCTGATAAAGGAATTAGTTCAGCGAGAAAACTACTATACGTAATTTGTTCA
AGAGCAAAAAATAATTTATTTCTTTTCTCAGAGGTGGGTTATTTTACGAGTAAAGGAGACTTGCTTTATCCCACTCAATT
ATTAGATAAAATAGAATTTGATTATAATATTTAA

Upstream 100 bases:

>100_bases
TAATTTCTTTATTAGAGTACTTTGGTAAAGATGAGGATGTTGTGTATCTAATCAATCAGATAGAAAATTACACAAAAATA
CTTTATGCTGAGGTTTCAAA

Downstream 100 bases:

>100_bases
AATACCTAGGTAGTCGTTGTTTTAAATATAGTAGGAATCGGACGCTCAGTACGGAGCGTCTATTTTTTTACCCAATTTTG
AAAGGAAGTGAACTTATGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSI
DDDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGD
IDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDL
PNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRR
RKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFIN
EVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICS
RAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI

Sequences:

>Translated_597_residues
MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSI
DDDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGD
IDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDL
PNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRR
RKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFIN
EVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICS
RAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI
>Mature_596_residues
TNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSID
DDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQYT
QVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDI
DQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLP
NIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRR
KLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINE
VEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSR
AKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994965, Length=250, Percent_Identity=28.4, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322369, Length=318, Percent_Identity=28.9308176100629, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 69133; Mature: 69002

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIA
CCHHHHHHHHHCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
ITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDY
EEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEHH
TQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTMLEKNKEIDFD
HHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCEEECEEEHHHHHHHHHHHHHCCCCCCHH
MILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGD
HHHHHHHHHHEECCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCC
IDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRG
HHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHCCC
NNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKK
CCCCCCCEEEEECEECHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHH
ELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILIILKNEFGIEV
HCCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCEE
KDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFIN
EHHEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHH
EVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEI
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHH
FNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCEECHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIA
CHHHHHHHHHCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
ITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDY
EEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEHH
TQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTMLEKNKEIDFD
HHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCEEECEEEHHHHHHHHHHHHHCCCCCCHH
MILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGD
HHHHHHHHHHEECCHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCC
IDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRG
HHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHCCC
NNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKK
CCCCCCCEEEEECEECHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHH
ELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILIILKNEFGIEV
HCCCCCCCEEEEEEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCEE
KDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFIN
EHHEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHH
EVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEI
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHH
FNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCEECHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA