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The map label for this gene is cbiH [H]

Identifier: 15899068

GI number: 15899068

Start: 2109632

End: 2110384

Strand: Reverse

Name: cbiH [H]

Synonym: SSO2306

Alternate gene names: 15899068

Gene position: 2110384-2109632 (Counterclockwise)

Preceding gene: 15899069

Following gene: 15899067

Centisome position: 70.53

GC content: 35.86

Gene sequence:

>753_bases
ATGAAAATGGGGAGGTTATATATTGTTGGAATCGGACCAGGATCAAAAGAACAAAGGACAATTAAGGCGCAAGAAGTTCT
GGAAAAGTCCAATGTAATAATTGGATATAATACATACTTAAGGTTAATATCGGATGTCCTAGATGGCAAAAAGGAAGTAA
TAGGGGCTAGAATGAAAGAGGAAATATTTAGGGCAAATACTGCAATAGAAAAGGCCTTAGAGTCCAATAATACAGTAGCC
TTAGTATCTAGTGGTGACCCCCAGGTCTACGGAATGGCGGGTTTAGTTTACGACTTGATTGCAAGGAGAAATTTAGATTT
AGACGTCGAAGTGGTACCAGGTGTAACTGCTGCATTAGCAGCCGCTGCGAGATTAGGAAGTCCCCTTTCTCTTGACTTTG
TAGTAATAAGCTTAAGCGATTTACTAATCCCACGAGAGGAGATTTTACATAAAGTAACTAAGGCTGCTGAAGCTGACTTT
GTAATAGTATTTTATAATTTAATTAATGAGAATTTACTAATAGAGGTTATGGATATAATTTCAAAACATAGAAAGCCAAA
TACCCCAGTGGGACTAGTCAAGAGCGCGTATAGGAATAATGAGAACATAATAATTACGACTTTATCGTCATGGAAAGAAT
ATATGAATGAAATAAGTATGAGTACTACAATGATAATAGGAAACTCATTGACATATAGATATAAAAATTACATGATAACA
CCAAGAGGTTACGAGAGGAAATATGAGCTATGA

Upstream 100 bases:

>100_bases
TAGAGACTAAAATTCCATCTGTAGTATTAACTGGAACTTATGGTGGGAGTGGAATAGCAGTGGCGATTATTAATGAGATT
ATCAAAATGGCAATGGAGGG

Downstream 100 bases:

>100_bases
TAATAAACTCTTTGAAGAGATTTGGAATAACCACTGGAGCGGCAGCCTCTGCAGCCGCAAAGGCAGCAGTTATAGGTCTA
CTTAATAAGGAGAAAAGAAG

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKEEIFRANTAIEKALESNNTVA
LVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALAAAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADF
VIVFYNLINENLLIEVMDIISKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMIT
PRGYERKYEL

Sequences:

>Translated_250_residues
MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKEEIFRANTAIEKALESNNTVA
LVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALAAAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADF
VIVFYNLINENLLIEVMDIISKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMIT
PRGYERKYEL
>Mature_250_residues
MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKEEIFRANTAIEKALESNNTVA
LVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALAAAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADF
VIVFYNLINENLLIEVMDIISKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMIT
PRGYERKYEL

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=253, Percent_Identity=24.901185770751, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 27981; Mature: 27981

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKE
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHH
EIFRANTAIEKALESNNTVALVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALA
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEECCCHHHHHH
AAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADFVIVFYNLINENLLIEVMDII
HHHHCCCCCCHHEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHH
SKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMIT
HHCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEEC
PRGYERKYEL
CCCCCCCCCC
>Mature Secondary Structure
MKMGRLYIVGIGPGSKEQRTIKAQEVLEKSNVIIGYNTYLRLISDVLDGKKEVIGARMKE
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHH
EIFRANTAIEKALESNNTVALVSSGDPQVYGMAGLVYDLIARRNLDLDVEVVPGVTAALA
HHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEECCCHHHHHH
AAARLGSPLSLDFVVISLSDLLIPREEILHKVTKAAEADFVIVFYNLINENLLIEVMDII
HHHHCCCCCCHHEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHH
SKHRKPNTPVGLVKSAYRNNENIIITTLSSWKEYMNEISMSTTMIIGNSLTYRYKNYMIT
HHCCCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEEC
PRGYERKYEL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]