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The map label for this gene is rad50

Identifier: 15899022

GI number: 15899022

Start: 2063403

End: 2065997

Strand: Reverse

Name: rad50

Synonym: SSO2249

Alternate gene names: 15899022

Gene position: 2065997-2063403 (Counterclockwise)

Preceding gene: 15899023

Following gene: 15899021

Centisome position: 69.05

GC content: 31.6

Gene sequence:

>2595_bases
ATGAGAATTGATAAAATTACTTTAACTAATTTCTTAAGTCATGAACACAGCGAAATCCAGTTCATGGGTGAAATAAATGT
AATAGTAGGCCAAAATGGAGCAGGTAAAAGCTCTATAATAGACGGTATAGTATTCAGCTTGTTTAGAACCCATAGTAGGG
GAAATAACGACAACTTAATAAGAAAAGGTTCAAATAGAGGTAGTGTTACCTTATACCTTTCCAATGAAAAAGATAAAATA
GAAATAATAAGGGATATAAGGAGCACTACAGAAGATAGGTTAATAAGGAATCAATTCCCAATCGCACGTAGCGCAACAGT
AGTCTCTAATGAAATAGAAAAAATTCTAGGTATTGATAAAGATATTGCCCTATCAACAATAATAGTTAGGCAAGGAGAAC
TGGATAAAATTTTGGAGAATTTCCAAGAAATTATGGGCAAAATTCTTAAATTGGAACTAATTGAGAAACTCATTGATAGT
AGAGGACCAATAGTAGAATTTAGGAAAAACTTGGAGAATAAATTAAGGGAACTAGATAGAATAGAACAAGACTATAACAA
TTTTAAGAAGACCGTGGAGGAAAAAAGAGCTAGAGTATTGGAGTTAAAAAAAGATAAGGAAAAGCTGGAAGATGAGATAA
AGAACCTAGAAAAACGGATAAAAGACATAAAGGATCAATTCGATGAGTATGAGAAAAAAAGAAACCAGTACTTAAAGTTG
ACAACTACCTTAAAGATAAAAGAAGGCGAATTAAATGAGTTAAATAGGAGTATAGAAGAATTGAGGAAACAAACAGAGAA
CATGGATCAACTCGAAAAGGAGATAAACGAACTTGAGAATCTAAGGAACATCAAGCTAAAATTCGAGAAATATGAAGTTT
TAGCGAAAAGTCATACAGAAATGTCAGCCAATGTAATTAATTTAGAAAAAGAAATTGAGGAATACGAAAAAGCTATTAGA
AGAAAGGAAGAACTTGAGCCAAAATACTTAAAATACAAGGAATTAGAAAGGAAACTGGAAGAATTACAGCCAAAATATCA
ACAGTACCTCAAATTGAAATCCGATTTGGATTCTAAACTAAATTTAAAAGAACGTTTAGAGAAAGATGCAAGTGAGCTTT
CTAATGATATAGACAAGGTTAATAGTCTAGAACAAAAGGTAGAGGAGACTAGGAAAAAACAACTTAATCTTAGAGCTCAA
TTAGCCAAAGTTGAAAGCTTAATATCTGAGAAAAATGAGATAATAAATAATATTAGCCAAGTTGAGGGGGAAACTTGTCC
TGTATGTGGAAGACCCCTAGATGAGGAGCACAAACAAAAAATAATAAAAGAAGCAAAAAGTTACATTTTACAATTAGAAT
TAAATAAGAATGAACTAGAAGAAGAACTGAAGAAGATTACTAACGAATTAAATAAAATAGAAAGGGAATATAGAAGACTT
TCAAATAATAAGGCAAGTTACGATAATGTAATGAGACAGCTTAAAAAGCTAAATGAGGAAATAGAAAATCTTCATAGTGA
GATCGAATCTTTAAAGAATATCGACGAGGAAATTAAGAAAATAAATGAAGAGGTAAAAGAACTAAAACTGTATTATGAAG
AATTCATGAGGCTATCTAAGTATACCAAGGAGGAACTTGATAAGAAAAGAGTAAAGTTAGATGAAATGAAAAAGAAAAAA
GAAGAGATTGAAAAAGAAATGAGAGGACTAGAAAGCGAATTAAAAGGATTAGACAGAAAAGCTCTAGAGAGTAAAATTCT
AGATTTAGAGAACAAAAGAGTAAAGTTAGATGAAATGAAAAAGAAAAAAGGAATACTAGAAGACTATATACGTCAAGTTA
AACTATTACAAGAAGAGGTTAAGAATTTAAGGGAGGAAGTAAATATTATACAATTTGATGAAAATAGATATAATGAACTT
AAAACCAGTTTAGATGCTTATAATCTTAGTCTTAAGGAAAAGGAAAATAGAAAGAGTAGAATCGAAGGCGAATTGGAAAG
TTTAGAGAAAGATATAGAGGAGATAAGTAATAGAATTGCGAATTATGAATTACAGCTAAAGGATAGAGAAAAGATAATCA
ATGCTATTAATAAGTTAGAGAAAATTAGATCAGCATTAGGAGAACGCAAATTACAGAGTTATATAATCATGACAACAAAA
CAATTAATCGAAAATAATCTAAACGATATAATATCAAAATTTGATCTATCCATTAAAAACGTTGAGATGGAGATCATGCC
GAAAACTGGCAGAGGAAGGAGTAGTAGTGGGGATATACTAGTATATACGAATAGTGGCGATACCTTACCCATAGTATCAC
TTAGCGGCGGAGAGAGAATTGCCTTATCTATAGCTTTAAGGTTAGCGATAGCAAAAGCACTAATGAGCAATACTAACTTC
TTCATTCTTGACGAGCCAACAATCCACTTAGATGATCAAAGAAAAGCGTATTTAATAGAGATAATTAGGGCTGCAAAGGA
GAGTGTGCCTCAGATAATAGTGGTGACTCACGACGAGGAGGTAGTCCAAGCAGCAGATTATGTTATTCGTGTTGAAAAGA
GAGGAAATAAAAGTTTTGTGAGGGAGGAGACTTGA

Upstream 100 bases:

>100_bases
TTAGCGAGGATGAAGCAAATTTGATCTTACAAATGATAAAGAACGTTGAGTCTGACGAGATAGTAAATGAGATATTAAAA
AAGTTAACAGGTGTTAACTT

Downstream 100 bases:

>100_bases
TAAGAAAAATATATGATAAGTTAGTAGAAAGCCATAATGAGATAAAAAATCAGATTTACAATATTGCCAATTATCTTAAG
CAAGAGATACAAGATAAAGT

Product: purine NTPase

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLIRKGSNRGSVTLYLSNEKDKI
EIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDKDIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDS
RGPIVEFRKNLENKLRELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKL
TTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTEMSANVINLEKEIEEYEKAIR
RKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKLNLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQ
LAKVESLISEKNEIINNISQVEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRL
SNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSKYTKEELDKKRVKLDEMKKKK
EEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMKKKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNEL
KTSLDAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTK
QLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERIALSIALRLAIAKALMSNTNF
FILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEEVVQAADYVIRVEKRGNKSFVREET

Sequences:

>Translated_864_residues
MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLIRKGSNRGSVTLYLSNEKDKI
EIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDKDIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDS
RGPIVEFRKNLENKLRELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKL
TTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTEMSANVINLEKEIEEYEKAIR
RKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKLNLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQ
LAKVESLISEKNEIINNISQVEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRL
SNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSKYTKEELDKKRVKLDEMKKKK
EEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMKKKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNEL
KTSLDAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTK
QLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERIALSIALRLAIAKALMSNTNF
FILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEEVVQAADYVIRVEKRGNKSFVREET
>Mature_864_residues
MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLIRKGSNRGSVTLYLSNEKDKI
EIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDKDIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDS
RGPIVEFRKNLENKLRELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKL
TTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTEMSANVINLEKEIEEYEKAIR
RKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKLNLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQ
LAKVESLISEKNEIINNISQVEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRL
SNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSKYTKEELDKKRVKLDEMKKKK
EEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMKKKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNEL
KTSLDAYNLSLKEKENRKSRIEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTK
QLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERIALSIALRLAIAKALMSNTNF
FILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEEVVQAADYVIRVEKRGNKSFVREET

Specific function: Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of mre11 by unwinding and

COG id: COG0419

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 zinc-hook domain

Homologues:

Organism=Homo sapiens, GI119466532, Length=434, Percent_Identity=22.3502304147465, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI28559088, Length=434, Percent_Identity=22.3502304147465, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RAD50_SULSO (Q97WH0)

Other databases:

- EMBL:   AE006641
- PIR:   B90395
- RefSeq:   NP_343627.1
- ProteinModelPortal:   Q97WH0
- GeneID:   1453744
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2249
- NMPDR:   fig|273057.1.peg.2050
- HOGENOM:   HBG705863
- OMA:   SGGEQIA
- ProtClustDB:   CLSK803275
- BioCyc:   SSOL273057:SSO2249-MONOMER
- GO:   GO:0005694
- HAMAP:   MF_00449
- InterPro:   IPR022982
- InterPro:   IPR007517
- InterPro:   IPR003395
- InterPro:   IPR013134

Pfam domain/function: PF04423 Rad50_zn_hook; PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 101603; Mature: 101603

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS51131 ZN_HOOK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLI
CCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
RKGSNRGSVTLYLSNEKDKIEIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDK
ECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
DIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDSRGPIVEFRKNLENKLRELDR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
TTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSANVINLEKEIEEYEKAIRRKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
NLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQLAKVESLISEKNEIINNISQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
SNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTKEELDKKRVKLDEMKKKKEEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
KKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNELKTSLDAYNLSLKEKENRKSR
HHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHH
IEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTK
HHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERI
HHHHCCHHHHHHHHCCHHCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHH
ALSIALRLAIAKALMSNTNFFILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEE
HHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHH
VVQAADYVIRVEKRGNKSFVREET
HHHHHHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRIDKITLTNFLSHEHSEIQFMGEINVIVGQNGAGKSSIIDGIVFSLFRTHSRGNNDNLI
CCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
RKGSNRGSVTLYLSNEKDKIEIIRDIRSTTEDRLIRNQFPIARSATVVSNEIEKILGIDK
ECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
DIALSTIIVRQGELDKILENFQEIMGKILKLELIEKLIDSRGPIVEFRKNLENKLRELDR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEYEKKRNQYLKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
TTTLKIKEGELNELNRSIEELRKQTENMDQLEKEINELENLRNIKLKFEKYEVLAKSHTE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSANVINLEKEIEEYEKAIRRKEELEPKYLKYKELERKLEELQPKYQQYLKLKSDLDSKL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
NLKERLEKDASELSNDIDKVNSLEQKVEETRKKQLNLRAQLAKVESLISEKNEIINNISQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEGETCPVCGRPLDEEHKQKIIKEAKSYILQLELNKNELEEELKKITNELNKIEREYRRL
HCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
SNNKASYDNVMRQLKKLNEEIENLHSEIESLKNIDEEIKKINEEVKELKLYYEEFMRLSK
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTKEELDKKRVKLDEMKKKKEEIEKEMRGLESELKGLDRKALESKILDLENKRVKLDEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHH
KKKGILEDYIRQVKLLQEEVKNLREEVNIIQFDENRYNELKTSLDAYNLSLKEKENRKSR
HHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHH
IEGELESLEKDIEEISNRIANYELQLKDREKIINAINKLEKIRSALGERKLQSYIIMTTK
HHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLIENNLNDIISKFDLSIKNVEMEIMPKTGRGRSSSGDILVYTNSGDTLPIVSLSGGERI
HHHHCCHHHHHHHHCCHHCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCCHH
ALSIALRLAIAKALMSNTNFFILDEPTIHLDDQRKAYLIEIIRAAKESVPQIIVVTHDEE
HHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCHH
VVQAADYVIRVEKRGNKSFVREET
HHHHHHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11427726