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The map label for this gene is rfbD-2 [H]

Identifier: 15898580

GI number: 15898580

Start: 1616016

End: 1616639

Strand: Reverse

Name: rfbD-2 [H]

Synonym: SSO1783

Alternate gene names: 15898580

Gene position: 1616639-1616016 (Counterclockwise)

Preceding gene: 15898584

Following gene: 15898579

Centisome position: 54.03

GC content: 35.1

Gene sequence:

>624_bases
ATGACTGACGTAGATAAATGCGAAATAGAAAAAGATAAGGCTTACAAAATTAATTCACAAGCAGTGAAACACATTGTAAG
GGCTGGGAGGGTAGTTGATTCATACGTAATTCACATAAGTACTGATTACGTATTTGATGGAAATAAGGGAAATTATAAAG
AAGATAATATACCTAATCCCATAAACTATTACGGCTTAAGTAAGCTTTTGGGAGAAATATTTGCCTCATCATATGACGAA
AGTTTGATAATTAGAACTTCTGGAGTATTTCGAAACAAAGGATTCCCAATCTACGTCTATAAAACACTAAAAGAAGGTAA
AACAGTGCTTGCCTTTAAAGGCTACTATTCCCCAATCTCCGCGAGAAAACTAGCTCTTGCAATAGATGAGCTTTTAGATT
TGAGAAAGACAGGAATAATCCACGTAGCTGGAGAAAGAATATCCCGTTACGAATTAGCTATAAAGATTAAGGAAAAGTTC
AACCTACCCGGAGAAGTTAAGGAAGTTGATGAGATAAAGAGCTGGATAGCTAAGAGACCCTACGATTCCTCATTAGACAT
TTCTAAGGCTAGGAAATTACTTACAATAGATTTTTACACACTGGATTTAGAAGGGATGATTTAA

Upstream 100 bases:

>100_bases
TTCTGAAATTCAAGGTTACAAATTAGACATCACTGACTTTCCACATCTAGAGGACTTTATAATAAAGAAGAGACCAGATG
TAATAATAAATACTGCTGCA

Downstream 100 bases:

>100_bases
AAATGGAGGCAGTAATCTTACACGGAGGACAAGGAACTCGTTTAAGACCATTAACTCATACTGGTCCTAAGCAATTAATA
AAAGTTGCAGGAAAGCCAAT

Product: dTDP-4-dehydrorhamnose reductase (rfbD-2)

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNPINYYGLSKLLGEIFASSYDE
SLIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPISARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKF
NLPGEVKEVDEIKSWIAKRPYDSSLDISKARKLLTIDFYTLDLEGMI

Sequences:

>Translated_207_residues
MTDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNPINYYGLSKLLGEIFASSYDE
SLIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPISARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKF
NLPGEVKEVDEIKSWIAKRPYDSSLDISKARKLLTIDFYTLDLEGMI
>Mature_206_residues
TDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNPINYYGLSKLLGEIFASSYDES
LIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPISARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKFN
LPGEVKEVDEIKSWIAKRPYDSSLDISKARKLLTIDFYTLDLEGMI

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=209, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 23601; Mature: 23470

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNP
CCCCCCCCCCCCCCEEECHHHHHHHHHHCHHEEEEEEEEEEEEEEECCCCCCCCCCCCCC
INYYGLSKLLGEIFASSYDESLIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPIS
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCEEEEEEECCCCCEEEEEECCCCCCH
ARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKFNLPGEVKEVDEIKSWIAKRP
HHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
YDSSLDISKARKLLTIDFYTLDLEGMI
CCCCCCHHHCCEEEEEEEEEEEECCCC
>Mature Secondary Structure 
TDVDKCEIEKDKAYKINSQAVKHIVRAGRVVDSYVIHISTDYVFDGNKGNYKEDNIPNP
CCCCCCCCCCCCCEEECHHHHHHHHHHCHHEEEEEEEEEEEEEEECCCCCCCCCCCCCC
INYYGLSKLLGEIFASSYDESLIIRTSGVFRNKGFPIYVYKTLKEGKTVLAFKGYYSPIS
HHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCEEEEEEECCCCCEEEEEECCCCCCH
ARKLALAIDELLDLRKTGIIHVAGERISRYELAIKIKEKFNLPGEVKEVDEIKSWIAKRP
HHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCC
YDSSLDISKARKLLTIDFYTLDLEGMI
CCCCCCHHHCCEEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]