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The map label for this gene is rfbB-3 [H]

Identifier: 15898578

GI number: 15898578

Start: 1614044

End: 1614982

Strand: Reverse

Name: rfbB-3 [H]

Synonym: SSO1781

Alternate gene names: 15898578

Gene position: 1614982-1614044 (Counterclockwise)

Preceding gene: 15898579

Following gene: 15898576

Centisome position: 53.97

GC content: 38.23

Gene sequence:

>939_bases
ATGATAATTATAGGCGGTGCTGGTTTCATAGGTTCAGCGTTTGTAAGGGAGGTTAATAAAAGAGGAATTAAACCAATAGT
AGTGGACTTATTAACTTATGCTGGAAGGAAGGAGAACCTCATAGGTACGGAACATGAGTTTATAAGGGCTGATGTGAGAA
GTGAGGAAATTCATGATATAGTTAAGAATTCTGATATAGTTGTAAATTTTGCAGCCGAAACTCACGTTGATAGGTCTATT
TATAGGCCTCAGGATTTCGTAACTACTAATGTGTTAGGAGTAGTGAACTTACTGGAGGCTGCTAGAAAATATGATTTTAA
ATATGTTCATATTTCAACAGATGAGGTTTACGGTGAGGAGTGCGCAGATGAGGATTCGCCTTTACAACCTTCTTCTCCTT
ACAGTGCCTCAAAAGCTTCTGCTGATTTATTCGTTAAGGCTTACGTAAGGACTTACGGAATTAAGGCAATTATTGTAAGA
CCTTCTAATAATTACGGACCTAGACAGTTTCCTGAGAAGCTTATTCCAAAAGTTATCATAAGAACTTTTCTAAACATGCA
CGTTCCAATTTACGGTGATGGTAGAGCTGAGAGAGATTGGATTTACGTTGAGGATACTGTTAGAATAATATATGATATTA
TGGAGAGAAGTGAGTGGAGGGGAGAAGTATACAATATACCCGGAGGGCAAAGGTATAGCGTTCTTGACGTGATAAAGATC
ATAGGGGAGATAATGGGGAAAGAGGTTAAAGTTAAGTTCGTTGACGATAGGCCGGGACATGATAAAAGATATTGCATGAC
TACGAAGCTTAAGTATGAGGTTACACCGTTAAAGGAGGGGTTGAGAAAGACTGTGGAGTGGTATTTGAATAATAGATGGT
GGTGGGAGGATTTAATAAAGGATAAGTTCTTTACTGAGGATGAACCTTGGAAGAAATAA

Upstream 100 bases:

>100_bases
GTGTTTCACTAATGGATTCTCTAATTGGAAATAATTCAACTGTAGAAAAGGGTACTAAATGGCAAAAGTTGATAATAGGT
GAGAACTCATCGGTGATAAT

Downstream 100 bases:

>100_bases
TTTACTTTACATCAAGTGATTGTGTTGAGTAATGATCAGTCTTTTTCTTAGTTAATGTAAAAAGAAAACTGTACTTGCTA
AAATATATCTGTTTACTTCC

Product: dTDP-glucose 4,6-dehydratase (rfbB-3)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDIVKNSDIVVNFAAETHVDRSI
YRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEECADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVR
PSNNYGPRQFPEKLIPKVIIRTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKI
IGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIKDKFFTEDEPWKK

Sequences:

>Translated_312_residues
MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDIVKNSDIVVNFAAETHVDRSI
YRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEECADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVR
PSNNYGPRQFPEKLIPKVIIRTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKI
IGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIKDKFFTEDEPWKK
>Mature_312_residues
MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDIVKNSDIVVNFAAETHVDRSI
YRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEECADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVR
PSNNYGPRQFPEKLIPKVIIRTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKI
IGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIKDKFFTEDEPWKK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=325, Percent_Identity=34.7692307692308, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI42516563, Length=308, Percent_Identity=26.2987012987013, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI56237023, Length=304, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI56118217, Length=304, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI189083684, Length=304, Percent_Identity=27.6315789473684, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=41.8879056047198, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=40.9883720930233, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=25.297619047619, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788365, Length=325, Percent_Identity=24.3076923076923, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788366, Length=201, Percent_Identity=26.865671641791, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=32.6086956521739, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=29.2834890965732, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17539532, Length=309, Percent_Identity=25.8899676375405, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356223, Length=310, Percent_Identity=26.1290322580645, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19923002, Length=300, Percent_Identity=28, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36211; Mature: 36211

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDI
CEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHH
VKNSDIVVNFAAETHVDRSIYRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEE
HCCCCEEEEEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCHH
CADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVRPSNNYGPRQFPEKLIPKVII
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
RTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKI
HHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
IGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIK
HHHHCCCEEEEEEECCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCEEHHHHHH
DKFFTEDEPWKK
HHCCCCCCCCCC
>Mature Secondary Structure
MIIIGGAGFIGSAFVREVNKRGIKPIVVDLLTYAGRKENLIGTEHEFIRADVRSEEIHDI
CEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHH
VKNSDIVVNFAAETHVDRSIYRPQDFVTTNVLGVVNLLEAARKYDFKYVHISTDEVYGEE
HCCCCEEEEEEHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCHH
CADEDSPLQPSSPYSASKASADLFVKAYVRTYGIKAIIVRPSNNYGPRQFPEKLIPKVII
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHH
RTFLNMHVPIYGDGRAERDWIYVEDTVRIIYDIMERSEWRGEVYNIPGGQRYSVLDVIKI
HHHHHCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHH
IGEIMGKEVKVKFVDDRPGHDKRYCMTTKLKYEVTPLKEGLRKTVEWYLNNRWWWEDLIK
HHHHCCCEEEEEEECCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHCCCEEHHHHHH
DKFFTEDEPWKK
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]