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The map label for this gene is pqqE-3 [H]

Identifier: 15898450

GI number: 15898450

Start: 1483225

End: 1484361

Strand: Reverse

Name: pqqE-3 [H]

Synonym: SSO1632

Alternate gene names: 15898450

Gene position: 1484361-1483225 (Counterclockwise)

Preceding gene: 15898451

Following gene: 15898449

Centisome position: 49.61

GC content: 36.68

Gene sequence:

>1137_bases
ATGATTCCAATAAGCGTTCTAGTAACTAATGCTGGTACAGTTTCATTTCACATTAAAGGTGAGTATAATAAGGATAGACC
TAGTAAATTCAGCGACGTATTTAGACCAGTGGTAACGTGGAATCTCACGCGGAAATGCAACTTGAAATGTCTTCATTGTT
ATATTAACGCGTCACCAGAAGGTGAGGATGGGTCAACTACAGAAGAGGCGTTAAGGCTAATAGACGAAATGGCTGAAATG
AGAATTCCATTAATTATAATGAGCGGCGGAGAGCCATTAATGAGAAGGGATTTCTTTGAATTGGCATCATATGCTAGGAT
AAAGGGAATAAAATTAGCCTTATCTACAAATGGCACATTGATTACCGAGAGTGTTGCGAAGAAATTAAAAGAACTGGATT
TCTCTTACGTAGGTATAAGTTTAGATAGTTACGATCCAGAATTTCACGATAGATTTAGAGGTCTAAATGGAGCCTTTAAC
ATGACAGTAAAGGGAATTAAAAATGCAATAAACGTTGGACTAAACGTTGGACTTAGATTTACTATTACGGCTAGAAACAT
TCATCAGATAGACGAATACGTGAAATTGGCCCTGGAACTGGGAGTTAAGAGAATAACGTTTTATCATTTATCAGCTAGTG
GGAGGGGTAAGGAATTAAGGGAGTGGATGTATACTCCAGAAGAATATGTTGGTTTCATAAACAAAATGATTGACTATGCA
ATTAAGCTAAGTGGAAAGATAGAGATTGAGACTACCTTGGGACAATTCGATGGAGTTTACATAGCTAAAAAGTTGGCTAG
AGATGAGGAAGAACTTGAAAAGTACATGAAATTTGTTGAAAATAGTGGAGGATGTGGAAGGAAGATGATATCAATATATC
CAAATGGTGACGTTTATCCTTGTCAATTCATTGACTTTTACAAATTAGGTAACGTTAGGGAAAAACCGCTTAAGGAGATA
ATTAAGAATATTCCCGACCTATTCATAAATACGGACAAGTATTTGAGGGGGGAATATTGTGATAAATGTAAATATAAATC
GGCGTGTAAAGGAGGAGATAGAGCTAGAGCGTATTATTGGAATGGAGATGTTTATGGAGATGACCCATTATGCCCTTTGA
AAACGCTCCACATTTAG

Upstream 100 bases:

>100_bases
AGAGTTAAAAAGATAATTGTGCAAAACAACCCCGTTTAAGAAAATAATTGCTAAAAGATTAGGGATCACTTTTTTATGAC
TAAGATATCTTTTTATTATT

Downstream 100 bases:

>100_bases
TCTTCTGGGAAGTAACTAAAGCTTGCCCATTAACTTGTAAACACTGTAGAGCTAACGCTATAGATAAGCCGTTGCCAGGT
GAGCTTAATACTGAAGAGAG

Product: coenzyme PQQ synthesis protein E (pqqE-3)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGSTTEEALRLIDEMAEM
RIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYWNGDVYGDDPLCPLKTLHI

Sequences:

>Translated_378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGSTTEEALRLIDEMAEM
RIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYWNGDVYGDDPLCPLKTLHI
>Mature_378_residues
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPEGEDGSTTEEALRLIDEMAEM
RIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTLITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFN
MTVKGIKNAINVGLNVGLRFTITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYPCQFIDFYKLGNVREKPLKEI
IKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYWNGDVYGDDPLCPLKTLHI

Specific function: Required for production of the bacteriocin subtilosin. May activate a metalloenzyme that catalyzes a modification of prosubtilosin [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081923, Length=341, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786999, Length=206, Percent_Identity=25.2427184466019, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR008792
- InterPro:   IPR007197 [H]

Pfam domain/function: PF05402 PqqD; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43083; Mature: 43083

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
GEDGSTTEEALRLIDEMAEMRIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCE
ITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFNMTVKGIKNAINVGLNVGLRF
EHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCEECCEEE
TITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYW
EHEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCHHHHCCCHHHCCCCCCEEEEEE
NGDVYGDDPLCPLKTLHI
CCEEECCCCCCCCEECCC
>Mature Secondary Structure
MIPISVLVTNAGTVSFHIKGEYNKDRPSKFSDVFRPVVTWNLTRKCNLKCLHCYINASPE
CCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEEECCCC
GEDGSTTEEALRLIDEMAEMRIPLIIMSGGEPLMRRDFFELASYARIKGIKLALSTNGTL
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCE
ITESVAKKLKELDFSYVGISLDSYDPEFHDRFRGLNGAFNMTVKGIKNAINVGLNVGLRF
EHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHCCEECCEEE
TITARNIHQIDEYVKLALELGVKRITFYHLSASGRGKELREWMYTPEEYVGFINKMIDYA
EEEECHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHH
IKLSGKIEIETTLGQFDGVYIAKKLARDEEELEKYMKFVENSGGCGRKMISIYPNGDVYP
EEECCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCE
CQFIDFYKLGNVREKPLKEIIKNIPDLFINTDKYLRGEYCDKCKYKSACKGGDRARAYYW
EHEEHHHHCCCCCHHHHHHHHHHCCHHEECCCHHHCCCHHHHCCCHHHCCCCCCEEEEEE
NGDVYGDDPLCPLKTLHI
CCEEECCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 10572140; 10809709; 10809710 [H]