Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 15898180

GI number: 15898180

Start: 1175015

End: 1176085

Strand: Direct

Name: proP [H]

Synonym: SSO1338

Alternate gene names: 15898180

Gene position: 1175015-1176085 (Clockwise)

Preceding gene: 15898179

Following gene: 15898182

Centisome position: 39.27

GC content: 35.48

Gene sequence:

>1071_bases
TTGCTTTTTCTATTAACCATAGTCATAGGATATTTTGCCAGACCTTTAGGAGGTATATTCTTTGGACATTATGGAGATAA
GATAGGGAGGAAGAGAATATGGTTTGTTTCACTGCTTGGAATGGGTATTGCTACTACACTAATAGGGTTTTTGCCCACTT
ATAATCAAGTTGGCATATTTGCTACCATACTACTGATTTTGTTACGAATATTGCAAGGCTTTTTCTTAGCTGGAGAATGG
GGAGGAGGAATGACGTTAGTTAATGAGTTTTCTCCAAGTAATTTGAGGGGATTAATGAGTGGTGTACAACAAGGAGGAGC
AGCATTGGGTTTAATATTCGCGGTAATTGCTAGTGAAATAGCCTCGTCTTTAGCACCTGGTTCTGCCTTTGCTACATATG
GTTGGAGAATTATGTTCTGGTTCGGTGTTATTCCACTAATAATAGCTTTAACTGTAAGGTGGAAGGTTGGTGAGAGTATT
GAATGGCTAACTAAGGTAGCAGATAAACCTGAAAAAATTCCTATTGCCACTGTTTTTAAGAAATGGTGGAAGCTGGTTAT
AATAGCTGCTGTAGTGTTGTTTAGTAGTGGGTCTATATACTACGGCATTATAGCGTACATGTCTACATTTTTTGAACTTT
ACACACATTTATCAATATATGAAATAGATAATATTGTATTGGCCACTAATCTAGTATGGTTATTCCTCTCTCCATTGACT
GGTTATTTAAGTGATATATTGAAGATGAGAAAGACATTAATAGCAATACTAGGATTTATCATTGGTATCTTGGTATACCC
AATAATAAATGTGCTTTACACCAGTTCATATATCTTAAGCATTTTAGCCGGTATGCTATTAGGATTTTTATTTGCGTTTC
AATACTCAATATTTCCCGCTTGGTTATCTGAAAACATTAATACTAATGTGAGATACTCGTACATAGCTTTCTCAATCAAT
TTAGGAGTGGCACTTTCATCCTTTGCCCCTTACATAGTAACAGCACTAGGATTAATCTTTCATAATCCGGTCATAGGATT
GCAGTTTTTGATATATCTGCTTCAATATTAG

Upstream 100 bases:

>100_bases
AGGCTGGGGATTTGAACTATTCGATTTAGTAGTCTACCTATATGTTGCAACTACAATATCTCCTCTATTCTTTCCTTCTT
CAAGCAAAATAGCTAGCTTA

Downstream 100 bases:

>100_bases
GAGGAATAGCCGCTTTACTATCACCACCTGATAAAGTGGGAATGCAACTTAGTTGAAAAAAGTCAACTTTTTTCTTCTTC
TCTTTTTCTTAGCTCAAATC

Product: sugar ABC transporter permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIFATILLILLRILQGFFLAGEW
GGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEIASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESI
EWLTKVADKPEKIPIATVFKKWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLT
GYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPAWLSENINTNVRYSYIAFSIN
LGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY

Sequences:

>Translated_356_residues
MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIFATILLILLRILQGFFLAGEW
GGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEIASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESI
EWLTKVADKPEKIPIATVFKKWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLT
GYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPAWLSENINTNVRYSYIAFSIN
LGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY
>Mature_356_residues
MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIFATILLILLRILQGFFLAGEW
GGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEIASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESI
EWLTKVADKPEKIPIATVFKKWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLT
GYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPAWLSENINTNVRYSYIAFSIN
LGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=341, Percent_Identity=29.0322580645161, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788292, Length=342, Percent_Identity=30.4093567251462, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789941, Length=340, Percent_Identity=33.5294117647059, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788942, Length=270, Percent_Identity=27.037037037037, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87082231, Length=172, Percent_Identity=31.3953488372093, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082404, Length=174, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 39558; Mature: 39558

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ATILLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEI
HHHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVFK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHH
KWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSENINTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY
HHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFLLTIVIGYFARPLGGIFFGHYGDKIGRKRIWFVSLLGMGIATTLIGFLPTYNQVGIF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ATILLILLRILQGFFLAGEWGGGMTLVNEFSPSNLRGLMSGVQQGGAALGLIFAVIASEI
HHHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ASSLAPGSAFATYGWRIMFWFGVIPLIIALTVRWKVGESIEWLTKVADKPEKIPIATVFK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHH
KWWKLVIIAAVVLFSSGSIYYGIIAYMSTFFELYTHLSIYEIDNIVLATNLVWLFLSPLT
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYLSDILKMRKTLIAILGFIIGILVYPIINVLYTSSYILSILAGMLLGFLFAFQYSIFPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSENINTNVRYSYIAFSINLGVALSSFAPYIVTALGLIFHNPVIGLQFLIYLLQY
HHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]