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The map label for this gene is pdhD-1 [H]

Identifier: 15897983

GI number: 15897983

Start: 966944

End: 968284

Strand: Reverse

Name: pdhD-1 [H]

Synonym: SSO1123

Alternate gene names: 15897983

Gene position: 968284-966944 (Counterclockwise)

Preceding gene: 15897984

Following gene: 15897982

Centisome position: 32.36

GC content: 35.35

Gene sequence:

>1341_bases
ATGAAATATGATATCGTAATAATTGGCGGTGGTACTGCCGGTTATGTTGCTGGAAGCATATTAGCGAGAAAGGGCAAGAA
GGTACTAGTTGCCGAAAAAGAGAAATTTGGTGGAGTTTGTGTAAACTTCGGCTGTGTACCAAGTATTTTTCTTTTTGATG
CAACCTTTTTATTAAATAGATTTAAAGAAATTGTGTATTATATAGGCTTAGATGGTGAAATTGAATATAAAGATCTCCTT
TTCAGTAAAAGAAACGAAATTATAGACTACTTATCAAACGCCGGAAGAAAGCTAATTGAAGATTCGGGCGGTGAAACTGA
GTTAGGTGAAGTTGAAATAATTTCTCCTAGTACGGTAAAGGTAAATGGGAGAATTGTGGAGTTCGATAATTTAATCATAG
CTACTGGTTCTAAACCGATGGTACCAAGTATTAATGGCATTGAAAATACTTTAAGTGAAGATGATGCGGTTAATTTGAAT
TCAGTACCTTCTTCAATGGTTATTATTGGCGGAGGCTATGCAGGAGTTGAGATAGCTCAAATGTATTCCAGATTAGGGTC
ACAAGTTACCTTATTGTCCAGAAGTAAAATTTTACCAACATTTCCAGAGGATGCTAGAAGTATTATAAAGGATTCCTTGG
AATTCGATGGAGTAAATATAGAGGAAAACATTAGAATAGTGAAGATTCATGATGGAAAAGTGATTACGGAAAAGGGTGAG
GTAGAAGGAAACGTAATAGTGTATGCCACAGGGAGAAGACCGCAATTGCCTAAGGGTATTGAAATACTTGGATTGAGTAT
TAATGAATGTGGGATAGTAGTTGATAAGTATAGAAGAGTAAAGAACAATGTATACGCAATAGGTGATGTAATAGACAAAG
AGAGAAAAACTGCACATTCAGCAATTTTAGATGCAGTAATTGCATCACTACATATCCTTAAGGACGCAACATTTCTTCCC
CTAATAGATAATCTTAAGATACCCCAAGTGTTATATACTGATCCTCAAGTTGGTATCGTAGGTAACGATAAAGAGGCTAA
GGAGTTTTCTGTTTTTCCGTTTGCTGCAACTACCAGAGCAATTATCAATGGATTTAAGGATGGGTACGTTAAACTAGGAA
TAAATGAGAGAAATGAGATAGTTTTTGGAGAAGTAATTGGCGATAAGGCTGAGGAATTAATCAATATTTTAACGTTAGTA
GTAAATAATAGAATAAGAATCGAGAGTTTAGCGCTAATGTCTTTTGTTCACCCTTCATTCTCTGAGGCCATAGTTAATGC
AGCGAAGGGCTTTTTTGATTTAGATGTGGATAAATATAAGAGTAAAGATGGGAAAACTTGA

Upstream 100 bases:

>100_bases
GAGGATGATGTAGTGGAAGGCATAGAACTAGTCGGAGGATCTACATTAATAGATCTGACATTGGAAGCTGATAGGACATT
ATTCTTCTAAATGACCTAGC

Downstream 100 bases:

>100_bases
GGATTATTTAAAGAAGAAGGGATTTTCCCTAGTTAATGAGGGAAAAAGGGAAAGAGTGGTAATGGATGATTATGAGTTTT
TTATTGAGAACTTAACTATA

Product: dihydrolipoamide dehydrogenase (pdhD-1)

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNRFKEIVYYIGLDGEIEYKDLL
FSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVKVNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLN
SVPSSMVIIGGGYAGVEIAQMYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGE
VEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHSAILDAVIASLHILKDATFLP
LIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRAIINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLV
VNNRIRIESLALMSFVHPSFSEAIVNAAKGFFDLDVDKYKSKDGKT

Sequences:

>Translated_446_residues
MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNRFKEIVYYIGLDGEIEYKDLL
FSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVKVNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLN
SVPSSMVIIGGGYAGVEIAQMYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGE
VEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHSAILDAVIASLHILKDATFLP
LIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRAIINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLV
VNNRIRIESLALMSFVHPSFSEAIVNAAKGFFDLDVDKYKSKDGKT
>Mature_446_residues
MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNRFKEIVYYIGLDGEIEYKDLL
FSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVKVNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLN
SVPSSMVIIGGGYAGVEIAQMYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGE
VEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHSAILDAVIASLHILKDATFLP
LIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRAIINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLV
VNNRIRIESLALMSFVHPSFSEAIVNAAKGFFDLDVDKYKSKDGKT

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=464, Percent_Identity=29.5258620689655, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=25, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=24.1685144124169, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=24.1685144124169, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=24.1685144124169, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI148277065, Length=451, Percent_Identity=24.1685144124169, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI148277071, Length=451, Percent_Identity=24.1685144124169, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786307, Length=485, Percent_Identity=28.4536082474227, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=28.159645232816, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789915, Length=348, Percent_Identity=26.7241379310345, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=24.295010845987, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789065, Length=188, Percent_Identity=26.5957446808511, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=28.4501061571125, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=340, Percent_Identity=27.6470588235294, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=340, Percent_Identity=27.6470588235294, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557007, Length=498, Percent_Identity=23.8955823293173, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=389, Percent_Identity=27.2493573264781, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=30.4526748971193, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=26.4957264957265, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6325240, Length=485, Percent_Identity=25.979381443299, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358499, Length=484, Percent_Identity=29.1322314049587, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=24.4210526315789, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=24.4210526315789, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=24.4210526315789, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=22.5872689938398, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48817; Mature: 48817

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNR
CEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHH
FKEIVYYIGLDGEIEYKDLLFSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVK
HHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCEEE
VNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLNSVPSSMVIIGGGYAGVEIAQ
ECCEEEEECCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
MYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGE
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHCEEEEEEECCEEEEECCC
VEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHS
CCCCEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHH
AILDAVIASLHILKDATFLPLIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRA
HHHHHHHHHHHHHCCCCEECHHCCCCCCEEEECCCCEEEECCCCCCCEEEEECCHHHHHH
IINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLVVNNRIRIESLALMSFVHPSF
HHCCCCCCEEEEECCCCCCEEEEHHHCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCH
SEAIVNAAKGFFDLDVDKYKSKDGKT
HHHHHHHHCCCEECCHHHHCCCCCCC
>Mature Secondary Structure
MKYDIVIIGGGTAGYVAGSILARKGKKVLVAEKEKFGGVCVNFGCVPSIFLFDATFLLNR
CEEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHH
FKEIVYYIGLDGEIEYKDLLFSKRNEIIDYLSNAGRKLIEDSGGETELGEVEIISPSTVK
HHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCEEE
VNGRIVEFDNLIIATGSKPMVPSINGIENTLSEDDAVNLNSVPSSMVIIGGGYAGVEIAQ
ECCEEEEECCEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
MYSRLGSQVTLLSRSKILPTFPEDARSIIKDSLEFDGVNIEENIRIVKIHDGKVITEKGE
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHCEEEEEEECCEEEEECCC
VEGNVIVYATGRRPQLPKGIEILGLSINECGIVVDKYRRVKNNVYAIGDVIDKERKTAHS
CCCCEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHHHHH
AILDAVIASLHILKDATFLPLIDNLKIPQVLYTDPQVGIVGNDKEAKEFSVFPFAATTRA
HHHHHHHHHHHHHCCCCEECHHCCCCCCEEEECCCCEEEECCCCCCCEEEEECCHHHHHH
IINGFKDGYVKLGINERNEIVFGEVIGDKAEELINILTLVVNNRIRIESLALMSFVHPSF
HHCCCCCCEEEEECCCCCCEEEEHHHCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCH
SEAIVNAAKGFFDLDVDKYKSKDGKT
HHHHHHHHCCCEECCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]