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The map label for this gene is akH [C]

Identifier: 15897768

GI number: 15897768

Start: 746826

End: 748166

Strand: Reverse

Name: akH [C]

Synonym: SSO0876

Alternate gene names: 15897768

Gene position: 748166-746826 (Counterclockwise)

Preceding gene: 15897769

Following gene: 15897767

Centisome position: 25.0

GC content: 36.54

Gene sequence:

>1341_bases
ATGGCTCTAATAGTTAAAATAGGCGGATCTATACAAAAAGATGAAAAAGATTATGAACTTATCGTAAAGAAGATACAAGA
TTTTTCAAAGAAATCAGATAAGATTATAGTAGTAACATCAGCCATTAAAAACGTTACTAATGAGTTAATAAGCGCAACTT
CGAATACTGATAATTCTCCAAATATTGTGACTGAAATCTACGAGAGACACATAAAACTATTATCTAAGTTAGCAGACGGT
AAGGAATTCGAGAATTCGTTTAAAGATATATCTAGGCTTAGCGATGAATTGTTCAGAGTAGCTTGGTCTATAAGGGTATT
AGACGAAGTGACCCCTAGAGTTAGAGACTACATACTTTCATTTGGAGAGAGAATGGCTACCCTACTCCTTTCAGCAATAT
TAAGAAGTAACGGGATTGAAGCTGAAGGCATAATTACTCCTCCATTTTTAACTGATGAGAATTACGGTGAGGCTAACGTA
ATTGAGGATCTATCTAAAAATGAAATCGCCAACATATTAGAGAATGCGAAAGCTAATGTAATAGTCCTTCCAGGATTTAT
AGGGAGAACTAGAGAGGGAAGATATACTACTTTGGGAAGGGGTGGTAGTGACTATACAGCAACTTTGCTAGGTAAACTAG
TTGGAGTGAGAGAAGTTAGACTAGTTACCGAAGTCCCCGGGATAATGACTGGAGATCCTAAAAAATTCGAAAATGCTAAA
ACGATATCTAGACTCTCGTTGGAAGAGGCTATTGAACTCTCACAGTTAGGAGCTAAAAGGCTGCATCCTAGAACTTTTGA
TCCAGTCTTTGGAAGTGATATGAAGGTAATAGTTGAGTCGCTGTATGAAGACGGATTTACCATAATTAATGGAGAATGCC
AAAATAATGATGGGCTTAAAGGGATTTCACTCCTAGATAATGCGAAATTAATAACAGTTGAGAGTACGAAAATTGTTGGA
AAAATAGGATCTGCTGCAAGAATAACAAATGAAGCAAAGGAAGCTGGAGTTAACATAATATCAATTTCTCAACCAGCTAG
CGAGACTACAATACAACTCGTTGTTGACTCACTTTCTGCGAACAGACTGTTATCGAAATTGGAAGAGATAAGGGGGACTT
TAGTTAAAGATATTGAGGTTAATGATATTAATATCGTAGGTATAGTAGGGTGCGGAATAAAGAAGAAGGAGATCTCTACC
AAGGTTCTTTCAATAGCCTCAAGTTATGATCCGTTAGCGATTTCTAGGGGAATATCTAACGTAAGCATGACCTTTATAGT
TAATAAGGATGAAGGAGAAAAATTAGCAAAAGAGTTGCATAAGGTGATCGTAAGTGGCTGA

Upstream 100 bases:

>100_bases
GGACATTCGTTAAAGGATCCCGATAGTATGACAAAAGCTGAGGCTAAAAGAATATTAGTTAATCCTTTACATATGGAAAA
AATTATCCTAGGTGAGATAA

Downstream 100 bases:

>100_bases
TAAGATAAAAGTTTCGTTACTAGGCTCTACCGGAATGGTAGGGCAAAAAATGGTAAGGATGCTTTCAAAACATCCCTATA
TAGAATTAGTGAAAGTAAGT

Product: aspartokinase (akH)

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSPNIVTEIYERHIKLLSKLADG
KEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANV
IEDLSKNEIANILENAKANVIVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAK
TISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLKGISLLDNAKLITVESTKIVG
KIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEIST
KVLSIASSYDPLAISRGISNVSMTFIVNKDEGEKLAKELHKVIVSG

Sequences:

>Translated_446_residues
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSPNIVTEIYERHIKLLSKLADG
KEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANV
IEDLSKNEIANILENAKANVIVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAK
TISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLKGISLLDNAKLITVESTKIVG
KIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEIST
KVLSIASSYDPLAISRGISNVSMTFIVNKDEGEKLAKELHKVIVSG
>Mature_445_residues
ALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSPNIVTEIYERHIKLLSKLADGK
EFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILSFGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANVI
EDLSKNEIANILENAKANVIVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAKT
ISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLKGISLLDNAKLITVESTKIVGK
IGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSANRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEISTK
VLSIASSYDPLAISRGISNVSMTFIVNKDEGEKLAKELHKVIVSG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=26.133909287257, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790376, Length=447, Percent_Identity=27.0693512304251, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1790455, Length=401, Percent_Identity=28.1795511221945, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320893, Length=184, Percent_Identity=28.2608695652174, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48766; Mature: 48635

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSP
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
NIVTEIYERHIKLLSKLADGKEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
FGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANVIEDLSKNEIANILENAKANV
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
IVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAK
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
TISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECEECCCCCCC
GISLLDNAKLITVESTKIVGKIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSA
CEEEECCCEEEEEECHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEISTKVLSIASSYDPLAISRGISN
HHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCC
VSMTFIVNKDEGEKLAKELHKVIVSG
EEEEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALIVKIGGSIQKDEKDYELIVKKIQDFSKKSDKIIVVTSAIKNVTNELISATSNTDNSP
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
NIVTEIYERHIKLLSKLADGKEFENSFKDISRLSDELFRVAWSIRVLDEVTPRVRDYILS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
FGERMATLLLSAILRSNGIEAEGIITPPFLTDENYGEANVIEDLSKNEIANILENAKANV
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
IVLPGFIGRTREGRYTTLGRGGSDYTATLLGKLVGVREVRLVTEVPGIMTGDPKKFENAK
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHH
TISRLSLEEAIELSQLGAKRLHPRTFDPVFGSDMKVIVESLYEDGFTIINGECQNNDGLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECEECCCCCCC
GISLLDNAKLITVESTKIVGKIGSAARITNEAKEAGVNIISISQPASETTIQLVVDSLSA
CEEEECCCEEEEEECHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
NRLLSKLEEIRGTLVKDIEVNDINIVGIVGCGIKKKEISTKVLSIASSYDPLAISRGISN
HHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCCCC
VSMTFIVNKDEGEKLAKELHKVIVSG
EEEEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]