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The map label for this gene is trpA

Identifier: 15926953

GI number: 15926953

Start: 1378154

End: 1378882

Strand: Direct

Name: trpA

Synonym: SA1205

Alternate gene names: 15926953

Gene position: 1378154-1378882 (Clockwise)

Preceding gene: 15926952

Following gene: 15926954

Centisome position: 48.96

GC content: 34.16

Gene sequence:

>729_bases
ATGACTAAATTATTTATACCTTATATTATGGGCAATAAAGATTTGATTGAAAATGCAACATTGTTGAGTGAAAATGGTGC
AGATATAATTGAAATTGGTGTACCTTTCTCTGATCCGGTTGCTGATGGTCCAGTTATCATGGAAGCAGGTCAACAAGCGA
TTAAACAAGGCATCACGATAGATTATATTTTCAATCAATTAGAAAAACATGGTGATCAAATTAAGTGTAACTATGTATTA
ATGACGTATTATAATATTATTTGTCATTATGGAGAACAAGCGTTTTTTGAAAAATGTCGAGATACTGGTGTCTACGGCTT
AATTATTCCTGATTTACCATATGAATTATCGCAGCGTTTAAAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGT
TAGTTGCGATGACTACTGATGACAAACGTATAAAAGATATCGTATCTCATGCGGAAGGCTTTATTTATACTGTGACGATG
AATGCGACAACAGGACAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCAATTAAAGCGATAGCCAATGT
GCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACATGTTGCAGATATAAAAGAGGTTGCAGATGGCATCGTCATTG
GTAGCGAAATCGTTAAGCGATTTAAATCTAACACGCGTGAGGAAATCATTAGATATTTACAATCTATCCAACAAACATTG
AATAATTAA

Upstream 100 bases:

>100_bases
ACGATGTCGAAAGAAGATATTATTGTAGTAACTATTTCTGGACGTGGCGATAAAGATATGGAAACAATTAGACAATATAT
GGCAGAGCGAGGTCTTGCAA

Downstream 100 bases:

>100_bases
GTTTACTTGATTTAAAAAAATTAGGCGAATACTGTTTGAAAAAGTGAAAAACGGTGAATTATAAAATTGAATAGAAATGA
TACAAAAGTAATATGAGCAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVL
MTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTL
NN

Sequences:

>Translated_242_residues
MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVL
MTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTL
NN
>Mature_241_residues
TKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLM
TYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMN
ATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTLN
N

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=206, Percent_Identity=31.0679611650485, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321412, Length=209, Percent_Identity=35.8851674641148, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_STAA1 (A7X240)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441951.1
- ProteinModelPortal:   A7X240
- SMR:   A7X240
- STRING:   A7X240
- EnsemblBacteria:   EBSTAT00000003255
- GeneID:   5560978
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1361
- eggNOG:   COG0159
- GeneTree:   EBGT00050000024876
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   SAUR418127:SAHV_1361-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27146; Mature: 27014

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 31-31 ACT_SITE 42-42

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITI
CCEEEEEEEECCHHHHHCCHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCH
DYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRL
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCHHHHHHH
KQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMNATTGQNGAFHPELKRKIES
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
IKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTL
HHHHHCCCEEECCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
NN
CC
>Mature Secondary Structure 
TKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITI
CEEEEEEEECCHHHHHCCHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCH
DYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRL
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEEECCCCHHHHHHH
KQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMNATTGQNGAFHPELKRKIES
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
IKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIRYLQSIQQTL
HHHHHCCCEEECCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHCCCHHHHHHHHHHHHHHH
NN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA