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The map label for this gene is miaA

Identifier: 15926886

GI number: 15926886

Start: 1302047

End: 1302982

Strand: Direct

Name: miaA

Synonym: SA1144

Alternate gene names: 15926886

Gene position: 1302047-1302982 (Clockwise)

Preceding gene: 15926885

Following gene: 15926887

Centisome position: 46.26

GC content: 31.84

Gene sequence:

>936_bases
ATGAATAAAAATAAGCCTTTTATTGTAGTAATTGTGGGGCCAACTGCTTCAGGTAAAACAGAGCTTAGCATAGAACTCGC
GAAGCGTATCAATGGTGAAATCATAAGCGGTGATTCTATGCAAGTTTACAAACATATGAATATTGGAACTGCAAAAGTAA
CACCTGAAGAAATGGATGGTATTCCACATCATTTAATTGATATCTTGAATCCTGATGATACATTTTCAGCATATGAATTC
AAGCGATTAGCAGAAGATTTAATTACTGATATAACGAATAGAGGTAAAGTTCCAATCATAGCAGGTGGAACAGGCTTATA
TATTCAATCATTAATATATAATTATGAATTAGAAGATGAAACAGTTACACCTGCACAATTATCCATAGTTAAACAAAAGT
TATCTGCATTAGAACATTTAGATAATCAGCAACTACACGATTATTTAGCTCAATTTGATGCGGTTTCTGCAGAAAATATT
CACCCTAACAACCGCCAAAGAGTGTTGCGCGCTATTGAATATTATTTAAAAACAAAAAAACTTTTGAGTAATCGCAAGAA
AGTGCAACAATTTACTGAAAATTATGATACATTATTATTAGGGATTGAAATGTCGCGTAAAACATTATATTCAAGAATAA
ATAAACGTGTTGATATTATGTTGGATCACGGATTATTTAGAGAAGTGCAACAACTTGTTGAACAAGGCTATGAATCTTGC
CAAAGTATGCAAGCTATTGGATATAAAGAATTAATACCTGTGATTAACGGACAAATGATTTATGAAGATGCTGTCAATGA
TTTAAAGCAACATTCACGCCAATATGCAAAACGACAAATGACATGGTTCAAGAATAAAATGAGTGTTCATTGGTTAGATA
AAGAAAATATGTCACTTCAAATGATGTTAGATGAGATTACAACCCAGATTAAGTAA

Upstream 100 bases:

>100_bases
GTCATGAAGTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAATTTTATAAAAAA
TAAAGAAAGTGGAATTAAAT

Downstream 100 bases:

>100_bases
AAGGAGTCCGACAGATGATTGCAAACGAAAACATCCAAGACAAAGCACTAGAGAATTTTAAAGCAAACCAAACTGAAGTA
ACTGTATTCTTTCTAAACGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEF
KRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESC
QSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

Sequences:

>Translated_311_residues
MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEF
KRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESC
QSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK
>Mature_311_residues
MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEF
KRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESC
QSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=30, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=36.8243243243243, Blast_Score=184, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=33, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6324848, Length=310, Percent_Identity=35.1612903225806, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24649830, Length=340, Percent_Identity=33.8235294117647, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_STAA1 (A7X1U8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441883.1
- ProteinModelPortal:   A7X1U8
- SMR:   A7X1U8
- STRING:   A7X1U8
- EnsemblBacteria:   EBSTAT00000003847
- GeneID:   5560632
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1293
- eggNOG:   COG0324
- GeneTree:   EBGT00050000023843
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   SAUR418127:SAHV_1293-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35869; Mature: 35869

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDG
CCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCEEHHHCCCCCCCCCHHHHCC
IPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDE
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCC
TVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENIHPNNRQRVLRAIEYYLKTKK
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESC
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHHHHHH
QSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQ
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
MMLDEITTQIK
HHHHHHHHHCC
>Mature Secondary Structure
MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDG
CCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCEEHHHCCCCCCCCCHHHHCC
IPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDE
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCC
TVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENIHPNNRQRVLRAIEYYLKTKK
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQGYESC
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHHHHHH
QSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQ
HHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
MMLDEITTQIK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA