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The map label for this gene is purH

Identifier: 15926659

GI number: 15926659

Start: 1050088

End: 1051569

Strand: Direct

Name: purH

Synonym: SA0925

Alternate gene names: 15926659

Gene position: 1050088-1051569 (Clockwise)

Preceding gene: 15926658

Following gene: 15926660

Centisome position: 37.31

GC content: 34.75

Gene sequence:

>1482_bases
GTGATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCGTTAACGCAATTAAATTA
TGAATTATATTCAACTGGTGGTACTAAACGTATATTAGATGAAGCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACAC
ATTTTCCAGAAATAATGGATGGCCGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAA
CCGCAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAATTTATATCCATTCCAACA
AACTGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAAAATATTGATATTGGTGGTCCAACAATGTTACGTGCTG
CAGCTAAAAACTATAAACATGTAACAACAATTGTACATCCGGCAGATTATCATGAAGTATTGACGCGATTAAGAAACGAT
TCGTTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAATATGATGAAGCGATTGTACG
TTTCTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAATCCACAACAATCAGCGTATTTTGTGAGAACTTCGAATG
CTAAGCACACGATTGCAGGTGCTAAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACA
CTAGCTTTAGTTAAAAAGTTTGATACACCTGCTGCAGTTGCGGTTAAACACATGAATCCATGTGGTGTTGGTATCGGTGA
CACGATTGAACAAGCATTTCAACATGCGTATGAAGCGGATAGTCAATCAATATTTGGTGGAATTGTTGCATTAAACCGAG
CTGTAACACCTGAGTTAGCAGAGCAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCA
TTAGATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGTAACGAAGAAGAGTTTGT
TTCAGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTATGTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAG
TAGCACCTACTGATGAACAATGGGAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATT
TTAAGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCTAAAATTGCGTTAGAGAG
AGCTATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGATTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCAC
AACATGGTATAAAGGCAATTATCCAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGT
ATTGCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA

Upstream 100 bases:

>100_bases
CGGACGATTCAAAAGAACAATTAGAAGAGAAAGTAAAAAAATTGGAATATGAGTTATATCCAAGTGTTATTGCTAAAATT
GTAAAATAAGGAGTATGTAT

Downstream 100 bases:

>100_bases
TTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAA
TCAATCGAATCTAGTTAAAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNK
PQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRND
SLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADAT
LALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEA
LDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAII
LSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHG
IAMVVTGTRHFKH

Sequences:

>Translated_493_residues
MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNK
PQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRND
SLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADAT
LALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEA
LDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAII
LSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHG
IAMVVTGTRHFKH
>Mature_493_residues
MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNK
PQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRND
SLDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADAT
LALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEA
LDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAII
LSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHG
IAMVVTGTRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=461, Percent_Identity=38.8286334056399, Blast_Score=272, Evalue=7e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=47.2380952380952, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71985564, Length=466, Percent_Identity=34.1201716738197, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=460, Percent_Identity=37.3913043478261, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=36.4605543710021, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=463, Percent_Identity=37.1490280777538, Blast_Score=258, Evalue=9e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54459; Mature: 54459

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMD
CCCHHHEECCCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHC
GRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAI
CCHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
ENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDSLDESYRQSLMIKVFEHTAE
HHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADAT
HHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHH
LALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELA
HHHHHHCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHH
EQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDN
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCC
YVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGA
EECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHH
AKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHG
HHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC
IAMVVTGTRHFKH
EEEEEECCCCCCC
>Mature Secondary Structure
MMKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMD
CCCHHHEECCCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHC
GRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAI
CCHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHH
ENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDSLDESYRQSLMIKVFEHTAE
HHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADAT
HHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHH
LALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELA
HHHHHHCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHH
EQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDN
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCC
YVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGA
EECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHH
AKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHG
HHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC
IAMVVTGTRHFKH
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA