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The map label for this gene is gltX

Identifier: 15926205

GI number: 15926205

Start: 568646

End: 570100

Strand: Direct

Name: gltX

Synonym: SA0486

Alternate gene names: 15926205

Gene position: 568646-570100 (Clockwise)

Preceding gene: 15926204

Following gene: 15926206

Centisome position: 20.2

GC content: 34.23

Gene sequence:

>1455_bases
ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGTTATCTTCATATTGGTAATGCAAGAACAGCATTATT
CAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTGATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAG
ATGGAGAAACATCACAATTTGATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTAGCAGAAGATAAAGCATATAA
ATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAAGCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTC
AACATGCGCATTTGACTGAAGAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAATGGTATTGGTGACTGGGTTAT
CGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTAGCTATAGATGATCATTACATGCAAATTTCAGATGTAATTC
GTGGTGATGATCATATTTCAAACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATTTTACAATTTATTGAGCAATA
TCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTTATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAA
TCTTTTCTAAAGAAGAATTTATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTAGCATTACCTCATTTAATTAA
AGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTATCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAA
TGAGTTATGCCGGTGAAATTGTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
GTGATTAATGGAGAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCACTTGAACCATTTGAAGCGGC
TGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACAGGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTG
CTGTAACAGGCCAAATGCATGGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
AAACAATATAAGTAA

Upstream 100 bases:

>100_bases
TAAGACGAGTTAGACATTTGTTATGACGGTCGAAGATAGCAAATGAATAATTTATATGAACTAACTTGAAAATCTGATAT
TTAATTTAGGAGTGAAAAAA

Downstream 100 bases:

>100_bases
TGAAATAGACAAGATCAAGGTAGTATATACTTGAATTTTATAACGTAACCACATATGATAAAGTTGTATTAACAAATTAC
AACAATATGAATAAAGTATT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK

Sequences:

>Translated_484_residues
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGY
GPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQK
LAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQ
VINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
KQYK
>Mature_483_residues
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYG
PYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQ
NQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGH
MSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKL
AWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQV
INGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLK
QYK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=31.0204081632653, Blast_Score=251, Evalue=1e-66,
Organism=Escherichia coli, GI1788743, Length=480, Percent_Identity=37.9166666666667, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=29.8804780876494, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=32.5819672131148, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=32.9341317365269, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321192, Length=213, Percent_Identity=25.8215962441315, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=146, Percent_Identity=31.5068493150685, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=32.9979879275654, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_STAA1 (A7WYU4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441115.1
- ProteinModelPortal:   A7WYU4
- SMR:   A7WYU4
- STRING:   A7WYU4
- EnsemblBacteria:   EBSTAT00000004048
- GeneID:   5559018
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0525
- eggNOG:   COG0008
- GeneTree:   EBGT00050000024594
- HOGENOM:   HBG628189
- OMA:   MEGVPIM
- ProtClustDB:   PRK01406
- BioCyc:   SAUR418127:SAHV_0525-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56290; Mature: 56158

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
LEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC
>Mature Secondary Structure 
SDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQF
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCC
DNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAERE
CCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
AQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSN
HHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCCCCCC
GIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFG
CCCCEEEEECCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCC
HMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEF
CEEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDL
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
SWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHH
LEPFEAAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
KQYK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA