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The map label for this gene is aspS

Identifier: 15675898

GI number: 15675898

Start: 1795501

End: 1797249

Strand: Reverse

Name: aspS

Synonym: SPy_2156

Alternate gene names: 15675898

Gene position: 1797249-1795501 (Counterclockwise)

Preceding gene: 15675899

Following gene: 15675897

Centisome position: 97.02

GC content: 42.14

Gene sequence:

>1749_bases
ATGAAACGTAGCATGTATGCAGGGCGTGTTCGCGAAGAACACATTGGAACGACAATCACATTGAAAGGATGGGTCAGTCG
TCGTCGTGACTTGGGTGGTCTCATCTTTATTGATTTGCGTGACCGTGAGGGTGTGATGCAATTAGTCATTAACCCTGAGG
AAGTTTCCAGTGATGTGATGGCAACTGCTGAACGCTTACGTAGTGAATATGTGATTGAAGTGGAAGGTTTTGTTGAAGCT
CGACAACAAGCCAATGATAAGTTGGCGACAGGAATGGTTGAGTTAAAAGTATCTGCTTTGACCATTTTAAACACAGCTAA
AACGACGCCTTTTGAAATTAAAGATGACGTTGAAGTAAGTGATGACACGCGTTTACGTTACCGTTACTTAGATTTACGAC
GTCCAGAAATGTTAGAAAACTTCAAGCTACGTGCAAAAGTGACTCATTCTATCCGTAATTACCTTGATGACCTTGAGTTT
ATCGATGTCGAGACACCTATGTTGACAAAATCAACTCCAGAAGGTGCGCGTGACTATCTGGTACCAAGTCGTGTTAGCCA
AGGGCATTTCTATGCTTTGCCACAAAGTCCGCAAATAACCAAACAGTTATTGATGAATGCTGGCTTTGATCGCTATTATC
AAATTGTGAAATGTTTCCGCGATGAAGATCTACGTGGTGACCGTCAACCTGAGTTTACACAAGTTGACTTGGAAACGTCA
TTCCTTTCTGAACAAGAAATTCAAGACATCGTTGAAGGCATGATTGCTAAAGTGATGAAAGAGACAAAAGAAATAGATGT
AACCCTACCATTCCCACGTATGAGCTACGATGTAGCAATGAATAGCTACGGCTCTGATAAACCAGACACACGTTTTGAAA
TGCTTTTACAAGACTTGACAGTAACTGTCAAAGGAAATGACTTCAAGGTTTTCTCAGAAGCACCAGCTGTCAAGGCGATT
GTGGTGAAGGGAAATGCAGATCGATACTCTCGTAAAGATATTGACAAATTAACTGAGTTTGCCAAACAATTTGGTGCTAA
AGGCCTTGCCTGGGTTAAGGTGACAGATGGTCAATTGGCAGGCCCAGTAGCCAAGTTCTTGACAGCTATTGAGACAGAGT
TGTCAAGCCAATTAAAACTTGCTGAGAATGATTTGGTTTTATTTGTAGCGGATACTCTTGAGGTTGCTAATAATACATTA
GGTGCCCTTCGTAATCGTATTGCCAAGGACCTTGACATGATTGATCAGTCGCAGTTTAATTTCCTTTGGGTGGTTGATTG
GCCAATGTTTGAGTGGTCAGAAGAAGAAGGACGTTATATGTCAGCTCATCACCCCTTCACTCTTCCAACGCCAGAATCTG
CTCATGAGTTAGAGGGTGATTTGGCTAAGGTACGGGCTATTGCTTACGACATCGTTTTAAATGGTTACGAGCTTGGTGGT
GGTAGCCTGCGTATCAATCAAAAAGAGATGCAAGAGCGCATGTTCAAGGCTCTTGGCTTTACCGCTGATGAAGCTAACGA
CCAATTTGGTTTCTTACTAGAAGCGATGGACTACGGCTTTCCTCCTCATGGTGGCTTGGCAATTGGTCTTGACCGTTTTG
TCATGTTGCTTGCTGGAAAAGACAATATTCGAGAAGTTATTGCCTTCCCTAAAAATAACAAAGCCTCTGATCCAATGACG
CAAGCACCTAGTCTTGTTTCTGAAAACCAATTAGAAGAACTCAGTCTTCAAATAGAAAGTCATGATTAA

Upstream 100 bases:

>100_bases
TTGCTATAATAGTAGGGAATGAATTAATTGATTTATATAGTGAAAGGAATTGGAAACGAGCAGGTTTCACAGGATTCTGT
TTGTTTTAGGGAATTTATTT

Downstream 100 bases:

>100_bases
GAAAACAACCTATAAGAAAAAAGTTAAATATGTCATTAGTAGAGGGGCCAAGAAAGTTGGCCTACTCCACGCTCTAAGAA
GTATTTCAAGAGAAAAATAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD

Sequences:

>Translated_582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD
>Mature_582_residues
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEA
RQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLTVTVKGNDFKVFSEAPAVKAI
VVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTL
GALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMT
QAPSLVSENQLEELSLQIESHD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=592, Percent_Identity=40.3716216216216, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI5031815, Length=285, Percent_Identity=27.0175438596491, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194272210, Length=285, Percent_Identity=27.0175438596491, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI45439306, Length=275, Percent_Identity=26.9090909090909, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=49.3220338983051, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1789256, Length=294, Percent_Identity=25.5102040816327, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790571, Length=295, Percent_Identity=24.406779661017, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=589, Percent_Identity=35.9932088285229, Blast_Score=322, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI71994340, Length=310, Percent_Identity=22.9032258064516, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=308, Percent_Identity=23.0519480519481, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=310, Percent_Identity=22.9032258064516, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=264, Percent_Identity=25.3787878787879, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=24.8344370860927, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=625, Percent_Identity=32.32, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=241, Percent_Identity=28.6307053941909, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=256, Percent_Identity=26.171875, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321807, Length=267, Percent_Identity=24.3445692883895, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=36, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=28.4518828451883, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640851, Length=279, Percent_Identity=27.2401433691756, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640849, Length=278, Percent_Identity=26.2589928057554, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_STRP1 (Q99XL0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270072.1
- RefSeq:   YP_283176.1
- ProteinModelPortal:   Q99XL0
- SMR:   Q99XL0
- EnsemblBacteria:   EBSTRT00000001462
- EnsemblBacteria:   EBSTRT00000028068
- GeneID:   3571061
- GeneID:   901791
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2156
- KEGG:   spz:M5005_Spy_1813
- GeneTree:   EBGT00050000027607
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   SPYO160490:SPY2156-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1813-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66029; Mature: 66029

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSDVM
CCCCCCCCCCHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVS
HHHHHHCCCEEEEEHHHHHHHHHCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEFIDVETPMLTKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHCC
VPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLT
HCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHCE
VTVKGNDFKVFSEAPAVKAIVVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLA
EEEECCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
GPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTLGALRNRIAKDLDMIDQSQFN
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGK
CEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
DNIREVIAFPKNNKASDPMTQAPSLVSENQLEELSLQIESHD
CCHHHHHCCCCCCCCCCCCHHCCCHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREGVMQLVINPEEVSSDVM
CCCCCCCCCCHHHHCCCEEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTILNTAKTTPFEIKDDVEVS
HHHHHHCCCEEEEEHHHHHHHHHCCHHHHHCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEFIDVETPMLTKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHCC
VPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSYDVAMNSYGSDKPDTRFEMLLQDLT
HCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHCE
VTVKGNDFKVFSEAPAVKAIVVKGNADRYSRKDIDKLTEFAKQFGAKGLAWVKVTDGQLA
EEEECCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
GPVAKFLTAIETELSSQLKLAENDLVLFVADTLEVANNTLGALRNRIAKDLDMIDQSQFN
HHHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPTPESAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKEMQERMFKALGFTADEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGK
CEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
DNIREVIAFPKNNKASDPMTQAPSLVSENQLEELSLQIESHD
CCHHHHHCCCCCCCCCCCCHHCCCHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296