Click here to switch to the map view.

The map label for this gene is fhs.2

Identifier: 15675843

GI number: 15675843

Start: 1736474

End: 1738147

Strand: Direct

Name: fhs.2

Synonym: SPy_2085

Alternate gene names: 15675843

Gene position: 1736474-1738147 (Clockwise)

Preceding gene: 15675842

Following gene: 15675844

Centisome position: 93.74

GC content: 47.19

Gene sequence:

>1674_bases
ATGGTTTTATCAGATATTGAAATAGCCAACTCTGTCACTATGGAACCTATCAGTAAGGTTGCCGATCAATTAGGGATTGA
CAAGGAGGCGCTCTGTCTCTATGGTAAGTACAAGGCAAAAATTGATGCCCGTCAACTGGTCGCCTTAAAAAACAAACCTG
ATGGCAAACTCATTCTTGTCACTGCTATCTCACCAACACCAGCTGGTGAAGGCAAAACCACCACTTCTGTTGGATTGGTA
GATGCTCTTTCTGCCATTGGAAAAAAAGCAGTTATTGCCCTTCGTGAACCTTCTCTTGGTCCTGTTTTTGGGGTTAAGGG
AGGAGCTGCTGGCGGTGGCCACGCGCAAGTTGTCCCAATGGAAGACATCAACCTTCACTTTACAGGGGACTTTCATGCCA
TCGGTGTCGCCAACAACTTATTAGCTGCCTTGATTGATAACCACATTCACCATGGCAATAGCCTAGGCATTGATTCCAGA
CGTATCACTTGGAAACGTGTGGTGGATATGAATGACCGCCAGCTTCGTCATATTGTTGACGGCTTGCAAGGCAAGGTCAA
CGGGATTCCTCGTGAAGATGGTTATGACATTACCGTCGCCTCTGAAATCATGGCAATCCTGTGCCTATCCGAAAATATTT
CTGACCTCAAAGCCCGCCTTGAAAAAATCATCATCGGTTACAACTACCAAGGTGAACCTGTAACAGCTAAAGACTTGAAA
GCTGGTGGTGCCCTAGCCGCTCTGCTCAAGGATGCGATTCATCCTAATCTCGTGCAAACCCTGGAACACACACCAGCCCT
CATTCACGGTGGCCCATTTGCTAATATCGCCCATGGCTGTAATAGTGTCCTCGCCACCAAACTAGCCTTGAAATATGGTG
ATTATGCCGTTACCGAAGCTGGATTTGGCGCTGACCTTGGAGCTGAAAAATTCATTGACATCAAATGCCGCATGTCAGGC
CTTCGCCCAGCAGCCGTTGTCCTAGTAGCCACTATACGTGCCCTTAAAATGCATGGGGGTGTGCCAAAAGCAGACCTGGC
TACTGAAAATGTTCAGGCCGTTGTGGATGGTTTACCTAACCTTGACAAACATTTGGCTAATATCCAAGACGTCTATGGCC
TTCCAGTTGTCGTGGCGATCAATAAATTCCCGCTTGATACCGATGCCGAATTACAAGCTGTCTATGATGCCTGCGACAAA
CGTGGCGTTGACGTTGTGATTTCTGATGTTTGGGCAAATGGCGGAGCTGGTGGCCGTGAACTGGCTGAAAAAGTCGTGAC
ACTAGCCGAGCAAGACAATCAATTCCGCTTTGTTTATGAGGAAGACGATAGCATTGAAACCAAACTGACTAAAATCGTTA
CCAAAGTTTACGGTGGTAAAGGCATCAACTTGAGCTCAGCAGCCAAACGTGAACTGGCTGACTTAGAACGCCTCGGCTTT
GGCAACTACCCAATTTGCATGGCCAAAACCCAATATTCCTTCTCAGACGATGCCAAAAAACTTGGCGCACCAACTGACTT
TACAGTGACTATTAGCAACCTTAAAGTGTCCGCAGGAGCAGGATTTATCGTTGCCCTAACAGGTGCTATCATGACCATGC
CTGGCCTTCCAAAAGTACCTGCCAGCGAAACAATTGATATTGACGAGGAGGGCAACATCACAGGACTATTCTAA

Upstream 100 bases:

>100_bases
ATCGCCAAAAAGGACAAGCCTTACTAGACAAGGGCTGTCATTTGGCAGATGACATCTACACGAAAATTCTCGATATTGTT
TAACAAGTAAAGGAGCAACT

Downstream 100 bases:

>100_bases
TTGGAAATAAGGAGAGAACAAATGACAAAGGTAACCATCAAAGCGCCCTCTGATTACCTCCAAACTGACTGGTCTGGCGG
TGAAACCAACCAATTGTTCC

Product: formate--tetrahydrofolate ligase

Products: NA

Alternate protein names: Formyltetrahydrofolate synthetase 2; FHS 2; FTHFS 2

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDARQLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLV
DALSAIGKKAVIALREPSLGPVFGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
RITWKRVVDMNDRQLRHIVDGLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKARLEKIIIGYNYQGEPVTAKDLK
AGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANIAHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSG
LRPAAVVLVATIRALKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQAVYDACDK
RGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLTKIVTKVYGGKGINLSSAAKRELADLERLGF
GNYPICMAKTQYSFSDDAKKLGAPTDFTVTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF

Sequences:

>Translated_557_residues
MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDARQLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLV
DALSAIGKKAVIALREPSLGPVFGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
RITWKRVVDMNDRQLRHIVDGLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKARLEKIIIGYNYQGEPVTAKDLK
AGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANIAHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSG
LRPAAVVLVATIRALKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQAVYDACDK
RGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLTKIVTKVYGGKGINLSSAAKRELADLERLGF
GNYPICMAKTQYSFSDDAKKLGAPTDFTVTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF
>Mature_557_residues
MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDARQLVALKNKPDGKLILVTAISPTPAGEGKTTTSVGLV
DALSAIGKKAVIALREPSLGPVFGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
RITWKRVVDMNDRQLRHIVDGLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKARLEKIIIGYNYQGEPVTAKDLK
AGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANIAHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSG
LRPAAVVLVATIRALKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQAVYDACDK
RGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLTKIVTKVYGGKGINLSSAAKRELADLERLGF
GNYPICMAKTQYSFSDDAKKLGAPTDFTVTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF

Specific function: Unknown

COG id: COG2759

COG function: function code F; Formyltetrahydrofolate synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate--tetrahydrofolate ligase family

Homologues:

Organism=Homo sapiens, GI36796743, Length=621, Percent_Identity=46.0547504025765, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI222136639, Length=622, Percent_Identity=44.2122186495177, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI310124614, Length=84, Percent_Identity=45.2380952380952, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568737, Length=625, Percent_Identity=45.44, Blast_Score=463, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17568739, Length=625, Percent_Identity=45.44, Blast_Score=463, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321643, Length=626, Percent_Identity=44.7284345047923, Blast_Score=472, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6319558, Length=633, Percent_Identity=42.8120063191153, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI62472483, Length=621, Percent_Identity=45.8937198067633, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI45551871, Length=621, Percent_Identity=45.8937198067633, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24645718, Length=621, Percent_Identity=45.8937198067633, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17137370, Length=621, Percent_Identity=45.8937198067633, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FTHS2_STRP1 (Q99XR2)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_270017.1
- RefSeq:   YP_283137.1
- ProteinModelPortal:   Q99XR2
- SMR:   Q99XR2
- EnsemblBacteria:   EBSTRT00000000998
- EnsemblBacteria:   EBSTRT00000028183
- GeneID:   3571100
- GeneID:   901726
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_2085
- KEGG:   spz:M5005_Spy_1774
- GeneTree:   EBGT00050000027107
- HOGENOM:   HBG677721
- OMA:   KMPGLPK
- ProtClustDB:   PRK13505
- BioCyc:   SPYO160490:SPY2085-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1774-MONOMER
- HAMAP:   MF_01543
- InterPro:   IPR000559
- InterPro:   IPR020628

Pfam domain/function: PF01268 FTHFS

EC number: =6.3.4.3

Molecular weight: Translated: 59055; Mature: 59055

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00721 FTHFS_1; PS00722 FTHFS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDARQLVALKNKPDGKLILV
CEECCCHHCCCCCHHHHHHHHHHHCCCHHHEEEEECHHCCCCHHHHEEECCCCCCCEEEE
TAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPVFGVKGGAAGGGHAQVVPM
EEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCEEEEEE
EDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSRRITWKRVVDMNDRQLRHIVD
CCCEEEEECCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
GLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKARLEKIIIGYNYQGEPVTAKDLK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCHHHHC
AGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANIAHGCNSVLATKLALKYGDYAVTEA
CCHHHHHHHHHHCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECC
GFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRALKMHGGVPKADLATENVQAVVDGLPN
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
LDKHLANIQDVYGLPVVVAINKFPLDTDAELQAVYDACDKRGVDVVISDVWANGGAGGRE
HHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHH
LAEKVVTLAEQDNQFRFVYEEDDSIETKLTKIVTKVYGGKGINLSSAAKRELADLERLGF
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GNYPICMAKTQYSFSDDAKKLGAPTDFTVTISNLKVSAGAGFIVALTGAIMTMPGLPKVP
CCCCEEEEECCCCCCCHHHHCCCCCCEEEEEEEEEEECCCCEEEEEHHHHHHCCCCCCCC
ASETIDIDEEGNITGLF
CCCEECCCCCCCEEECC
>Mature Secondary Structure
MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDARQLVALKNKPDGKLILV
CEECCCHHCCCCCHHHHHHHHHHHCCCHHHEEEEECHHCCCCHHHHEEECCCCCCCEEEE
TAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPVFGVKGGAAGGGHAQVVPM
EEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCEEEEEE
EDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSRRITWKRVVDMNDRQLRHIVD
CCCEEEEECCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
GLQGKVNGIPREDGYDITVASEIMAILCLSENISDLKARLEKIIIGYNYQGEPVTAKDLK
HHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCHHHHC
AGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANIAHGCNSVLATKLALKYGDYAVTEA
CCHHHHHHHHHHCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECC
GFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRALKMHGGVPKADLATENVQAVVDGLPN
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
LDKHLANIQDVYGLPVVVAINKFPLDTDAELQAVYDACDKRGVDVVISDVWANGGAGGRE
HHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHH
LAEKVVTLAEQDNQFRFVYEEDDSIETKLTKIVTKVYGGKGINLSSAAKRELADLERLGF
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GNYPICMAKTQYSFSDDAKKLGAPTDFTVTISNLKVSAGAGFIVALTGAIMTMPGLPKVP
CCCCEEEEECCCCCCCHHHHCCCCCCEEEEEEEEEEECCCCEEEEEHHHHHHCCCCCCCC
ASETIDIDEEGNITGLF
CCCEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296