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The map label for this gene is truA

Identifier: 15675714

GI number: 15675714

Start: 1576300

End: 1577049

Strand: Reverse

Name: truA

Synonym: SPy_1901

Alternate gene names: 15675714

Gene position: 1577049-1576300 (Counterclockwise)

Preceding gene: 15675715

Following gene: 15675713

Centisome position: 85.13

GC content: 36.13

Gene sequence:

>750_bases
ATGGTAAGATACAAAGCAACGATTTCCTATGATGGGACTCTTTTTTCGGGTTTTCAAAGACAAAGACATTTACGTACGGT
TCAAGAAGAGATTGAAAAAACACTCTATAAATTAAATAACGGTACAAAAATTATAATTCATGGTGCAGGACGTACGGATG
CAGGAGTACATGCTTATGGACAGGTGATCCATTTTGATTTGCCTCAAGAACAAGAAGTTGAAAAATTGCGATTTGCTCTT
GATACACAGACTCCTGAAGATATTGATGTTGTTAATATAGAAAAAGTGGCTGATGATTTTCATTGTCGTTATCAGAAGCA
TTTAAAAACTTATGAATTTTTAGTTGATAATGGTCGACCTAAAAATCCGATGATGCGACACTATACCACTCATTACCCTT
ATACTCTTAATATTAAACTTATGCAAGAAGCTATTAACGGTCTGGTAGGTACACACGATTTTACTGGTTTTACAGCAGCA
GGAACTTCGGTACAAAATAAGGTGCGAACGATTACCAAAGCAACGGTATCTCGGGATGAAAAAACTGATTTTTTGGTGTT
TACTTTTTCAGGAAATGGTTTTCTCTATAAGCAGGTGAGGAATATGGTGGGAACTTTGCTGAAAATTGGTAATGGTCAAA
TGCCAGTTGAACAAGTTAAAGTGATTTTGAGCTCCAAAAATCGTCAGCTAGCTGGACCAACGATATCTGGAAATGGGCTT
TACTTGAAGGAGATTTGTTATGAAAACTGA

Upstream 100 bases:

>100_bases
CTAGTAGCTTAGAGTAAGCTTCAATACGTCTTTCATTTGTTTGGGTACAGTTTGATTAAAAATAGTTTCTCTTACGTTTA
AGAAATGTTAAAATAGTAAC

Downstream 100 bases:

>100_bases
TTATATCGTGACTATATCTGGGAACGATATTTTAAGTGGAGGTGGTCTCTATGCTGATTTAGCGACTTACATAAGGTATG
ACCTACAGGCCTTTGTAGCG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKIIIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFAL
DTQTPEDIDVVNIEKVADDFHCRYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQVKVILSSKNRQLAGPTISGNGL
YLKEICYEN

Sequences:

>Translated_249_residues
MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKIIIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFAL
DTQTPEDIDVVNIEKVADDFHCRYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQVKVILSSKNRQLAGPTISGNGL
YLKEICYEN
>Mature_249_residues
MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKIIIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFAL
DTQTPEDIDVVNIEKVADDFHCRYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQVKVILSSKNRQLAGPTISGNGL
YLKEICYEN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=278, Percent_Identity=32.0143884892086, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI199559785, Length=261, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=33.4710743801653, Blast_Score=155, Evalue=2e-39,
Organism=Drosophila melanogaster, GI116007752, Length=308, Percent_Identity=27.9220779220779, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362146, Length=257, Percent_Identity=25.2918287937743, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648455, Length=257, Percent_Identity=25.2918287937743, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_STRP1 (P65850)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269888.1
- RefSeq:   YP_282980.1
- ProteinModelPortal:   P65850
- SMR:   P65850
- EnsemblBacteria:   EBSTRT00000000840
- EnsemblBacteria:   EBSTRT00000029082
- GeneID:   3571276
- GeneID:   901587
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1901
- KEGG:   spz:M5005_Spy_1617
- GeneTree:   EBGT00050000026796
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   SPYO160490:SPY1901-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1617-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28357; Mature: 28357

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKIIIHGAGRTDAGVHAYG
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCC
QVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHCRYQKHLKTYEFLVDNGRP
EEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEHHCCCCCC
KNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAAGTSVQNKVRTITKATVSRDE
CCCHHHHEECCCCEEEEHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
KTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQVKVILSSKNRQLAGPTISGNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCEECCCCE
YLKEICYEN
EEHHHHCCC
>Mature Secondary Structure
MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKIIIHGAGRTDAGVHAYG
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCC
QVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHCRYQKHLKTYEFLVDNGRP
EEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEHHCCCCCC
KNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAAGTSVQNKVRTITKATVSRDE
CCCHHHHEECCCCEEEEHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
KTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQVKVILSSKNRQLAGPTISGNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEECCCEECCCCE
YLKEICYEN
EEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296