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The map label for this gene is serS

Identifier: 15675591

GI number: 15675591

Start: 1443570

End: 1444847

Strand: Direct

Name: serS

Synonym: SPy_1742

Alternate gene names: 15675591

Gene position: 1443570-1444847 (Clockwise)

Preceding gene: 15675590

Following gene: 15675620

Centisome position: 77.93

GC content: 42.33

Gene sequence:

>1278_bases
ATGCTAGATCTTAAACGTATCCGTACCGATTTTGACACTGTCGCTGCCAAATTAAAAAACCGCGGTGTTTCTGAAGACAC
CCTGACCCATCTTAAAGAACTTGATGAAAAACGTCGTGCTCTATTAGTGCAATCAGAAGAATTAAAAGCAGAGCGTAATA
TTGCTTCTGCAGCCATCGCCCAAGCAAAACGTCAAAAAGAAGATGCCACTCAACAAATTGCCGATATGCAAAAAGTCTCT
GCAGATATCAAAACTATTGACAATCAACTAGTAGCTATCGACCAGCAAGTTACTGACATCATCACTGTTTTACCTAATAC
TCCTCACGATTCTGTCCCTGTTGGAGCTGACGAAGAAGACAATGTCGAAATTCGCCGTTGGGGAACGCCTCGTGACTTTG
ACTTCGAAGTTAAGGCTCATTGGGATCTAGGCGAAGATCTTGATATTTTAGACTGGGAACGTGGGGCAAAGGTAACTGGT
GCTCGTTTCTTATTCTATAAAAATCTTGGTGCTCGTTTGGAGCGTGCTCTTTATAACTTCATGCTGGATGAACATATCAA
AGAAGGTTATCAAGAAATCATCACACCTTACATGGTTAACCACGATTCCATGTTTGGTACAGGACAATATCCAAAATTCA
AAGAGGACACCTTTGAATTAGCTGATACCAACTTTGTCCTGATTCCAACTGCTGAAGTGCCTTTGACCAATTATTACCGT
GGGGAAATCCTAGACGGCAAAGAATTGCCAATTTACTTTACTGCAATGAGCCCATCTTTTCGTTCTGAAGCGGGTTCTGC
GGGTCGTGACACACGTGGCCTTATCCGCCTACACCAATTCCACAAAGTTGAAATGGTTAAATTTGCTAAACCAGAAGAAT
CTTACCAAGAACTAGAAAAAATGACTGCTAATGCTGAAAATATTCTTCAAAAACTTGGTTTGCCATATCGCGTCATTTCC
CTTTGTACTGGAGACATGGGCTTCTCAGCTGCTAAAACCTATGACCTTGAAGTCTGGATTCCAGCTCAAAATACCTATCG
TGAAATTTCATCATGTTCAAATACAGAAGATTTCCAAGCACGTCGTGCTCAAATCCGTTACCGTGATGAGGCTGACGGTA
AGGTCAAACTTCTCCACACCCTTAATGGGTCAGGACTTGCTGTCGGTCGTACCGTAGCAGCCATTTTAGAAAACTACCAA
AATGAAGATGGTTCTGTTACCATCCCTGAAGTACTCCGCCCTTACATGGGTGGCGAAACCGTCATTTCACCTAAATAA

Upstream 100 bases:

>100_bases
GCTGTGCTTTTTCTATCGTCAGAGAATCACAAGACCCAACTGTCTTACTTAGCCTTGTTTCACTGACAGCTACTAATAAC
TATCTTAAAGGAGCTTCTGT

Downstream 100 bases:

>100_bases
TAAATTCACCCCAGAGATCATTAAAGTGATTTCTGGGGTTTACTATTTTAAAGAGCCACCACATCACTTGAAACTCACTA
CATTGCCCCTTTAACAACAC

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQSEELKAERNIASAAIAQAKRQKEDATQQIADMQKVS
ADIKTIDNQLVAIDQQVTDIITVLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTFELADTNFVLIPTAEVPLTNYYR
GEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVIS
LCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTVAAILENYQ
NEDGSVTIPEVLRPYMGGETVISPK

Sequences:

>Translated_425_residues
MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQSEELKAERNIASAAIAQAKRQKEDATQQIADMQKVS
ADIKTIDNQLVAIDQQVTDIITVLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTFELADTNFVLIPTAEVPLTNYYR
GEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVIS
LCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTVAAILENYQ
NEDGSVTIPEVLRPYMGGETVISPK
>Mature_425_residues
MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQSEELKAERNIASAAIAQAKRQKEDATQQIADMQKVS
ADIKTIDNQLVAIDQQVTDIITVLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTFELADTNFVLIPTAEVPLTNYYR
GEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVIS
LCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTVAAILENYQ
NEDGSVTIPEVLRPYMGGETVISPK

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=469, Percent_Identity=31.7697228144989, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI8923421, Length=414, Percent_Identity=32.1256038647343, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI225579074, Length=329, Percent_Identity=34.0425531914894, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1787120, Length=426, Percent_Identity=56.8075117370892, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786392, Length=171, Percent_Identity=26.9005847953216, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539260, Length=429, Percent_Identity=32.1678321678322, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17542084, Length=325, Percent_Identity=29.5384615384615, Blast_Score=154, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320226, Length=396, Percent_Identity=35.3535353535354, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321799, Length=431, Percent_Identity=30.6264501160093, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24581334, Length=330, Percent_Identity=36.0606060606061, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI17737985, Length=347, Percent_Identity=31.4121037463977, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_STRP1 (Q99YE2)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269765.1
- RefSeq:   YP_282846.1
- ProteinModelPortal:   Q99YE2
- SMR:   Q99YE2
- EnsemblBacteria:   EBSTRT00000001047
- EnsemblBacteria:   EBSTRT00000027922
- GeneID:   3571406
- GeneID:   901977
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1742
- KEGG:   spz:M5005_Spy_1483
- GeneTree:   EBGT00050000026593
- HOGENOM:   HBG629391
- OMA:   PKFADDM
- ProtClustDB:   PRK05431
- BioCyc:   SPYO160490:SPY1742-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1483-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 48182; Mature: 48182

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 284-284 BINDING 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQSEELKAERNIASAAIA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHH
QAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIITVLPNTPHDSVPVGADEED
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTGARFLFYKNLGARLERALYNF
CEEEEECCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHHHHHH
MLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTFELADTNFVLIPTAEVPLTNYYR
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCC
GEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEK
CCEECCCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MTANAENILQKLGLPYRVISLCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQA
HHCCHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCHHHHH
RRAQIRYRDEADGKVKLLHTLNGSGLAVGRTVAAILENYQNEDGSVTIPEVLRPYMGGET
HHHHEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCE
VISPK
EECCC
>Mature Secondary Structure
MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQSEELKAERNIASAAIA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHH
QAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIITVLPNTPHDSVPVGADEED
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCCCCCCCCCCCCCCC
NVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTGARFLFYKNLGARLERALYNF
CEEEEECCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHHHHHH
MLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTFELADTNFVLIPTAEVPLTNYYR
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCC
GEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIRLHQFHKVEMVKFAKPEESYQELEK
CCEECCCCCCEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MTANAENILQKLGLPYRVISLCTGDMGFSAAKTYDLEVWIPAQNTYREISSCSNTEDFQA
HHCCHHHHHHHCCCCCEEHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCHHHHH
RRAQIRYRDEADGKVKLLHTLNGSGLAVGRTVAAILENYQNEDGSVTIPEVLRPYMGGET
HHHHEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCE
VISPK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296