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The map label for this gene is ntcA [H]

Identifier: 15675445

GI number: 15675445

Start: 1274950

End: 1275630

Strand: Reverse

Name: ntcA [H]

Synonym: SPy_1548

Alternate gene names: 15675445

Gene position: 1275630-1274950 (Counterclockwise)

Preceding gene: 15675447

Following gene: 15675444

Centisome position: 68.86

GC content: 35.98

Gene sequence:

>681_bases
GTGATTCGAAGAGAAGATTATCAATACCTTAGAAAACTAAACGATTTTCGCTACTTTTCTATTGAACAATTTGACAAAAT
TGTAGGGCAGATGGAATTCCGAAAAGCTAAAAAGGACCATATCCTTTTTTTTGAGGGAGATAAAAGAGATAAGCTTTTTC
TAGTGACCTCTGGTTATTTCAAGGTGGAGCAGTCCGATCAATCAGGAACCTTCATGTATACGGATTTCATCAGACACGGC
ACCATTTTTCCTTACGGTGGCCTCTTTACAGATGATTATTACCATTTTTCAGTGGTAGCCATGACAGACGTGACTTATTT
TTACTTTCCAGTCGATTTATTTGAAGATTATTCGCTTGAAAATCGTCTACAAATGAAACACTTATATAGTAAGATGTCTA
AGCTGCTCGAGTTACATGAGTTGAGGGTCCGAAACTTGATTACATCAAGTGCTAGTTCTCGTGTGATTCAATCTCTGGCG
ATTTTGCTGGTTGAAATGGGTAAAGACAGTGATACCTTACCTTTTCAATTAACAACAACAGATATTGCTCAAATAAGTGG
GACGACAAGAGAAACAGTTAGCCATGTGCTTAGAGATTTGAAGAAGCAAGAATTGATTACCATAAAAGGTAAGTATTTGA
CTTATCTAGATAAAGACTATTTTTTACAATACACAAAGTGA

Upstream 100 bases:

>100_bases
ATAGCAAAAAGAGGAGAAGGTGTAAAGTTTTTGATGAACCGCTTACAAAATGGCTTTTTTATGGTAAAATAAAGATGATG
TTTAGAAAAAGGAGATTTGT

Downstream 100 bases:

>100_bases
ATTAAAGGTCAGAACACTTTATTTTAAAAGTTTTTCTGACTTATTTTAATTTATAAGTATAAATTTTTACACTAAGATGT
AAAAAACATACAAGTAAACG

Product: hypothetical protein

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFFEGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHG
TIFPYGGLFTDDYYHFSVVAMTDVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDKDYFLQYTK

Sequences:

>Translated_226_residues
MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFFEGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHG
TIFPYGGLFTDDYYHFSVVAMTDVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDKDYFLQYTK
>Mature_226_residues
MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFFEGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHG
TIFPYGGLFTDDYYHFSVVAMTDVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDKDYFLQYTK

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26878; Mature: 26878

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFFEGDKRDKLFLVTSGYF
CCCCHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCEE
KVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMTDVTYFYFPVDLFEDYSLE
EEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCEEEEEEEEECEEEEEEEHHHHHCCCHH
NRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLAILLVEMGKDSDTLPFQLTTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECH
DIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDKDYFLQYTK
HHHHHCCCHHHHHHHHHHHHHHCCEEEEECCEEEEECCCCEEEECC
>Mature Secondary Structure
MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFFEGDKRDKLFLVTSGYF
CCCCHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCEE
KVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMTDVTYFYFPVDLFEDYSLE
EEEECCCCCCEEEEEHHHCCCCCCCCCCCCCCCEEEEEEEEECEEEEEEEHHHHHCCCHH
NRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLAILLVEMGKDSDTLPFQLTTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECH
DIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDKDYFLQYTK
HHHHHCCCHHHHHHHHHHHHHHCCEEEEECCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]