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The map label for this gene is rlmN

Identifier: 15675432

GI number: 15675432

Start: 1260865

End: 1261944

Strand: Reverse

Name: rlmN

Synonym: SPy_1533

Alternate gene names: 15675432

Gene position: 1261944-1260865 (Counterclockwise)

Preceding gene: 15675433

Following gene: 15675430

Centisome position: 68.12

GC content: 37.5

Gene sequence:

>1080_bases
ATGAAACCATCGATTTATAGCTTAACACGCGATGAACTGATCGCGTGGGCAGTTGAACGCGGCCAAAAACAGTTTAGAGC
TACTCAAATTTGGGATTGGCTCTATAAAAAAAGAGTTCAATCTTTTGAGGAGATGACGAATATTTCTAAAGATTTTGTTT
CTATTTTAAATGATAGTTTCTGTGTCAATCCTTTAAAACAGCGAGTTGTACAAGAGTCAGCTGATGGTACAGTAAAATAT
TTATTTGAACTGCCCGATGGTATGTTAATTGAAACAGTTTTAATGCGTCAACATTATGGTCATTCTGTTTGTGTGACGAC
TCAAGTTGGCTGTAACATTGGCTGTACTTTTTGTGCTAGTGGATTGATCAAAAAGCAACGTGATCTAAACAGTGGGGAAA
TTACAGCTCAAATCATGTTAGTCCAAAAATATTTTGACGATAGAAAGCAAGGTGAGCGGGTTAGCCATGTTGTCGTAATG
GGCATTGGTGAACCTTTTGATAATTACAAAAATGTCATGTGCTTTCTTCGCGTCATCAACGATGATAATGGCTTAGCAAT
TGGTGCCCGTCATATTACAGTCTCTACCTCAGGGTTAGCTCACAAGATTCGGGATTTTGCAAACGAGGGAGTACAGGTTA
ACTTAGCAGTCTCGTTGCATGCACCAAATAATGATTTACGTTCAAGTATTATGCGAGTAAATCGCTCTTTTCCGCTTGAA
AAGCTCTTTTCAGCTATAGAATACTATATTGAAAAAACGAATCGTCGTGTCACTTTTGAATACATTATGCTAAATGAAGT
TAACGATAGCATTAAGCAAGCTCAAGAATTAGCAGACTTAACTAAAACTATTCGTAAGTTATCTTATGTTAACTTGATTC
CGTATAATCCTGTTTCAGAACATGATCAGTATAGTAGAAGTCCTAAAGAGCGAGTTCTAGCGTTTTATGATGTGCTAAAG
AAAAATGGAGTTAACTGTGTGGTGCGTCAAGAGCACGGAACAGATATTGATGCAGCCTGTGGCCAGTTACGTTCTAAAAC
GATGAAAAAAGATCGCGAAAAGGTTACAGCGACAAAATAA

Upstream 100 bases:

>100_bases
AAGTAATACTAAGCATAAAGAAAAGTCAAAACGTAATCAGACTAGTCAACTTAATACCAAAATTAGTCATTTTATCATCA
GAAAGAAAGATAAATAATCC

Downstream 100 bases:

>100_bases
AGCCCAAAAAATATCTTTGATTGACAATGCTTGATGACAAAATATTCCTAGTCACTAAAATGACTAGGTTTTTTATCTGG
CCTAAAAATGCTCTAGAAGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK

Sequences:

>Translated_359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
>Mature_359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=35.8126721763085, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_STRP1 (Q99YU5)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269606.1
- RefSeq:   YP_282624.1
- ProteinModelPortal:   Q99YU5
- EnsemblBacteria:   EBSTRT00000001043
- EnsemblBacteria:   EBSTRT00000028092
- GeneID:   3571651
- GeneID:   901577
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1533
- KEGG:   spz:M5005_Spy_1261
- GeneTree:   EBGT00050000027236
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- ProtClustDB:   PRK14455
- BioCyc:   SPYO160490:SPY1533-MONOMER
- BioCyc:   SPYO293653:M5005_SPY1261-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41054; Mature: 41054

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVSILNDSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVMGIGEPFDNYKNVMCFLRVIN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHEEE
DDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
HDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVSILNDSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVMGIGEPFDNYKNVMCFLRVIN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHEEE
DDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
HDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296