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The map label for this gene is prfC [H]

Identifier: 15675335

GI number: 15675335

Start: 1175827

End: 1177371

Strand: Reverse

Name: prfC [H]

Synonym: SPy_1416

Alternate gene names: 15675335

Gene position: 1177371-1175827 (Counterclockwise)

Preceding gene: 15675336

Following gene: 15675334

Centisome position: 63.56

GC content: 39.87

Gene sequence:

>1545_bases
ATGTCACTTACAGAAGAAATTAAAAAACGCCGTACTTTTGCCATCATTTCTCACCCGGATGCTGGTAAGACGACTATTAC
TGAGCAACTATTATATTTTGGTGGTGAAATTAGAGAAGCAGGGACAGTAAAAGGCAAAAAATCAGGTACGTTTGCAAAGT
CCGACTGGATGGATATTGAAAAGCAACGTGGTATCTCTGTTACTTCATCTGTTATGCAATTTGATTACGCGGGTAAACGT
GTTAATATTTTGGATACACCTGGACATGAGGATTTCTCGGAAGATACATACCGTACCTTAATGGCTGTGGATGCGGCTGT
CATGGTTGTAGATTCGGCTAAAGGTATTGAGGCGCAAACGAAAAAACTTTTTGAAGTTGTCAAACATCGTAATATTCCTG
TCTTCACCTTTATTAACAAGTTAGACCGTGATGGGCGTGAGCCTTTAGAACTCTTGGAAGAATTAGAAGAAGTTTTAGGG
ATTGCTTCTTATCCGATGAACTGGCCGATTGGTATGGGACGCGCCTTTGAGGGCTTATATGATTTACATAATAAACGTTT
AGAACTCTATAAGGGAGACGAGCGTTTTGCAAGTATTGAAGACGGTGATCAGCTGTTTGCCAATAATCCCTTTTATGAAC
AAGTCAAAGAAGATATTGAACTTCTTCAAGAAGCAGGTAATGACTTTTCAGAACAAGCTATTCTTGATGGTGACCTAACT
CCAGTCTTTTTTGGCTCTGCCCTTACGAACTTTGGAGTTCAGACTTTTCTTGATACTTTCTTGGAGTTTGCCCCAGAACC
TCATGGTCACAAGACTACAGAAGGTAATGTTGTTGACCCCTTAGCTAAAGATTTTTCAGGTTTTGTCTTTAAAATTCAAG
CTAACATGGACCCTAAACACCGTGACCGTATTGCTTTTGTGCGTATTGTTTCTGGTGAATTTGAGCGCGGTATGGGAGTT
AATTTGACACGCACTGGCAAAGGCGCTAAGCTCTCCAATGTTACTCAATTTATGGCAGAATCACGGGAAAATGTGACGAA
TGCTGTTGCTGGGGATATTATCGGGGTTTATGATACAGGAACTTATCAAGTTGGGGATACCTTAACAGTTGGGAAAAATA
AATTTGAATTTGAACCCTTACCAACTTTTACCCCAGAAATCTTCATGAAGGTTTCTCCGAAAAATGTTATGAAGCAGAAA
TCCTTCCACAAAGGTATTGAACAATTAGTACAAGAAGGAGCAATTCAGCTTTATAAGAATTATCAGACAGGCGAATACAT
GCTGGGTGCTGTGGGTCAACTGCAGTTTGAAGTCTTTAAACATCGTATGGAAGGTGAATACAACGCTGAAGTTGTGATGA
CACCAATGGGTAAAAAGACTGTTCGCTGGATTTCAGAAGATGATTTGGATCAACGCATGTCTTCAAGCCGAAACATTTTA
GCTAAGGATCGTTTTGACCAACCAGTTTTCCTATTTGAAAATGATTTTGCCCTTCGCTGGTTTGCAGACAAATACCCAGA
TGTCACTTTAGAAGAGAAAATGTAA

Upstream 100 bases:

>100_bases
GAGAAACCCAATGTAGCCCAACTCTTTTGATAAACAAGTAATACCTAACGAAAATAACTCAGAGAAAAGCTCTGCCATTT
TACTATACAAGGAAAAAAGA

Downstream 100 bases:

>100_bases
TTGAAACATGATTTTCAACAGTCTAACGTTTTGGAGATCAATTTACTGAGCAACTTTTTAAAAGAAATGAGTGGCTAGTT
ATCTCTTTTGCAGATTTAAG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKEDIELLQEAGNDFSEQAILDGDLT
PVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGNVVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGV
NLTRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSPKNVMKQK
SFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVVMTPMGKKTVRWISEDDLDQRMSSSRNIL
AKDRFDQPVFLFENDFALRWFADKYPDVTLEEKM

Sequences:

>Translated_514_residues
MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKR
VNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKEDIELLQEAGNDFSEQAILDGDLT
PVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGNVVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGV
NLTRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSPKNVMKQK
SFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVVMTPMGKKTVRWISEDDLDQRMSSSRNIL
AKDRFDQPVFLFENDFALRWFADKYPDVTLEEKM
>Mature_513_residues
SLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRV
NILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGI
ASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKEDIELLQEAGNDFSEQAILDGDLTP
VFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGNVVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVN
LTRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSPKNVMKQKS
FHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVVMTPMGKKTVRWISEDDLDQRMSSSRNILA
KDRFDQPVFLFENDFALRWFADKYPDVTLEEKM

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=480, Percent_Identity=30.2083333333333, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI19923640, Length=551, Percent_Identity=26.6787658802178, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=29.873417721519, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=33.448275862069, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=33.1210191082803, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=30.6569343065693, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=46.9230769230769, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789738, Length=492, Percent_Identity=27.6422764227642, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=31.875, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=30.7262569832402, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=502, Percent_Identity=28.2868525896414, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17556745, Length=475, Percent_Identity=25.8947368421053, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=32.8467153284672, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=171, Percent_Identity=28.6549707602339, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=490, Percent_Identity=28.1632653061224, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=27.1844660194175, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=28.4810126582279, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=28.4810126582279, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=28.4823284823285, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI221458488, Length=514, Percent_Identity=25.875486381323, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585709, Length=173, Percent_Identity=29.4797687861272, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585711, Length=173, Percent_Identity=29.4797687861272, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585713, Length=173, Percent_Identity=29.4797687861272, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR002127
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58272; Mature: 58141

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGTFAKSDWMDIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHH
KKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGIASYPMNWPIGMGRAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKEDIELLQEAGNDFSEQAILDGDLT
HHHCCCEEEEECCCCEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHEEECCCCC
PVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGNVVDPLAKDFSGFVFKIQANMDPKH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSPKNVMKQKSFHKGIEQLVQEGAIQLYKN
CEECCCEEEECCCCEECCCCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVVMTPMGKKTVRWISEDDLDQRMSSSRNIL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCCCHHHHHHHCCCEE
AKDRFDQPVFLFENDFALRWFADKYPDVTLEEKM
ECCCCCCCEEEEECCEEEEEECCCCCCCEECCCC
>Mature Secondary Structure 
SLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGTFAKSDWMDIE
CHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHH
KKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGIASYPMNWPIGMGRAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKEDIELLQEAGNDFSEQAILDGDLT
HHHCCCEEEEECCCCEECCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHEEECCCCC
PVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGNVVDPLAKDFSGFVFKIQANMDPKH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNVTQFMAESRENVTNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVSPKNVMKQKSFHKGIEQLVQEGAIQLYKN
CEECCCEEEECCCCEECCCCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVVMTPMGKKTVRWISEDDLDQRMSSSRNIL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCCCHHHHHHHCCCEE
AKDRFDQPVFLFENDFALRWFADKYPDVTLEEKM
ECCCCCCCEEEEECCEEEEEECCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA