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The map label for this gene is ytzG [H]

Identifier: 15675319

GI number: 15675319

Start: 1159756

End: 1160340

Strand: Reverse

Name: ytzG [H]

Synonym: SPy_1398

Alternate gene names: 15675319

Gene position: 1160340-1159756 (Counterclockwise)

Preceding gene: 15675320

Following gene: 15675318

Centisome position: 62.64

GC content: 41.03

Gene sequence:

>585_bases
ATGCGTTTAGATAAATTATTAGAAGGTACAAAAGTTGGCTCTAGAAGTCAAGTGAAGAAACTGATTAAGGCTCAAGGGGT
TTGGGTTGATCACATGCCTGCCAGAAATGGTCGACAAAATGTTGATCCTGGCTTGCAACTGATTGAGGTGACTGGACAGC
GAGTGACGCATCCTAAACATTCTTACATTATCCTCAACAAGCCATCAGGGGTGGTCTCAGCTAAGAAAGATACTAACTAT
TTAACCGTTATTGATCAGCTTGCTGAAGAAGATAAAAGTCCAGACTTGTATCCTGTTGGCAGATTGGATAGAGATACCGA
AGGGTTGGTTTTACTCACTGATAATGGTCCCCTAGGGTTTCGGATGTTGCACCCTAGTCATCATGTTTCTAAAACGTATC
TTGTAACCGTTAATGGCTTGTTAGCAGAAGATGCCTCAGATTTTTTTGCAGCTGGTATTTGTTTCCCAACAGGTGAACAA
TGCCAACCAGCTCAATTGACTATTCTTAAAGCCGATACAGACCAGTCACAGGCTAGTTTGACCATTTCAGAAGGAAAATT
TCACCAAGTGAAAAAATGTTTTTGA

Upstream 100 bases:

>100_bases
CTTGTAAGAACAAAAATGAGGAGATAGTCAACTTCATTTAAGAAGAAGTCTTGTAACTTCTATTTCTTTTGCAAGGGTAA
ATTTATGTTACACTACTAAT

Downstream 100 bases:

>100_bases
CCTATGGTTTGAAAGTGACCTCTCTGAAACGGACGCACTTTGCAGGACTGGAACTCGGTCATTTAGCCAGCGGAGAATAT
CGTTATCTAACAGCGTCAGA

Product: putative 16S pseudouridylate synthetase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKCF

Sequences:

>Translated_194_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKCF
>Mature_194_residues
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNY
LTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
CQPAQLTILKADTDQSQASLTISEGKFHQVKKCF

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=202, Percent_Identity=36.1386138613861, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790453, Length=157, Percent_Identity=31.8471337579618, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1787524, Length=203, Percent_Identity=29.064039408867, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 21391; Mature: 21391

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKH
CCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCC
SYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGF
CEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASL
EEECCCCCCCEEEEEEECCEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
TISEGKFHQVKKCF
EECCCCHHHHHHCC
>Mature Secondary Structure
MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPGLQLIEVTGQRVTHPKH
CCHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCC
SYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRLDRDTEGLVLLTDNGPLGF
CEEEEECCCCCEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCEEE
RMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQCQPAQLTILKADTDQSQASL
EEECCCCCCCEEEEEEECCEECCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
TISEGKFHQVKKCF
EECCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]