Click here to switch to the map view.

The map label for this gene is metK [H]

Identifier: 15675292

GI number: 15675292

Start: 1125330

End: 1126526

Strand: Reverse

Name: metK [H]

Synonym: SPy_1359

Alternate gene names: 15675292

Gene position: 1126526-1125330 (Counterclockwise)

Preceding gene: 15675293

Following gene: 15675291

Centisome position: 60.81

GC content: 42.86

Gene sequence:

>1197_bases
ATGTCAGAACGTAAACTTTTCACGTCTGAGTCCGTATCTGAAGGGCATCCAGATAAGATTGCAGACCAAATTTCAGATGC
TATTTTAGATGCCATTTTAGCAGAAGATCCAGAAGCCCATGTGGCTGCAGAAACTTGTGTTTACACAGGTTCTGTTCATG
TTTTTGGAGAAATCTCAACAACAGCATACATTGATATTAACCGTGTGGTCCGCGACACCATTGCAGAAATTGGTTATACA
GAAGCAGAATATGGCTTTTCAGCAGAATCAGTGGGAGTACATCCATCACTTGTAGAACAATCTGGAGATATCGCTCAGGG
GGTTAATGAAGCTTTCGAAAGCAGAGAAGGTGATACTGATGACCTTAGCCATATTGGAGCGGGTGACCAAGGGCTCATGT
TTGGTTTTGCCATCAATGAAACACCAGAATTGATGCCTTTACCAATTTCCTTATCTCACCAATTAGTGCGTCGCTTGGCG
GAATTACGTAAGTCAGGTGAGATTAGTTATCTACGTCCAGATGCTAAATCTCAAGTGACAGTTGAGTACGATGAACACGA
TAAACCTGTTCGTGTGGATACAGTCGTTATTTCTACTCAGCATGATCCAGAAGCTACTAATGACCAAATTCGTCAGGATG
TGATTGAAAAAGTGATCAAGGCTGTAATTCCAGCAGATTATTTAGATGATGACACAAAGTTCTTCATTAATCCAACAGGA
CGTTTTGTTATTGGAGGTCCGCAAGGAGACTCTGGATTGACCGGACGTAAGATTATTGTGGATACCTATGGAGGCTATTC
ACGTCATGGTGGCGGTGCTTTTTCAGGAAAAGATGCGACAAAAGTGGACCGTTCGGCTTCTTACGCTGCTCGCTATATTG
CTAAAAACCTTGTGGCAGCAGGCCTTGTGACCAAAGCTGAAGTCCAACTAGCTTATGCGATTGGGGTGGCTCAGCCTGTG
TCTGTTCGCGTGGATACATTTGGAACCTCAACGGTTCCTGAAGCTGTTTTAGAAGCTGCTGTTCGTCAAGTCTTTGATTT
ACGGCCAGCAGGCATTATTCAAATGCTTGATTTAAAACGCCCGATTTACAAACAAACAGCTGCCTATGGTCACATGGGGA
GAACAGATATTGATTTGCCATGGGAGCGTTTAAATAAGGTTGATGCTTTAGTAGAAGCTGTAAAAACAGTATTATAA

Upstream 100 bases:

>100_bases
ACTTGCTCAATCAGCTAGGAAGAAAGGGGTAGGCAACTTCTTGCTTTGTGGTCAATGTGACTGAAAAAGGAAGAATCAAC
ATATTAGGGGGGACATTTTT

Downstream 100 bases:

>100_bases
ACGACAACTTTATTAAAAAAGTAGAGTAAGTGAAAAAATCTTACTCTGCTTTTTTGTTATTAAATAAGCCTTTAAAAGGG
ATGATTAAAAATTAAGACGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAETCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYT
EAEYGFSAESVGVHPSLVEQSGDIAQGVNEAFESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKVIKAVIPADYLDDDTKFFINPTG
RFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLVTKAEVQLAYAIGVAQPV
SVRVDTFGTSTVPEAVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTVL

Sequences:

>Translated_398_residues
MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAETCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYT
EAEYGFSAESVGVHPSLVEQSGDIAQGVNEAFESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKVIKAVIPADYLDDDTKFFINPTG
RFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLVTKAEVQLAYAIGVAQPV
SVRVDTFGTSTVPEAVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTVL
>Mature_397_residues
SERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAETCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTE
AEYGFSAESVGVHPSLVEQSGDIAQGVNEAFESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLAE
LRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKVIKAVIPADYLDDDTKFFINPTGR
FVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGLVTKAEVQLAYAIGVAQPVS
VRVDTFGTSTVPEAVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTVL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=56.3157894736842, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI5174529, Length=386, Percent_Identity=56.2176165803109, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=60.5128205128205, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17538494, Length=401, Percent_Identity=55.1122194513716, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17538498, Length=387, Percent_Identity=56.5891472868217, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=55.2429667519182, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=52.3690773067332, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565905, Length=401, Percent_Identity=51.1221945137157, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=54.6268656716418, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=339, Percent_Identity=52.8023598820059, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI32565903, Length=323, Percent_Identity=53.8699690402477, Blast_Score=317, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI32565909, Length=99, Percent_Identity=51.5151515151515, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=55.440414507772, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=52.9262086513995, Blast_Score=396, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=51.5789473684211, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=51.5789473684211, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=52.3684210526316, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580541, Length=382, Percent_Identity=49.2146596858639, Blast_Score=334, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=47.2, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43180; Mature: 43049

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAETCVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEEECE
TAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSGDIAQGVNEAFESREGDTD
EEEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCH
DLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLAELRKSGEISYLRPDAKSQVT
HHHHCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEE
VEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKVIKAVIPADYLDDDTKFFINPTG
EEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
RFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNLVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLVTKAEVQLAYAIGVAQPVSVRVDTFGTSTVPEAVLEAAVRQVFDLRPAGIIQMLDLKR
HHHHHHHEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
PIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTVL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAETCVYTGSVHVFGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEEECE
TAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSGDIAQGVNEAFESREGDTD
EEEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCH
DLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLAELRKSGEISYLRPDAKSQVT
HHHHCCCCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEE
VEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKVIKAVIPADYLDDDTKFFINPTG
EEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
RFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKDATKVDRSASYAARYIAKNLVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLVTKAEVQLAYAIGVAQPVSVRVDTFGTSTVPEAVLEAAVRQVFDLRPAGIIQMLDLKR
HHHHHHHEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
PIYKQTAAYGHMGRTDIDLPWERLNKVDALVEAVKTVL
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA