Click here to switch to the map view.

The map label for this gene is amyB [C]

Identifier: 15675255

GI number: 15675255

Start: 1081754

End: 1083457

Strand: Reverse

Name: amyB [C]

Synonym: SPy_1304

Alternate gene names: 15675255

Gene position: 1083457-1081754 (Counterclockwise)

Preceding gene: 15675256

Following gene: 15675254

Centisome position: 58.49

GC content: 38.2

Gene sequence:

>1704_bases
ATGAATGTTGCCGGAATGCTCCATATTCCTGATTCGAGGTATTGCTTTGCCATATCTCAAAAGGAACTTGTCATCCGATT
ACGCGTGGCCAAAGAAGATAGAGGTATTACTGTTAATTTGGTCCACGGGCCTAAATATGGTTATCATGAATTCCAAGAAG
AATTGGAAATGTCAGTTGCCTATACAGACCATACGCATGCTTATTATGAAACTACTTTAACATTAGACGATGTGAGGTTG
GCCTACATTTTTAAAATTGAAGAAGAAGGTCAAACCTATTATTTTAGTGAAGATGGGTTAACTACTAGTTACGATTTTTC
AAATGGGTTTTATAATTTCTTCCAAATGCCTTATATCAATGCTGTTGATGTTCATCAAGTCATTCCTTGGACAAAAGACG
CGGTTTTTTATCAAATCTTTGTTGATCGCTTTAACCGTGGGGATTGGGAAAAAGGTGACAGTTATGTCAACATGGGTTGG
TTAGAAAAACCGACTCCTAAAAGTTTTGCTGGTGGAGATTTAAAGGGAATTACAGAAAAATTAGATTACCTCAAAGATTT
AGGGATTACTGTCATCTATTTAACGCCAATTTTTCAATCCATTAGTAATCACAAATATGATATTAGTGATTACTACGCTA
TTGATCCACAATTTGGAACTAAATATGATTTGCAAGAATTGATTGATTTAGCTCACAAAATGGGAATCAAGATTATTCTA
GACGCTGTCTTTAACCATGCCAGCAGTGATGCCGTGGAATTTCAGGATGTCTTGAGATACGGTAAAGAGTCTAAGTTTTT
TGATTGGTTTATGACTCATGATGAACACCCATCCATGGACCTAGTAAACTATGAAACCTTTGCTGGATGTAACTATATGC
CCAAATGGAATACCTCAAATAGAGACGTGCAAGACTATCTGATTGAGATTGGGCGATACTGGATTAAAGAATTTTGTATC
GATGGTTGGCGCCTAGATGTTTCTGATGAAGTATCTCATGATTTTTGGCGACGTTTCCGTCAGGCGGTTAAAGCGGAAAA
AGCAGATGCCATTTTAATTGGGGAGAACTGGCATGATGCCTATCCTTATTTGGCAGGGGATCAGTATGATGGGATAATGA
ATTATGCTTTCACCAAAGCCTGTTTGGATTACTTTGCTTTTGAAACCATGACATCAAAACAATTGGCTGAGAAATTAAGC
AACATTTTGATGCGAAACACTTGGCAAGTCAATCGCATGAACTTGAATCTTTTGGATTCTCATGATACGCATCGTTTCTT
CACTCAAGTTAAGGGTTCCAAAGATAAATTGTTAGCTGCTTTAGCATTGCTCTTTATCTTTATGGGCATTCCTTGCTTGT
ATTATGGGACAGAAGTGGCAATGGAAGGGGGTTACGACCCTGATTCGCGTCGCGGTTTTCCTTGGGATGATGCTGAGCGG
GACAAGGATTTCTTTAGCAAAGTAAAAGCGATTATTGCTCTTCGCCAACAGCCAGCTCTTCAAGAAGGAGCTATTAGCAT
TGATTATGACGAAGACTGCCTCATTCTGAAACGCAGCTTAGGTGAGCAAAGCATTTCACTTGTTCTTAACAATGGTGAGA
GTGCTTATTCTATTAAGGAAGGAGGACAGATTTTGACAAGCAATGGGGTAACTAAAAACAAAAAACAATTACTCCAGGAT
GGATATATTGTGTTTAGTAACTAA

Upstream 100 bases:

>100_bases
TGAAACAGATACTAAAAATATAGAAGTATGAGTCGCTCTGCTATTTTTTCGTTTTAAAGAAAAAGAAGTAATCGTCAATA
CTTTATTAATGGAGGTAGAA

Downstream 100 bases:

>100_bases
AAGGTTATTTTAAAAGGAAAAAGCGATGAGAGAATTACATATCAAAACTTATAAGTTATTAACGAAAAGTGCTGTTTTAC
TTGGCTTAATTTCATTTCCA

Product: putative cyclomaltodextrinase

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRL
AYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL
DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCI
DGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLS
NILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWDDAER
DKDFFSKVKAIIALRQQPALQEGAISIDYDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQD
GYIVFSN

Sequences:

>Translated_567_residues
MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRL
AYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL
DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCI
DGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLS
NILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWDDAER
DKDFFSKVKAIIALRQQPALQEGAISIDYDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQD
GYIVFSN
>Mature_567_residues
MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRL
AYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL
DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCI
DGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLS
NILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWDDAER
DKDFFSKVKAIIALRQQPALQEGAISIDYDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQD
GYIVFSN

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=199, Percent_Identity=31.1557788944724, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI189458812, Length=148, Percent_Identity=30.4054054054054, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1786604, Length=521, Percent_Identity=30.9021113243762, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790687, Length=196, Percent_Identity=34.1836734693878, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789995, Length=499, Percent_Identity=24.0480961923848, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=143, Percent_Identity=39.1608391608392, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=143, Percent_Identity=39.1608391608392, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321726, Length=260, Percent_Identity=27.6923076923077, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322241, Length=225, Percent_Identity=30.2222222222222, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322021, Length=225, Percent_Identity=30.2222222222222, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324416, Length=263, Percent_Identity=29.277566539924, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322245, Length=225, Percent_Identity=31.1111111111111, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320826, Length=171, Percent_Identity=29.8245614035088, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24586591, Length=230, Percent_Identity=31.7391304347826, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24583747, Length=153, Percent_Identity=41.1764705882353, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24583749, Length=153, Percent_Identity=41.1764705882353, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24586587, Length=407, Percent_Identity=25.3071253071253, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI221330053, Length=146, Percent_Identity=40.4109589041096, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24586599, Length=225, Percent_Identity=31.5555555555556, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24586593, Length=227, Percent_Identity=29.9559471365639, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24586597, Length=146, Percent_Identity=39.7260273972603, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45549022, Length=133, Percent_Identity=40.6015037593985, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24586589, Length=229, Percent_Identity=29.6943231441048, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583745, Length=142, Percent_Identity=37.3239436619718, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 65690; Mature: 65690

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVA
CCCCEEEECCCCCEEEEECCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHEEEE
YTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYIN
EECCCCEEEEEEEEECCEEEEEEEEEECCCCEEEEECCCCEEEEECCCCCHHHHCCCCCC
AVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGWLEKPTPKSFAGGDLKGITEK
CCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHH
LDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL
HHHHHHCCCEEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHH
DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMDLVNYETFAGCNYMPKWNTSN
HHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCCCCCCC
RDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHDA
CCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNLNLLDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECC
HDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWDDAER
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
DKDFFSKVKAIIALRQQPALQEGAISIDYDEDCLILKRSLGEQSISLVLNNGESAYSIKE
HHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCHHHHHHHCCCCEEEEEEECCCCEEEECC
GGQILTSNGVTKNKKQLLQDGYIVFSN
CCEEEECCCCCHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVA
CCCCEEEECCCCCEEEEECCCEEEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHEEEE
YTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYIN
EECCCCEEEEEEEEECCEEEEEEEEEECCCCEEEEECCCCEEEEECCCCCHHHHCCCCCC
AVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGWLEKPTPKSFAGGDLKGITEK
CCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHH
LDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL
HHHHHHCCCEEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHH
DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMDLVNYETFAGCNYMPKWNTSN
HHHHCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEECCHHHCCCCCCCCCCCCC
RDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHDA
CCHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNLNLLDS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECC
HDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWDDAER
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
DKDFFSKVKAIIALRQQPALQEGAISIDYDEDCLILKRSLGEQSISLVLNNGESAYSIKE
HHHHHHHHHHHHHHHCCCCHHHCCEEECCCCCHHHHHHHCCCCEEEEEEECCCCEEEECC
GGQILTSNGVTKNKKQLLQDGYIVFSN
CCEEEECCCCCHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]