Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 15675225

GI number: 15675225

Start: 1043809

End: 1046127

Strand: Reverse

Name: pcrA [H]

Synonym: SPy_1267

Alternate gene names: 15675225

Gene position: 1046127-1043809 (Counterclockwise)

Preceding gene: 15675228

Following gene: 15675224

Centisome position: 56.47

GC content: 41.1

Gene sequence:

>2319_bases
ATGAATCCTTTATTAAATGGAATGAATGATCGGCAGGCACAAGCTGTTCAAACAACTGAAGGCCCCTTATTGATTATGGC
GGGGGCTGGTTCTGGTAAAACAAGGGTACTGACCCATCGTATTGCCTATTTAATTGATGAAAAATTTGTCAATCCTTGGA
ATATTTTAGCTATTACCTTTACTAATAAGGCTGCGCGTGAAATGAAAGAAAGGGCCTTGGCCTTAAATCCAGCTACTAAA
GATACCTTAATTGCTACCTTTCACTCCATGTGTGTACGGATTCTGCGTCGTGAGGCTGATCACATTGGTTACAACCGAAA
CTTCACAATCGTGGATCCAGGTGAGCAACGAACGTTGATGAAGCGCATTTTAAAACAATTAAATATAGACCCTAAAAAGT
GGAATGAACGTTCTATTTTAGGAACTATTTCAAACGCTAAAAATGATTTGCTTGATGAAAAGGGATACGAGGCACAGGCT
GCTGATATGTATAGTCAAATCGTAGCGAGGTGTTACAAAGCTTATCAGGAAGAATTACGACGTAGCGAAGCACTTGACTT
TGATGATTTAATCATGATGACGCTACGCCTGTTTGACGCTAATCCTGACGTGTTAGCTTATTACCAGCAGCGTTACCAAT
ACATTCATGTGGATGAGTACCAAGATACCAACCATGCTCAATATCAGCTAATTAAATTACTAGCTTCTCGCTTTAAAAAT
ATTTGCGTTGTTGGAGACGCTGATCAGTCTATCTATGGTTGGCGGGGAGCTGACATGCAAAATATTCTTGATTTTGAAAA
AGATTACCCAGATGCTAAGGTAGTGCTTTTAGAAGAAAACTATCGCTCTACCAAGAAAATTCTGCAAGCAGCTAACGACG
TGATTAACAATAACCGTAATCGCCGTCCTAAGAAACTTTGGACACAAAATGCGGATGGTGAACAACTGGTTTATTACCGG
GCAAATGATGAGCGAGATGAGGCAGTATTTGTGGCCTCAACCATTAGCAATATGAGTCAAGAGCTTGGAAAAAACTTCAA
AGATTTCGCTGTTTTATACCGTACCAATGCCCAATCTCGGACCATTGAAGAAGCACTCTTAAAATCCAATATTCCTTACA
CCATGGTTGGAGGCACCAAGTTTTACAGCCGTAAAGAAATTCGGGATTTGATTGCCTATTTAACCATTGTTGCTAACCCT
GCTGATAATATTTCTTTTGAGCGGATTGTGAATGAGCCTAAACGAGGTGTTGGTCCAGGAACGCTTGATAAACTGCGTCA
GTTTGCCTATGAGAGTGACCAGTCTTTATTAGAAGCAGCTTCAAATCTATTGATGTCCCCCTTGAAAGGTAAAGCAGCAC
AAGCTATCATGGACTTGGCCAATATTCTCGGTCAGCTACGTCAAGACCTAGATCAGATGAGTATCACCGACTTAGCAGAA
GCTTTGTTAGAAAAAACAGGTTATTTAGATAGCTTACGCCTGCAAAATACATTGGAAAGCCAAGCCCGTATCGAAAATAT
CGAAGAATTCTTATCTGTTACCAAAAATTTTGACGAGTCAAGTGCCTCTCAAGAAGAAGACGAAACCGGTGTTGATCGCT
TGGGACGTTTTTTAAACGATTTAGCACTGATTGCTGATACAGATGATTCCCAAGCAGAAGCTGCTGAAGTAACCTTAATG
ACCCTACATGCTGCCAAAGGCTTGGAGTTCCCTGTTGTTTTCTTAATTGGTATGGAAGAAGGTGTCTTCCCCTTATCACG
TGCCTCTGAAGATCCAGATGAATTGGAAGAAGAAAGACGTTTAGCTTATGTCGGAATCACAAGAGCTGAAGAAGTTCTCT
TTATGACCAATGCCAACACCAGAACCCTCTTTGGTAAAAGTAGTTATAACCGTCCAACACGCTTCTTGAAAGAAATTTCA
GAGGAACTACTCTCTTATAAAGGATTGGCAAGACCAGCACAATCTTCATTTGGTGTGCGTTTTTCTACTGAAACACACAC
ACAGTTTGGACAAGGGATGAGCCTATCAGAAGCCTTACAAGCTCGAAAGGCCCAGGCTCAAGTAAGACAATCAGCACAGC
CAATGCAGGCACATACGATACCATCAGCTTCAACTAGCTCAGTTTTACCGTTTGGTTCTAATAGCTCTGTAGAAGAGGTC
ACTTGGCAGATTGGCGATATCGCTCACCATAAAAAATGGGGAGACGGGACTGTGCTTGAGGTTTCAGGCTCCGGAAAAAC
GATGGAATTGAAAATCAAATTTCCAGAAGTTGGTTTAAAGAAATTACTAGCAAGTGTTGCTCCTATTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
CTCTTTACTTTTGGGGTCTTCTTTGCTATTTTTCTTGGAAACATGATATAATTTTAAGGTTGATAAAAGAGATGTCACTG
AACGAGATTAAGGAGAAAGC

Downstream 100 bases:

>100_bases
GAAAAAAATGAGCTTCCTACTATATAAGTGAGTAGGAAGCTCATTTTTTTTAGATTTCTGTATCATATAAAAGTTAAAAA
ATTTTAAGTTTTAGGAGCAT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 772; Mature: 772

Protein sequence:

>772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLIKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK

Sequences:

>Translated_772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLIKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
>Mature_772_residues
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITFTNKAAREMKERALALNPATK
DTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLIKLLASRFKN
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYR
ANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANP
ADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLM
TLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEIS
EELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEV
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=776, Percent_Identity=38.659793814433, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI48994965, Length=653, Percent_Identity=39.9693721286371, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=29.041095890411, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324477, Length=669, Percent_Identity=23.4678624813154, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 87032; Mature: 87032

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITF
CCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQAADMYSQIVARCYKAYQEELR
HHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLIKLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRN
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
RRPKKLWTQNADGEQLVYYRANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHEEEEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANPADNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCC
TLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LALIADTDDSQAEAAEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERR
HEEEECCCCCCHHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHC
LAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVR
EEEEEECCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEV
EECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHH
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKFVNPWNILAITF
CCCCCCCCCCHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAREMKERALALNPATKDTLIATFHSMCVRILRREADHIGYNRNFTIVDPGEQRTLM
CCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
KRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQAADMYSQIVARCYKAYQEELR
HHHHHHCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVDEYQDTNHAQYQLIKLLASRFKN
HHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCC
ICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVLLEENYRSTKKILQAANDVINNNRN
EEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC
RRPKKLWTQNADGEQLVYYRANDERDEAVFVASTISNMSQELGKNFKDFAVLYRTNAQSR
CCCHHHHCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHCCCHHHHEEEEECCCCHH
TIEEALLKSNIPYTMVGGTKFYSRKEIRDLIAYLTIVANPADNISFERIVNEPKRGVGPG
HHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCC
TLDKLRQFAYESDQSLLEAASNLLMSPLKGKAAQAIMDLANILGQLRQDLDQMSITDLAE
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLEKTGYLDSLRLQNTLESQARIENIEEFLSVTKNFDESSASQEEDETGVDRLGRFLND
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
LALIADTDDSQAEAAEVTLMTLHAAKGLEFPVVFLIGMEEGVFPLSRASEDPDELEEERR
HEEEECCCCCCHHHHHEEEEEEEHHCCCCCCEEEEEECCCCCCCHHCCCCCHHHHHHHHC
LAYVGITRAEEVLFMTNANTRTLFGKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVR
EEEEEECCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
FSTETHTQFGQGMSLSEALQARKAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEV
EECCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCHHHH
TWQIGDIAHHKKWGDGTVLEVSGSGKTMELKIKFPEVGLKKLLASVAPIEKK
HHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA