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The map label for this gene is oadA [H]

Identifier: 15675160

GI number: 15675160

Start: 976890

End: 978299

Strand: Direct

Name: oadA [H]

Synonym: SPy_1191

Alternate gene names: 15675160

Gene position: 976890-978299 (Clockwise)

Preceding gene: 15675159

Following gene: 15675164

Centisome position: 52.74

GC content: 42.55

Gene sequence:

>1410_bases
ATGAGGAGAACAATTATGACGAAAATCCGTATAACGGAAACAGTTTTACGTGATGGTCAACAAAGTCAGATTGCCACACG
TATGACAACCAAGGAAATGATTCCTATTCTCGAAACGCTAGACAATGCTGGTTATCATGCCCTTGAAATGTGGGGAGGAG
CAACCTTTGATTCCTGCCTACGCTTTTTAAATGAAGACCCTTGGGAACGGCTAAGAGCCATCCGTAAAGCAGTGAAAAAG
ACTAAACTTCAGATGCTCTTACGCGGACAAAACCTTCTTGGATATCGCAATTATGCTGACGATGTGGTCAGATCCTTTAT
TCAAAAATCCATTGAAAATGGGATTGATATTGTCCGTATTTTCGATGCTTTAAACGACCCACGCAACTTGCAAACAGCTG
TTTCAGCGACTAAAAAATTTGGAGGGCATGCTCAAGTTGCCATTTCTTACACGACAAGTCCGGTACATACCATTGACTAC
TTTGTTGAATTGGCGAAAGCTTACCAAGCTATCGGAGCGGACTCCATTTGTATCAAAGACATGGCTGGTGTCTTAACTCC
TGAAATAGGTTACCAATTGGTCAAATGTATCAAGGAGAATACAACTATCCCTCTTGAGGTTCATACCCATGCTACCAGTG
GTATTTCAGAAATGACTTACTTAAAAGTAGCAGAAGCAGGAGCTGATATTATTGATACGGCGATTTCCTCTTTTTCAGGG
GGAACCAGTCAGCCTGCCACAGAGTCAATGGCGATTGCCTTGACGGATTTAGGCTTTGACACAGGCTTGGATATGCAAGA
GGTGGCCAAAGTTGCAGAATATTTCAACACTATTCGTGACCACTATCGAGAAATAGGGATTTTAAATCCTAAAGTCAAAG
ATACTGAGCCAAAAACATTGATTTACCAAGTTCCAGGTGGCATGCTATCAAACCTATTGAGCCAATTAACCGAGCAAGGC
CTAACTGATAAATACGAAGAAGTCTTAGCAGAAGTACCTAAAGTAAGGGCTGATCTTGGGTATCCGCCACTCGTAACGCC
ATTATCACAAATGGTTGGCACACAGGCCTTGATGAATATCATCTCAGGGGAACGTTACAAGGTAGTTCCAAATGAAATCA
AAGACTATGTTCGAGGGCTATACGGTCAATCACCAGCGCCATTAGCAGAAGGTATCAAAGAGAAAATCATTGGTGACGAA
GCAGTTATTACTTGCAGACCAGCCGACCTAATCGAGCCTCAAATGATTTATCTACGTGACGAGATTGCTCCATACGCTCA
TTCAGAAGAAGACGTGTTAAGCTATGCAAGCTTCCCGCAACAAGCTAGAGATTTCTTGGGACGCCGTGAAGATCCTTTCT
ATGATGTTCCGGTTCAAGAAGTTACTGTACAACTGGACATTCAAGACTAA

Upstream 100 bases:

>100_bases
GTAAGCACAGTGTCGAGGAGATGCAGACTGCTATTTCAAAATTACTCCATTCATTTTTCAATAAAGACAACCAATCATCG
TCATCAGATAAGACAGGTTG

Downstream 100 bases:

>100_bases
CAGAGAATGACTAATCCTCTTAACCATTAATCTCCTTGGTGAAACGCATTTGCTGTAAGAACAATACCAAAAACCTACCT
GATTTTGATAATCAGATAGG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRRTIMTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKK
TKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDY
FVELAKAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSG
GTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQG
LTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDE
AVITCRPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD

Sequences:

>Translated_469_residues
MRRTIMTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKK
TKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDY
FVELAKAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSG
GTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQG
LTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDE
AVITCRPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
>Mature_469_residues
MRRTIMTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCLRFLNEDPWERLRAIRKAVKK
TKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDY
FVELAKAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSG
GTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQG
LTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDE
AVITCRPADLIEPQMIYLRDEIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI106049295, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI106049292, Length=451, Percent_Identity=32.3725055432373, Blast_Score=228, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17562816, Length=483, Percent_Identity=33.5403726708075, Blast_Score=254, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6321376, Length=461, Percent_Identity=33.1887201735358, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6319695, Length=461, Percent_Identity=32.9718004338395, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI281363050, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652224, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652222, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652220, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652218, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652212, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652210, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652214, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921944, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24652216, Length=461, Percent_Identity=34.70715835141, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52268; Mature: 52268

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTIMTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHH
RFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
FDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELAKAYQAIGADSICIKD
HHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSG
HHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCC
GTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTL
CCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IYQVPGGMLSNLLSQLTEQGLTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNI
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITCRPADLIEPQMIYLRD
HCCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHHHH
EIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
HHCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEECC
>Mature Secondary Structure
MRRTIMTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALEMWGGATFDSCL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHH
RFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFIQKSIENGIDIVRI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
FDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELAKAYQAIGADSICIKD
HHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKVAEAGADIIDTAISSFSG
HHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCC
GTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDHYREIGILNPKVKDTEPKTL
CCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
IYQVPGGMLSNLLSQLTEQGLTDKYEEVLAEVPKVRADLGYPPLVTPLSQMVGTQALMNI
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKIIGDEAVITCRPADLIEPQMIYLRD
HCCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCHHHCCCHHHHHHH
EIAPYAHSEEDVLSYASFPQQARDFLGRREDPFYDVPVQEVTVQLDIQD
HHCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]