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The map label for this gene is rnhB

Identifier: 15675138

GI number: 15675138

Start: 954926

End: 955717

Strand: Direct

Name: rnhB

Synonym: SPy_1162

Alternate gene names: 15675138

Gene position: 954926-955717 (Clockwise)

Preceding gene: 15675137

Following gene: 15675139

Centisome position: 51.55

GC content: 40.53

Gene sequence:

>792_bases
ATGCCGACTAGTATTAAAGCTATTAAAGAAAGCTTAGAGGCCGTTACTAGCCTCTTGGACCCCCTCTTTCAAGAATTGGC
AACCGACACTAGGTCAGGCGTCCAAAAAGCTCTAAAAAGCCGACAAAAGGTTATTCAGGCCGAGTTAGCAGAAGAAGAAC
GATTAGAAGCCATGCTTTCTTATGAAAAAGCTCTTTATAAAAAAGGTTATAAAGCCATTGCAGGTATTGATGAGGTGGGA
CGTGGTCCCTTAGCAGGTCCCGTTGTGGCAGCTTGTGTGATTTTACCTAAGTATTGTAAAATTAAAGGCCTTAATGATTC
TAAAAAAATCCCTAAAGCTAAGCATGAGACCATTTATCAGGCAGTGAAAGAAAAGGCTTTGGCTATCGGTATCGGTATTA
TTGACAATCAGCTTATTGATGAGGTCAATATTTATGAAGCAACCAAACTGGCCATGCTAGAAGCCATTAAACAGTTGGAG
GGCCAACTCACACAACCAGATTATCTCTTGATTGATGCCATGACATTGGATATTGCTATTTCGCAGCAGTCTATTCTTAA
AGGCGATGCCAATTCCTTGTCTATTGCAGCAGCATCAATTGTAGCTAAGGTCACCAGAGATCAGATGATGGCTAACTATG
ATCGCATTTTTCCTGGTTATGACTTTGCTAAAAATGCAGGCTATGGCACCAAAGAACATTTACAGGGATTAAAAGCTTAC
GGCATAACGCCTATCCATCGTAAAAGTTTTGAACCTGTTAAATCCATGTGCTGCGATTCAACTAATCCTTAA

Upstream 100 bases:

>100_bases
GGATTTAAAGACGATTATGACCGTTTTTACACTTTATTCGTTAAGGAAGTTCGTGATGGTAAATTAGGTCAATACACCTT
AGATCAGGTGGGAGATATGG

Downstream 100 bases:

>100_bases
AAAGTTCTGTTTTTAGCAGAATTTTTTTCTTTTTTACGAATAAATGAAGTAAGGAGGTAACTTAGTGAATCATTTTGAAC
TTTATAAGCTAAAGAAAGCT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKVIQAELAEEERLEAMLSYEKALYKKGYKAIAGIDEVG
RGPLAGPVVAACVILPKYCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYDFAKNAGYGTKEHLQGLKAY
GITPIHRKSFEPVKSMCCDSTNP

Sequences:

>Translated_263_residues
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKVIQAELAEEERLEAMLSYEKALYKKGYKAIAGIDEVG
RGPLAGPVVAACVILPKYCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYDFAKNAGYGTKEHLQGLKAY
GITPIHRKSFEPVKSMCCDSTNP
>Mature_262_residues
PTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKVIQAELAEEERLEAMLSYEKALYKKGYKAIAGIDEVGR
GPLAGPVVAACVILPKYCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEG
QLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYDFAKNAGYGTKEHLQGLKAYG
ITPIHRKSFEPVKSMCCDSTNP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=183, Percent_Identity=46.9945355191257, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_STRP1 (Q99ZM6)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269312.1
- RefSeq:   YP_282246.1
- ProteinModelPortal:   Q99ZM6
- SMR:   Q99ZM6
- EnsemblBacteria:   EBSTRT00000000114
- EnsemblBacteria:   EBSTRT00000029063
- GeneID:   3572014
- GeneID:   901277
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_1162
- KEGG:   spz:M5005_Spy_0883
- GeneTree:   EBGT00050000028203
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   SPYO160490:SPY1162-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0883-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28763; Mature: 28632

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKVIQAELAEEERLEAMLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKALYKKGYKAIAGIDEVGRGPLAGPVVAACVILPKYCKIKGLNDSKKIPKAKHETIYQ
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHH
AVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEGQLTQPDYLLIDAMTLDIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
SQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYDFAKNAGYGTKEHLQGLKAY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
GITPIHRKSFEPVKSMCCDSTNP
CCCCHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKVIQAELAEEERLEAMLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKALYKKGYKAIAGIDEVGRGPLAGPVVAACVILPKYCKIKGLNDSKKIPKAKHETIYQ
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHH
AVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEGQLTQPDYLLIDAMTLDIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
SQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYDFAKNAGYGTKEHLQGLKAY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
GITPIHRKSFEPVKSMCCDSTNP
CCCCHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296