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The map label for this gene is dacA [H]

Identifier: 15675077

GI number: 15675077

Start: 894068

End: 895303

Strand: Direct

Name: dacA [H]

Synonym: SPy_1093

Alternate gene names: 15675077

Gene position: 894068-895303 (Clockwise)

Preceding gene: 15675076

Following gene: 15675079

Centisome position: 48.26

GC content: 40.29

Gene sequence:

>1236_bases
ATGATAAAAAGGTGGGTTATCCTTTTTTTTATGGTATTAACTTGTTCAGGGTTTGGTAAAACAGTTCTCGCAGCTGATTT
TCAAGTGGGAGCAGAACATGCGATTGTCGTAGAGGCAGACTCTGGGAGAGTTCTGTATGAGAAAGACGCTAAGACGCCAG
ATGCTATTGCCTCTTTGACCAAATTGGTGACGGCTTATCTGGTTTTAGATAAGGTTAAATCAGGCCAGCTGCAATTATCA
GATCAAGTTAATCTCTCAGATTATGCCTTCGAGTTAACAAAAGATAGGTCTTTAAGTAATGTACCTTTTGACAAAAAAAC
TTATTCAGTTCAAGACCTGTTGACAGCTACTTTGGTAGCAAGCTCAAATAGCGCTGCTATTGCGTTAGCCGAAAAAGTGG
CGGGCTCAGAGCTTCATTTTGTAAATCAAATGAGAGAACAGCTATCTCATTGGGGGATTACTTCTGGTAAAATCCTCAAC
GCTTCAGGACTGCCGAATGAGGTGCTAAAGGACCATCGTTATCCTGGTTCTGCGTTGGAAGAAGAGAATATGTTGAGTGC
TCAGGATGTTGCCATTGTGACGATGCATTTACTGGAAGATTTTCCTGAGATTTTAGAGATCACTAAACAAACAGAAGTTG
ATTTTGCTGGTAATTCTATTAAAAGTTTTAATCAACTCTTACCAGGTATGGCTAAGGGTAGAGCAGGAGTGGATGGGCTA
AAGACAGGAACGACAGATTTGGCAGGTCATTGCCTTGTGGTAACTTCTATTGAAAATGGTATGAGGCTGATTACCGTTAT
TCTAAATGCTGATGGATCTGATAAAAACCAAAACACGCGGTTTGAGCAAGCAAATCGCTTGTTGGACTATGTGGCTAGAA
CTTATTGTCGTCGTAAGATTCTTAAAAAAGGAAGTCTTGTCTCGGAGCGATCGCTCCCTATTCAAGATGGCCAAGTAAAA
GAACTACCGATATCTGTTGCCGAAGATGTAACCATCATTTTGCAACAGGGTGAACAAGTTCCCAAACCTAAACAATTTAT
GATATCAGAGACTTCTTTATTAGCACCGATTACTAAAGGTGAAGTGGTTGCTTATTTGACGTCTCCAAGAATAACAGATC
AGTCAGTGAGGTATTTAAAGGAACCTAAGCGTATTCCTCTTAAAGCTAGCCAATCTCTAAAAAAAGCCTCTGACTTACAG
CTGTGGTGGCGAGATTTTCTTGAAAAAAGACGTTAA

Upstream 100 bases:

>100_bases
TTGCTTTATGAGCTTGTTTAAGCTACTAAAGGTGTGTGCCACTATACTTCCTTCCAAATTTCTGAGAATTTTTGTTATAA
TAGTAACTATGAAAGATATA

Downstream 100 bases:

>100_bases
AGATAAGAATAAAATAATTTTAAAACAATCAAAAAAAGACAGTTATGAAAGAATTTTTTCATAACTGTCTTTTTAGTTTA
CAGTGAGTATGATTTGGAAT

Product: putative D,D-carboxypeptidase, penicillin-binding protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLS
DQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
ASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGNSIKSFNQLLPGMAKGRAGVDGL
KTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVK
ELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQ
LWWRDFLEKRR

Sequences:

>Translated_411_residues
MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLS
DQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
ASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGNSIKSFNQLLPGMAKGRAGVDGL
KTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVK
ELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQ
LWWRDFLEKRR
>Mature_411_residues
MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLS
DQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
ASGLPNEVLKDHRYPGSALEEENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGNSIKSFNQLLPGMAKGRAGVDGL
KTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVK
ELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQ
LWWRDFLEKRR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=365, Percent_Identity=30.958904109589, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786851, Length=349, Percent_Identity=27.7936962750716, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082022, Length=381, Percent_Identity=26.509186351706, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45497; Mature: 45497

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDAKTPDAIASLT
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHH
KLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVA
HHHHHHHHHHHCCCCCEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
SSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPGSALE
CCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCCCCCCC
EENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGNSIKSFNQLLPGMAKGRAGVDGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCCCCCCCCC
KTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKI
CCCCHHHCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKKGSLVSERSLPIQDGQVKELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKG
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHEEECHHHCCCCCCC
EVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR
CEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRVLYEKDAKTPDAIASLT
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHH
KLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVPFDKKTYSVQDLLTATLVA
HHHHHHHHHHHCCCCCEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
SSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILNASGLPNEVLKDHRYPGSALE
CCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHCCCCCCCCC
EENMLSAQDVAIVTMHLLEDFPEILEITKQTEVDFAGNSIKSFNQLLPGMAKGRAGVDGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHCCCCCCCCC
KTGTTDLAGHCLVVTSIENGMRLITVILNADGSDKNQNTRFEQANRLLDYVARTYCRRKI
CCCCHHHCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LKKGSLVSERSLPIQDGQVKELPISVAEDVTIILQQGEQVPKPKQFMISETSLLAPITKG
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHEEECHHHCCCCCCC
EVVAYLTSPRITDQSVRYLKEPKRIPLKASQSLKKASDLQLWWRDFLEKRR
CEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]