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The map label for this gene is rnz

Identifier: 15674944

GI number: 15674944

Start: 767076

End: 768005

Strand: Direct

Name: rnz

Synonym: SPy_0924

Alternate gene names: 15674944

Gene position: 767076-768005 (Clockwise)

Preceding gene: 15674943

Following gene: 15674945

Centisome position: 41.41

GC content: 40.54

Gene sequence:

>930_bases
ATGGAATTACAATTTTTAGGAACTGGAGCTGGTCAACCAGCCAAGCAGCGCAATGTGTCAAGTCTTGCCTTGAAATTACT
AGACGAAATCAATGAAGTCTGGATGTTTGATTGCGGTGAAGGAACGCAAAGACAAATTTTAGAAACGACGATTAAGCCAC
GAAAAATAAGAAAGATTTTCATTACTCATTTGCATGGAGACCACATTTTTGGATTACCAGGATTTTTATCAAGTCGGTCT
TTTCAAGCTAGTGAAGAACAGACAGATCTTGATATTTATGGACCAATTGGCATTAAAACTTACGTGTTAACTAGTCTAAA
AGTTTCTGGAGCGCGTGTACCTTATCAGATTCATTTTCATGAATTTGATGACAAATCATTAGGTAAAATAATGGAAACAG
ATAAGTTTGAGGTCTATGCAGAGCGTCTGGCGCATACCATTTTTTGCATGGGTTATCGAGTAGTTCAAAAAGACTTAGAA
GGAACCCTAGACGCAGAAGCCTTAAAAGCAGCGGGTGTTCCTTTTGGACCACTCTTTGGCAAAATTAAAAATGGTCAAGA
CGTTGAGTTGGAAGACGGCCGCCTGATTTGTGCCAAAGATTATATTTCTGCCCCTAAAAAAGGCAAAATCATCACCATTA
TCGGCGATACGCGCAAAACGTCTGCCAGTGTTAAACTCGCTAAAGATGCAGATGTTTTGGTTCATGAATCCACCTATGGT
AAGGGTGACGAGCGGATTGCGAGAAATCACGGGCATTCTACCAACATGCAGGCAGCACAAATCGCTCATGAAGCAGGCGC
AAAACGCCTCTTGCTCAATCATGTATCAGCTCGCTTTTTAGGGCGTGATTGTCGCCAGATGGAAAAAGATGCCGCCACTA
TATTTGAAAATGTTAAAATGGTACAAGATTTAGAGGAAGTGATTATTTAA

Upstream 100 bases:

>100_bases
AGTTATGGGCTCTTAGCCAAACATTTTCTGGTCAAAAGTATTATGATTTTGCCCAACGAGAATTTATGATTTACATCACC
CAATAGAAAGAATAGATTGA

Downstream 100 bases:

>100_bases
TGGCACAAAGAATCATTGTTATCACGGGAGCTTCTGGAGGACTGGCTCAGGCAATTGTTAAGCAGTTACCCAAGGAAGAC
AGCTTGATTTTATTAGGACG

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQRQILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRS
FQASEEQTDLDIYGPIGIKTYVLTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTRKTSASVKLAKDADVLVHESTYG
KGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVSARFLGRDCRQMEKDAATIFENVKMVQDLEEVII

Sequences:

>Translated_309_residues
MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQRQILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRS
FQASEEQTDLDIYGPIGIKTYVLTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTRKTSASVKLAKDADVLVHESTYG
KGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVSARFLGRDCRQMEKDAATIFENVKMVQDLEEVII
>Mature_309_residues
MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQRQILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRS
FQASEEQTDLDIYGPIGIKTYVLTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTRKTSASVKLAKDADVLVHESTYG
KGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVSARFLGRDCRQMEKDAATIFENVKMVQDLEEVII

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=351, Percent_Identity=35.3276353276353, Blast_Score=204, Evalue=6e-53,
Organism=Escherichia coli, GI87082078, Length=305, Percent_Identity=44.9180327868852, Blast_Score=266, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_STRP1 (P60199)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269118.1
- RefSeq:   YP_282088.1
- ProteinModelPortal:   P60199
- SMR:   P60199
- EnsemblBacteria:   EBSTRT00000000479
- EnsemblBacteria:   EBSTRT00000028163
- GeneID:   3572168
- GeneID:   901077
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0924
- KEGG:   spz:M5005_Spy_0725
- GeneTree:   EBGT00050000027751
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- BioCyc:   SPYO160490:SPY0924-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0725-MONOMER
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 34430; Mature: 34430

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQRQILETTIKPRKIRKIF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCHHHHHEEE
ITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYVLTSLKVSGARVPYQIHFH
EEEECCCEEECCCCHHCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCEEEEEE
EFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLEGTLDAEALKAAGVPFGPLFG
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
KIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTRKTSASVKLAKDADVLVHESTYG
CCCCCCCCEECCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCC
KGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVSARFLGRDCRQMEKDAATIFENVKM
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQDLEEVII
HHHHHHHHC
>Mature Secondary Structure
MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQRQILETTIKPRKIRKIF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHCCHHHHHEEE
ITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYVLTSLKVSGARVPYQIHFH
EEEECCCEEECCCCHHCCCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCEEEEEE
EFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLEGTLDAEALKAAGVPFGPLFG
ECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
KIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTRKTSASVKLAKDADVLVHESTYG
CCCCCCCCEECCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCC
KGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVSARFLGRDCRQMEKDAATIFENVKM
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQDLEEVII
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296