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The map label for this gene is pyrF

Identifier: 15674922

GI number: 15674922

Start: 744642

End: 745334

Strand: Direct

Name: pyrF

Synonym: SPy_0900

Alternate gene names: 15674922

Gene position: 744642-745334 (Clockwise)

Preceding gene: 15674921

Following gene: 15674923

Centisome position: 40.2

GC content: 41.7

Gene sequence:

>693_bases
ATGAAAGAAGAACGCCCAATTATTGCTTTAGACTTTTCATCATTTGAGGAGACTAAAGCATTTTTAGACTTATTTCCAGC
AGAAGAAAAACTATACGTGAAAATTGGTATGGAATTATATTATGCGCAAGGACCAGATATTGTTCGGTATATCAAATCGT
TAGGGCATAATGTATTTTTGGATTTAAAATTACATGATATTCCAAATACTGTTCGTGCTGCTATGGCAGTTCTTAAAGAA
TTAGATATTGATATGGCAACTGTTCATGCAGCTGGTGGTGTAGAGATGCTAAAAGCAGCTCGAGAAGGACTAGGTCAAGG
CCCTACCTTAATTGCGGTAACGCAGCTAACTTCAACCAGTGAAGACCAAATGCGTGGTGATCAAAATATTCAAACGAGTC
TTTTAGAGTCTGTTTTGCATTACAGCAAGGGGGCAGCTAAAGCTCAGCTAGATGGGGCGGTTTGTTCGGCACAAGAAGTA
GAAGCTATCAAGGCGGTGACGCCTACGGGATTTACTTGCTTAACTCCTGGCATTCGTCCTAAAGGCAGTAATATTGGCGA
TCAAAAACGAGTGATGACTCCAAACCAAGCAAGACGCATTGGAAGTGATTATATTGTGGTAGGGCGACCAATTACCCAGG
CAAAAGACCCAGTGGCTGCTTACCAAGCCATAAAAGCTGAGTGGGCAGGCTAA

Upstream 100 bases:

>100_bases
AAAAGGGAATTCCCTTTTAGTTTGCTGTAGCCTCGCTTCTTTGGTAGCGAGGCTTTTTGGTGCTTAATGAGATATTGACA
CATTATAGAAAGGATAAGTT

Downstream 100 bases:

>100_bases
CAGATAAGATTAGGAAACCAACACTTGTCTGTTAAAGAAGAAAAAGGAGAATAAGAAATGACACTAGCAAGCCAAATTGC
AACTCAATTACTTGATATTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQGPDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKE
LDIDMATVHAAGGVEMLKAAREGLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEV
EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPVAAYQAIKAEWAG

Sequences:

>Translated_230_residues
MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQGPDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKE
LDIDMATVHAAGGVEMLKAAREGLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEV
EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPVAAYQAIKAEWAG
>Mature_230_residues
MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQGPDIVRYIKSLGHNVFLDLKLHDIPNTVRAAMAVLKE
LDIDMATVHAAGGVEMLKAAREGLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEV
EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPVAAYQAIKAEWAG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=43.2314410480349, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_STRP1 (Q9A077)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_269096.1
- RefSeq:   YP_282066.1
- ProteinModelPortal:   Q9A077
- SMR:   Q9A077
- EnsemblBacteria:   EBSTRT00000000654
- EnsemblBacteria:   EBSTRT00000028712
- GeneID:   3572215
- GeneID:   901055
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0900
- KEGG:   spz:M5005_Spy_0703
- GeneTree:   EBGT00050000027603
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   SPYO160490:SPY0900-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0703-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24976; Mature: 24976

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 63-63 BINDING 11-11 BINDING 34-34 BINDING 117-117 BINDING 179-179 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQGPDIVRYIKSLGHNVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCCEEE
DLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAAREGLGQGPTLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
EDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEVEAIKAVTPTGFTCLTPGIRP
HHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHCCHHHHHHHHHCCCCCCEEECCCCCC
KGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPVAAYQAIKAEWAG
CCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQGPDIVRYIKSLGHNVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHHHCCCCEEE
DLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAAREGLGQGPTLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
EDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGAVCSAQEVEAIKAVTPTGFTCLTPGIRP
HHHHCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHCCHHHHHHHHHCCCCCCEEECCCCCC
KGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPVAAYQAIKAEWAG
CCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296