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The map label for this gene is dnaG [H]

Identifier: 15674826

GI number: 15674826

Start: 640206

End: 642020

Strand: Direct

Name: dnaG [H]

Synonym: SPy_0781

Alternate gene names: 15674826

Gene position: 640206-642020 (Clockwise)

Preceding gene: 15674824

Following gene: 15674827

Centisome position: 34.56

GC content: 38.57

Gene sequence:

>1815_bases
ATGGGATTTTTATGGGGAGGTGACGATTTGGCAATTGACAAAGAAATGATTTCCCAAGTAAAAAATAGCGTTAATATTGT
CGATGTCATTGGAGAAGTGGTCAAACTTTCCCGATCAGGGCGGCATTACCTCGGGCTTTGCCCATTTCATAAGGAAAAGA
CACCCTCTTTTAATGTTGTTGAAGACAGACAATTTTTTCACTGCTTTGGCTGTGGAAAATCAGGGGATGTTTTTAAATTT
ATTGAGGAATACCGCCAAGTCCCCTTCTTAGAAAGTGTTCAGATTATTGCCGATAAGACTGGTATGTCGCTTAATATACC
GCCAAGTCAGGCAGTACTTGCTAGCCAACACAAGCACCCTAATCACGCTTTGATGACACTTCATGAGGATGCTGCTAAAT
TTTACCATGCAGTTTTGATGACCACTACCATTGGTCAAGAAGCTAGGAAGTACCTTTACCAGAGAGGCTTGGATGACCAA
TTAATTGAGCATTTCAATATTGGTTTAGCCCCAGATGAGTCAGATTATCTTTATCAAGCTCTTTCTAAAAAATACGAGGA
AGGTCAATTGGTTGCTTCAGGATTGTTTCACTTGTCCGATCAATCCAATACCATTTACGACGCCTTTCGAAATCGTATCA
TGTTTCCCTTATCAGATGACCGAGGGCATATTATTGCCTTTTCAGGACGTATCTGGACGGCAGCTGATATGGAAAAGAGA
CAGGCAAAGTATAAAAATTCAAGAGGAACAGTTCTTTTTAACAAATCTTATGAATTGTATCATCTGGACAAGGCAAGGCC
TGTTATTGCCAAAACCCATGAAGTGTTTCTAATGGAAGGGTTTATGGACGTGATTGCCGCTTACCGTTCCGGCTATGAAA
ATGCTGTTGCTTCAATGGGGACGGCATTGACTCAAGAACATGTCAATCACCTTAAGCAAGTCACTAAAAAAGTTGTTTTG
ATTTATGATGGTGACGATGCTGGACAACATGCTATTGCAAAATCACTAGAATTGCTTAAAGATTTTGTTGTCGAAATTGT
CAGAATCCCCAATAAAATGGATCCTGACGAATTTGTACAACGGCATTCCCCAGAAGCATTTGCAGATTTGCTTAAGCAGT
CACGGATCAGTAGTGTTGAATTTTTTATTGATTACCTAAAACCTACTAATGTAGACAATTTGCAATCACAAATTGTTTAT
GTGGAGAAAATGGCACCATTGATTGCTCAATCACCATCCATCACAGCTCAACATTCGTATATTAACAAGATTGCTGATTT
GTTGCCAAACTTTGACTATTTTCAAGTAGAACAATCAGTAAATGCATTAAGGATTCAAGATAGGCAAAAACATCAAGGTC
AAATAGCTCAAGCCGTCAGCAATCTTGTGACCTTACCAATGCCAAAAAGTTTGACAGCTATTGCTAAGACAGAAAGTCAT
CTCATGCATCGGCTCTTACATCATGACTATTTATTAAATGAATTTCGACATCGTGATGATTTTTATTTTGATACCTCTAC
CTTAGAATTACTTTATCAACGGCTGAAGCAACAAGGACACATTACATCTTATGATTTGTCAGAGATGTCAGAGGAAGTTA
ACCGTGCTTATTACAATGTTTTAGAAGAAAACCTTCCCAAAGAAGTAGCTCTTGGTGAGATTGATGATATTTTATCCAAA
CGTGCCAAACTTTTAGCAGAGCGCGATCTTCACAAACAAGGGAAAAAAGTTAGAGAATCTAGTAACAAAGGCGATCATCA
AGCGGCTCTAGAAGTACTAGAACATTTTATTGCGCAGAAACGAAAAATGGAATAG

Upstream 100 bases:

>100_bases
TTAAGCATAGCATAATTAGTAAACAAAATCCAATGGAATCACTTCAGTTTTTAAATTTACAAAAAAGAGTTTCGGTGGTA
TAATAAAACGTAAAACTAAA

Downstream 100 bases:

>100_bases
AGGAAATTATGACAAAACAAAAAGAAATAACAACTTTTAACGTTCAAGTTGCGGAGTTTATTCGCCATCACAAAAAAGAA
GGAACAGCGATTGATGATGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKF
IEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADMEKR
QAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVL
IYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVY
VEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKHQGQIAQAVSNLVTLPMPKSLTAIAKTESH
LMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSK
RAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME

Sequences:

>Translated_604_residues
MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKF
IEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADMEKR
QAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVL
IYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVY
VEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKHQGQIAQAVSNLVTLPMPKSLTAIAKTESH
LMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSK
RAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME
>Mature_603_residues
GFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFI
EEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQL
IEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADMEKRQ
AKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLI
YDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYV
EKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKHQGQIAQAVSNLVTLPMPKSLTAIAKTESHL
MHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKR
AKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=434, Percent_Identity=32.4884792626728, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69141; Mature: 69009

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVV
CCCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
EDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHP
CCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCHHHHHHCCCCC
NHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQA
CHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
LSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADMEKR
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCEECEECCCCCCEEEECCCEEEHHHHHHH
QAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMG
HHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQ
HHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSY
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
INKIADLLPNFDYFQVEQSVNALRIQDRQKHQGQIAQAVSNLVTLPMPKSLTAIAKTESH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
LMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNV
HHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKME
HCCC
>Mature Secondary Structure 
GFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFNVV
CCEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
EDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHP
CCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCHHHHHHCCCCC
NHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQA
CHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHH
LSKKYEEGQLVASGLFHLSDQSNTIYDAFRNRIMFPLSDDRGHIIAFSGRIWTAADMEKR
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCEECEECCCCCCEEEECCCEEEHHHHHHH
QAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVASMG
HHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQ
HHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSY
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
INKIADLLPNFDYFQVEQSVNALRIQDRQKHQGQIAQAVSNLVTLPMPKSLTAIAKTESH
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHH
LMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYYNV
HHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKME
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]