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The map label for this gene is glnH [H]

Identifier: 15674823

GI number: 15674823

Start: 638456

End: 639268

Strand: Direct

Name: glnH [H]

Synonym: SPy_0778

Alternate gene names: 15674823

Gene position: 638456-639268 (Clockwise)

Preceding gene: 15674822

Following gene: 15674824

Centisome position: 34.47

GC content: 35.79

Gene sequence:

>813_bases
ATGAATTTTAAAAAAATAAGATTTGGTTTTTTGCTTTGCCTCCTACTTTATCCTTTAGCAGCTTGTAACAAGTCTGAACA
ACTTAATCACTACGAAAGAATTAAAAAAACAAGAAAATTAGTCGTTGCTGTAAGCCCTGATTATGCTCCTTTTGAATTTA
AAGCTTTAGTTAATGGAAAAGATACTATTGTTGGAGCAGATGTTCAATTAGCTCAGGCTATTGCTGATGAATTAGACGTT
GATCTTGAGCTTTCACCAATGAGTTTTGATAATGTTTTGTCTAGTTTACAGACTGGTAAAGCTGATCTTGCTATTTCAGG
AATCTCACATACTAAAGAAAGAGCTAAAGTATATGATTTTTCAATTCCTTACTATCAGGCAGAAAACGCTATAGTGATGA
GAGCATCTGATGCTAAAGTGACCAAAAATATTAGTGACCTAAACGGTAAGAAAGTAGCCGCTCAAAAAGGTAGTATCGAG
GAAGGTTTAGTTAAAATACAATTACCAAAGGCCAATCTGATTTCTTTGACTGCTATGGGAGAAGCCATTAATGAACTAAA
AGCAGGACAAGTCTATGCAGTTACCTTAGAAGCACCTGTAGCTGCTGGTTTTTTAGCCCAACATAAGGATCTGGCTTTAG
CACCCTTTAGCTTAAAAACCAGTGATGGAGATGCCAAAGCAGTGGCCCTTCCTAAAAATAGTGGAGACTTAACCAAAGCC
GTTAATAAGGTTATTGCTAAGTTAGATGAGCAAGAACGGTACAAGTCATTTATTGCAGAGACAATAGCACTAACGAAAAA
TACTATGAAGTAA

Upstream 100 bases:

>100_bases
TATAGTTGAGGATTTTACGAAATGCTTGAAAAGCTATTGACCTTTGAATAATAATACAATATAATAAGTTTTATTAGCGA
CAAAAAGGAATAAATATACT

Downstream 100 bases:

>100_bases
TAAGGTTAATAAAAACTTTATTAGTATCCGTTGGAAAAGTGTTTGGAATAAACAATAAAAGAGGTTTGACTTTTTTAAAA
AAATAATGTAATATAATTGG

Product: putative ABC transporter substrate-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDV
DLELSPMSFDNVLSSLQTGKADLAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAVALPKNSGDLTKA
VNKVIAKLDEQERYKSFIAETIALTKNTMK

Sequences:

>Translated_270_residues
MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDV
DLELSPMSFDNVLSSLQTGKADLAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAVALPKNSGDLTKA
VNKVIAKLDEQERYKSFIAETIALTKNTMK
>Mature_270_residues
MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDV
DLELSPMSFDNVLSSLQTGKADLAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAVALPKNSGDLTKA
VNKVIAKLDEQERYKSFIAETIALTKNTMK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=219, Percent_Identity=34.703196347032, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787088, Length=216, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788649, Length=221, Percent_Identity=28.9592760180996, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788648, Length=218, Percent_Identity=26.1467889908257, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29336; Mature: 29336

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFKALVNGK
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEECCC
DTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISGISHTKERAKVYDF
CEEEECCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHCEEEEE
SIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIEEGLVKIQLPKANLISLTAMG
CCCEEECCCEEEEEECCCHHHCCHHHCCCCEEECCCCCCCCCEEEEEECCCCEEEHHHHH
EAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAVALPKNSGDLTKA
HHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEECCEEEECCCCCCEEEEECCCCCHHHHH
VNKVIAKLDEQERYKSFIAETIALTKNTMK
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFKALVNGK
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEECCC
DTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISGISHTKERAKVYDF
CEEEECCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHCEEEEE
SIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIEEGLVKIQLPKANLISLTAMG
CCCEEECCCEEEEEECCCHHHCCHHHCCCCEEECCCCCCCCCEEEEEECCCCEEEHHHHH
EAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAVALPKNSGDLTKA
HHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEECCEEEECCCCCCEEEEECCCCCHHHHH
VNKVIAKLDEQERYKSFIAETIALTKNTMK
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]