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The map label for this gene is ligA

Identifier: 15674800

GI number: 15674800

Start: 611246

End: 613204

Strand: Direct

Name: ligA

Synonym: SPy_0751

Alternate gene names: 15674800

Gene position: 611246-613204 (Clockwise)

Preceding gene: 15674799

Following gene: 15674801

Centisome position: 33.0

GC content: 40.53

Gene sequence:

>1959_bases
ATGAAAAAACGCATAAAAGAATTAACAGATCTTCTTAACCGTTACCGTTATGACTATTATACTAAAGATGCCCCATCAGT
TTCTGACAGTGACTATGATAAGCTTTATCGTGAACTGGTGACTTTAGAGCAGTCATATCCCGAGTATGTCTTGCAAGATA
GCCCAACGCAGCAGGTTGGAGGGACTATTTTAAAGGGGTTTGAAAAATATCGACATCAGTATCCGCTTTTTAGTTTGCAG
GATGCTTTTTCACGTGAGGAGCTGGATGCTTTTGATAAGCGCGTCAAGGCCGAATTTCCGAATGCCACCTATCTAGCAGA
GTTAAAAATTGATGGTTTATCTATTTCCTTGAGTTATGAGAATGGCTTCTTACAGGTTGGAGCAACACGGGGTGATGGCA
ATATCGGTGAAAATATCACAGAAAATATCAAAAAAATAAAGGATATTCCTTACCAATTGAGTGAGCCTTTAACCATCACC
GTTCGAGGAGAGGCTTACATGTCTCGTCAGTCCTTTAAAGCGATCAATGAAGCACGCCAAGAAAATGGGGAGACTGAATT
TGCTAATCCTAGAAATGCTGCCGCTGGGACACTAAGGCAGCTAGATACGTCAGTGGTTGCTAAGCGTCAACTAGCCACTT
TTTTGTATCAAGAAGCTAGTCCGACAGCTAGAAATCAACAAAATGAGGTCTTAGCAGAATTAGCAGATCTAGGTTTTTCA
GTAAATCCGTATTATCAACTCACATCGTCTATGGATGAGATTTGGGATTTTATCAAAACTATTGAGGCAAAAAGAGATCA
ATTAGCTTATGACATAGATGGCGTTGTTATTAAAGTTAATAGCCTAGCCATGCAAGAAGAGCTAGGGTTTACGGTTAAAG
CTCCACGCTGGGCCATTGCTTACAAATTTCCAGCAGAAGAAAAAGAGGCAGAAATCCTTTCGGTTGACTGGACAGTGGGA
CGAACAGGCGTGGTCACACCTACAGCGAATCTAACTCCTGTGCAACTAGCGGGGACAACCGTGAGTCGTGCAACCCTACA
TAATGTGGATTATATTGCCGAAAAAGATATCCGTATTGGCGATACAGTGATTGTATATAAGGCAGGGGACATTATTCCTG
CTGTCTTAAATGTCGTTATGAGTAAGCGGAACCAGCAAGAAGTCATGCTTATTCCTAAACTATGCCCATCTTGTGGTAGT
GAATTAGTTCATTTTGAAGATGAGGTTGCTTTGCGTTGTATTAATCCGTTGTGTCCTAGTTTGATTCAGCGAAGTTTGGA
GCATTTTGCAAGTCGTGATGCCATGAACATTACTGGGCTGGGACCAGCTATTGTGGAAAAACTATTTTTAGCGGGGTTTG
TTCATGATGTTGCTGATATTTACCAGTTGACCAAAGAAGATTTCATGCAATTAGATGGCATCAAAGAAAAGTCAGCTGAT
AAATTGCTCGCTGCGATTGAGGCTTCTAAATCAAATTCAGCTGAAAAACTGTTATTTGGTCTAGGAATTCGTCATATTGG
CTCTAAGGTTAGTCGCTTAATTTTAGAGGTATACGGAGATATTTCTGCTCTTTTAACCGCTAAAGAAGAGGAAATCGCAA
GGATAGATGGTCTTGGCTCAACGATTGCTCAATCACTTACTCAATACTTTGAACAAAAAACGGCTGCGATTTTAGTAGAC
GAGTTAAAGACAGCAGGTGTCAACATGCATTACTCAGGTCAAAAAGTTAATAGTGACGCAGCGCTTTTTGGTCTAACTGT
TGTTTTAACTGGCAAATTAAATCAGCTGAACCGAAATGAGGCCAAAGATAAATTAGAGGCATTAGGTGCCAAAGTTACTG
GAAGTGTATCTAAGAAAACAGATTTAGTTATTGCAGGTAGTGACGCTGGTTCTAAGCTAGAAAAAGCAAAGAGCTTAGGA
ATTCGGATTGAAGATGAAGATTGGCTACGCCAGCTTTAA

Upstream 100 bases:

>100_bases
CTTTAGCTAATAGTACCATATGGAGTGGCCAACGATTACTTTTGATGTTGTTATGATATAGAAATAGCTCATTTGTTATC
TTTTATGATAAAATAAAAGT

Downstream 100 bases:

>100_bases
GTGAGGTAATGTTATGAAAAAACAATTGAGAGCACGTCTCATTTATAATCCAACGTCAGGACAAGAACTTATGAGAAAAA
GTGTTCCTGAAGTTTTAGAC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Sequences:

>Translated_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL
>Mature_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=42.7503736920777, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082305, Length=558, Percent_Identity=22.7598566308244, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_STRP1 (Q9A0J5)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268974.1
- RefSeq:   YP_281936.1
- HSSP:   O87703
- ProteinModelPortal:   Q9A0J5
- EnsemblBacteria:   EBSTRT00000001486
- EnsemblBacteria:   EBSTRT00000027688
- GeneID:   3572319
- GeneID:   900931
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0751
- KEGG:   spz:M5005_Spy_0573
- GeneTree:   EBGT00050000028051
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   SPYO160490:SPY0751-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0573-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 72421; Mature: 72421

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 109-109 BINDING 107-107 BINDING 130-130 BINDING 164-164 BINDING 278-278 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTITVRGEAYMSRQSFKAINEARQ
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC
>Mature Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPYQLSEPLTITVRGEAYMSRQSFKAINEARQ
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTSVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKEDFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296