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The map label for this gene is gyrB

Identifier: 15674782

GI number: 15674782

Start: 581680

End: 583632

Strand: Direct

Name: gyrB

Synonym: SPy_0727

Alternate gene names: 15674782

Gene position: 581680-583632 (Clockwise)

Preceding gene: 15674781

Following gene: 15674783

Centisome position: 31.4

GC content: 39.94

Gene sequence:

>1953_bases
ATGATTGAAGAAAATAAACATTTTGAAAAAAAAATGCAAGAATACGATGCCAGTCAAATTCAGGTTCTAGAAGGGCTGGA
GGCTGTCCGCATGCGTCCAGGGATGTATATTGGCTCGACAGCTAAAGAGGGTTTGCATCATTTAGTCTGGGAAATTGTTG
ACAACTCAATTGACGAAGCATTAGCAGGTTTTGCCTCTCATATTAAAGTCTTTATTGAAGCAGATAATTCCATTACAGTA
GTAGATGATGGCCGTGGAATTCCAGTTGATATCCAAGCCAAGACAGGACGTCCCGCCGTTGAAACAGTTTTTACAGTCTT
ACACGCAGGTGGTAAATTTGGTGGAGGCGGCTATAAGGTTTCTGGAGGATTACATGGTGTAGGGTCATCTGTTGTTAATG
CTTTATCAACACAATTAGATGTACGTGTTTATAAAAACGGCCAAATTCATTACCAAGAATTTAAACGCGGGGCTGTTGTA
GCAGATCTTGAGGTCATTGGAACCACTGATGTGACTGGCACGACCGTACACTTTACACCCGATCCAGAAATTTTTACCGA
AACGACTCAGTTTGATTACAGTGTTTTAGCAAAACGTATTCAAGAGTTAGCCTTTTTGAATCGTGGTTTAAAAATTTCCA
TTACAGATAAGCGCTCAGGTATGGAACAAGAAGAACATTTCCTTTATGAAGGTGGAATTGGTTCTTATGTTGAATTTTTA
AATGATAAAAAAGATGTTATCTTTGAAACGCCCATCTATACAGATGGTGAATTAGAAGGTATTGCAGTTGAAGTAGCCAT
GCAATACACGACTAGCTATCAAGAAACAGTCATGAGTTTTGCTAATAATATTCATACTCATGAAGGTGGAACGCATGAAC
AAGGCTTTAGAGCGGCTCTTACTCGGGTCATCAATGACTACGCTAAGAAAAATAAAATTCTTAAAGAAAATGAGGACAAT
TTGACAGGAGAAGATGTTCGTGAAGGTTTGACGGCGGTAATTTCTGTTAAGCATCCAAATCCTCAATTTGAAGGTCAAAC
CAAAACAAAATTGGGCAACTCAGAAGTGGTTAAGATCACTAATCGTCTCTTTAGTGAGGCCTTTCAACGTTTTCTTTTGG
AAAACCCACAAGTTGCTCGTAAGATTGTGGAAAAAGGGATTTTGGCTTCTAAAGCTAGAATTGCAGCTAAGCGAGCCCGC
GAAGTCACCCGCAAAAAATCAGGCTTAGAAATTTCAAACTTACCTGGAAAATTAGCAGACTGTTCGTCAAATGACGCTAA
CCAAAACGAACTTTTCATCGTCGAAGGAGATTCAGCGGGTGGGTCGGCCAAATCAGGTCGTAACCGAGAGTTTCAAGCTA
TCTTGCCTATTCGCGGTAAAATTTTGAACGTGGAAAAAGCAACTATGGATAAGATTCTTGCCAACGAAGAAATTAGAAGT
CTCTTTACCGCTATGGGTACAGGTTTTGGTGCAGATTTTGACGTGTCAAAAGCTCGCTACCAAAAGCTGGTTATCATGAC
CGATGCCGATGTGGATGGCGCTCATATTAGAACCTTACTTTTAACCTTGATTTACCGCTTTATGAGACCTGTTCTAGAAG
CTGGCTATGTTTACATCGCCCAGCCACCTATTTATGGTGTTAAGGTCGGTAGTGAGATTAAAGAGTATATTCAGCCAGGT
ATTGATCAAGAAGACCAATTAAAAACAGCTCTTGAAAAATATAGTATTGGTCGTTCAAAACCAACTGTTCAACGTTATAA
AGGTCTTGGGGAAATGGATGACCATCAACTTTGGGAAACTACTATGGATCCTGAAAATCGTTTGATGGCGCGTGTGACAG
TTGATGATGCCGCAGAAGCAGATAAAGTATTTGATATGTTAATGGGAGATCGTGTTGAACCAAGACGTGATTTCATTGAG
GAAAATGCGGTTTATAGTACACTGGATATTTAG

Upstream 100 bases:

>100_bases
AATTGGAGATCGGCTAATTGATAAACAAGCAGGACAAGCAGCGGGTTTTAATACCTTGCTTGTTGACGGTAGAAAAAACC
TATTGGAGATAGTAACGTAG

Downstream 100 bases:

>100_bases
TCACCTTTTTAGTTTCACAAGGAATCTGCTACCAAAGAGGACGAATTGACTAAAAATCATGATGCATGATGCCAACAGCT
TTGTTAGTTAGTACGTCTTC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFLYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI

Sequences:

>Translated_650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFLYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI
>Mature_650_residues
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITV
VDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFLYEGGIGSYVEFL
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDN
LTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAR
EVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPG
IDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIE
ENAVYSTLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=684, Percent_Identity=26.6081871345029, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19913406, Length=676, Percent_Identity=26.3313609467456, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI48994957, Length=564, Percent_Identity=53.7234042553192, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=40.5572755417957, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17535065, Length=667, Percent_Identity=24.4377811094453, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=23.731884057971, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=232, Percent_Identity=25.4310344827586, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=592, Percent_Identity=25.3378378378378, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136538, Length=648, Percent_Identity=25.1543209876543, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_STRP1 (Q9A0L0)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268956.1
- RefSeq:   YP_281916.1
- ProteinModelPortal:   Q9A0L0
- EnsemblBacteria:   EBSTRT00000001268
- EnsemblBacteria:   EBSTRT00000028586
- GeneID:   3572376
- GeneID:   900912
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0727
- KEGG:   spz:M5005_Spy_0553
- GeneTree:   EBGT00050000028252
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK05644
- BioCyc:   SPYO160490:SPY0727-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0553-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 72272; Mature: 72272

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEA
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
LAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHHEEEEEECCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVVADLEVIGTTDVTGTTVHFTP
CCCCCHHHHHHHHHHHHHEEEEEEECCCEEHHHHHCCCEEEEEEEEECCCCCCCEEEECC
DPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFLYEGGIGSYVEFL
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHEEEECCHHHHHHHH
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAAL
CCCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TRVINDYAKKNKILKENEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKIT
HHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHH
NRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLAD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
CSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHH
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIA
HHHHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
QPPIYGVKVGSEIKEYIQPGIDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWET
CCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHC
TMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIEENAVYSTLDI
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEGLHHLVWEIVDNSIDEA
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHH
LAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHHEEEEEECCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVVADLEVIGTTDVTGTTVHFTP
CCCCCHHHHHHHHHHHHHEEEEEEECCCEEHHHHHCCCEEEEEEEEECCCCCCCEEEECC
DPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKRSGMEQEEHFLYEGGIGSYVEFL
CCCCEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHEEEECCHHHHHHHH
NDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQETVMSFANNIHTHEGGTHEQGFRAAL
CCCCCEEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TRVINDYAKKNKILKENEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKIT
HHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHH
NRLFSEAFQRFLLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLAD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCC
CSSNDANQNELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIRS
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHHH
LFTAMGTGFGADFDVSKARYQKLVIMTDADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIA
HHHHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEE
QPPIYGVKVGSEIKEYIQPGIDQEDQLKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWET
CCCCEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHC
TMDPENRLMARVTVDDAAEADKVFDMLMGDRVEPRRDFIEENAVYSTLDI
CCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296