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The map label for this gene is regR [H]

Identifier: 15674700

GI number: 15674700

Start: 502974

End: 503969

Strand: Reverse

Name: regR [H]

Synonym: SPy_0627

Alternate gene names: 15674700

Gene position: 503969-502974 (Counterclockwise)

Preceding gene: 15674701

Following gene: 15674697

Centisome position: 27.21

GC content: 35.24

Gene sequence:

>996_bases
ATGCAGCGCAAAGTAACCATCAAAGATATTGCTGAATTAGCGAAAACTTCAAAAACAACAGTTTCTTTCTACTTAAATGG
CAGGTTTGACAAGATGTCTGAAGAAACTAAAAACCGCATCAGCGAGAGTATCAAAGCTACTAATTATAAACCGAGCATTG
CTGCTAGAAGTCTCAATGCAAAATCGACTAAACTCATTGGTGTTGTTATAGGTGACATCACAAACAGTTTTTCAAACCAA
ATTGTCAAAGGAATTGAAAGCAAAGCCCAGGAATTTGGTTACCAAATTATCATCGGAAACAGCAACTACGACCCTTCTCG
CGAAGATGAACTCATTGAAAAGATGCTCAATCTTGGTGTTGATGGTTTTATTATTCAACCAACTTCTAACTTTAGAAAAT
ATTCACGTATTATCGATATTAAAAAAAAGAAGGTTGTCTTTTTTGATAGCCAACTTTACGAACATCGCACAAACTGGGTC
AAAACTAATAATTATGATGCTGTTTATGATACCATTCAACAATGTATCGATAAAGGTTATGAACATTTTATCATGATCAC
GGGTAACCCCAATTTACTTAGCACTCGTATTGAACGCGCTTCAGGCTTCATTGATGTGTTAGAAGCTAATCATCTTACTC
ATCAAGAAATGATTATTGATGAAAATCAGACTTCTTCAGAGGCAATTGCTCAGTTTTTGCAAGGATCACTGACTAAAAAG
AGTTTGGTTTTTGTCCCCAATTGTTGGGCTCTTCCAAAAGTATTTACAGCTATGAAGTCTCTTAAATTTAATATACCAGA
GATTGGACTAGTAGGATTTGATAACATTGAGTGGACAAAATTTTCTTCACCAACACTAACAACTATTATCCAACCCGCCT
ATGAAGAAGGCGAACAGGCTACTAAGATTTTAATCGATGATATTGAAGGTCACAGTCAAGAAGCCAAACAACAAATTTTT
GACTGTCAAGTCAATTGGCAAGAATCAACTTTTTAA

Upstream 100 bases:

>100_bases
CCATATCACGAATGAGATTATTCGATTGAAACACTAAATCTTTTACGAGTCCTTATGTTATAATATGACTAACCATTACT
ATTTTAAAGGAGTGTATGCA

Downstream 100 bases:

>100_bases
TGTCAGGCATACCAATAGTCCTTATCATTGTAACGATTATAAAAAAAGGTTTGAGACAATTGTCTCAAACCTTTTAGCGT
CTATTAAATTGCTTTTTCAT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Kdg operon repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESIKATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF

Sequences:

>Translated_331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESIKATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF
>Mature_331_residues
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESIKATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQ
IVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIF
DCQVNWQESTF

Specific function: Transcriptional repressor of the kdg operon for pectin utilization [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=26.2048192771084, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=30.5466237942122, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787948, Length=328, Percent_Identity=26.8292682926829, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=24.7648902821317, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=29.4478527607362, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=22.7118644067797, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=21.9435736677116, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788474, Length=323, Percent_Identity=22.6006191950464, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786268, Length=204, Percent_Identity=29.9019607843137, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787906, Length=297, Percent_Identity=22.8956228956229, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=24.5283018867925, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786540, Length=327, Percent_Identity=22.9357798165138, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790689, Length=330, Percent_Identity=24.5454545454545, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37672; Mature: 37672

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESIKATNYKPSIAARSLNA
CCCEEEHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGV
CCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCC
DGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWVKTNNYDAVYDTIQQCIDKGY
CCEEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
EEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHEECCCCCCHHHHHHHHHCCCCCC
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHHHCCHHHHCCHHH
TKILIDDIEGHSQEAKQQIFDCQVNWQESTF
HHHEEECCCCCHHHHHHHHHCCEECCCCCCC
>Mature Secondary Structure
MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRISESIKATNYKPSIAARSLNA
CCCEEEHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSNYDPSREDELIEKMLNLGV
CCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCC
DGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWVKTNNYDAVYDTIQQCIDKGY
CCEEECCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCC
EHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMIIDENQTSSEAIAQFLQGSLTKK
EEEEEEECCCCHHHHHHHHHCCHHHHHHCCCCCHHHHEECCCCCCHHHHHHHHHCCCCCC
SLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNIEWTKFSSPTLTTIIQPAYEEGEQA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHHHCCHHHHCCHHH
TKILIDDIEGHSQEAKQQIFDCQVNWQESTF
HHHEEECCCCCHHHHHHHHHCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]