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The map label for this gene is murN [H]

Identifier: 15674693

GI number: 15674693

Start: 494496

End: 495731

Strand: Reverse

Name: murN [H]

Synonym: SPy_0615

Alternate gene names: 15674693

Gene position: 495731-494496 (Counterclockwise)

Preceding gene: 15674694

Following gene: 15674688

Centisome position: 26.76

GC content: 36.41

Gene sequence:

>1236_bases
ATGGCACTAATTGAAATTTCACAAGAACAATTTGACCATTACTGCCACAGTCTCGTCCATCATTCCTTTATCCAAACCTC
AGAAATGGCCAGCTTGATGGCTAAAAGAGGGGCAAAACCACAATTTCTTGGACTTGAAAAAGATGGTGAACTAAAGGTTG
CTGCTATGGTCTTTAGCCAAAAAGTGGCTGGAGGTTGGCGTATGGAACTCAATGCAGGCCCTAATACCAATCATCCCGAA
GAATTAGAACATTTTTACACTCAACTTAAAGACTATGCCAAACAAAAAGACGTTATCGAACTCATTTTAAAACCTTACGA
CAATTATCAGTCATTTGATACTGATGGCATCCCTATTTCTCGTCCAAACACTGACCTTATTAGCCTTTTAACGGCGTTAG
GATATAAACATGACGGTTTAAAAACAGGCTATCCTGAGGGTGAACCTGTTTGGCATTACGTTAAAAAGTTAGAAGGAATT
GATTCCTCACGTTTAACCCGTTCCTTCAGCAAAAAAGGAAAAGCTTTAATAAAAAAAGCTAATACTTTTGGTATTAAATT
ACGTCAACTGAAACGAGATGAGTTACACCATTTCAAAGAAATTACTGAAGCAACCTCTGATCGTAGAGATTACCTTGACA
AATCATTATCTTACTATCAGGACTTTTATGATAGTTTTGGAGACTCTTGTGAATTTATGGTTGCGACTCTTAATTTTGAA
GATTACCTTAATAACCTTAAACAAAGACAACTACAACTAGCAACCAGCATCAACAAAGTTAAAGGAGACCTTGGCAAAAA
TCCTCATTCTGAAAAAAAGCAAAATAGATTAAAAGAACTATCCAGCCAGTTCGAAACCTTTCAGGTAAGAATTTCTGAAG
CTCTCCATTTTCTTGAAGAGTATGGAACTAAAGATGTGTTCCTTGCGGGAAGTCTTTTTATCTATACCGAACAAGAGGCC
GTCTACCTCTTTTCAGGATCTTACCCCAAATTTAATAAATTTTATAGCCCAGCTCTATTACAAGAGCATGCTATGCTCAA
GGCAATTCACAAGGGCATCAAACAATATAATTTTCTTGGTATTACTGGAAAGTTTGATGGTTCAGACGGTGTCTTGCGCT
TTAAACAGAATTTCAATGGCTTTATTTTGCAAAAACCAGGGACATTTAGATGTTATCCATTTCCAATCAAATATCATTTC
ATTAGATTGGCAAAAAAGCTATTAAACCGCTATTAG

Upstream 100 bases:

>100_bases
ATCGAAGAATTTGCAGGTGAATTCAACATTCCTGTCGGCCTTGTTTCAAGCTTAGCCATCTTAACCTATAACTTAAGAAA
AAAATTAAGGAGCACAACTA

Downstream 100 bases:

>100_bases
GCTAATGCATTTGCCTGTAGAATCACCTTTATTCTCTTATAGTAAGAGTTAAGTCATCTAAAAAAGAAAAGGATTTAACC
TTTTCTTTTTTAGATGACTT

Product: putative peptidoglycan branched peptide synthesis protein

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance B [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPE
ELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISRPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
DSSRLTRSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEA
VYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHF
IRLAKKLLNRY

Sequences:

>Translated_411_residues
MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPE
ELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISRPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
DSSRLTRSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEA
VYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHF
IRLAKKLLNRY
>Mature_410_residues
ALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEE
LEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISRPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGID
SSRLTRSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFED
YLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAV
YLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFI
RLAKKLLNRY

Specific function: Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 4 and 5 of the pentaglycine bridge, using glycyl- tRNA(Gly) as donor. Involved in resistance to methicillin [H]

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 47529; Mature: 47398

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQ
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHH
KVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPIS
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGIDSSRLTRSFSKKGKALIKKA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
NTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHH
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLG
HCCCCEEEECEEEEEECCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
ITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFIRLAKKLLNRY
EEEEECCCCCEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQ
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHH
KVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPIS
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGIDSSRLTRSFSKKGKALIKKA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
NTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHH
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLG
HCCCCEEEECEEEEEECCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
ITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFIRLAKKLLNRY
EEEEECCCCCEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA