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The map label for this gene is rnc

Identifier: 15674631

GI number: 15674631

Start: 429696

End: 430388

Strand: Direct

Name: rnc

Synonym: SPy_0531

Alternate gene names: 15674631

Gene position: 429696-430388 (Clockwise)

Preceding gene: 15674630

Following gene: 15674632

Centisome position: 23.2

GC content: 37.23

Gene sequence:

>693_bases
ATGAAACAGCTTGAAGAGTTACTCTCAACATCTTTTGATATTCAATTTAATGATTTGACTTTACTAGAAACAGCTTTTAC
CCACACCTCTTACGCTAACGAGCATCGTCTCCTAAACGTTTCACATAATGAACGCTTGGAATTTTTAGGAGACGCTGTTC
TACAATTGATTATTTCAGAGTATTTATTTGCAAAGTATCCTAAAAAGACAGAAGGAGATATGTCTAAGTTACGTTCCATG
ATTGTACGTGAGGAAAGCTTGGCAGGTTTTTCACGATTTTGTTCTTTTGACGCTTATATCAAACTAGGAAAAGGTGAGGA
AAAGTCAGGTGGTCGCCGTCGTGATACTATTTTAGGAGACTTATTTGAAGCCTTTTTAGGTGCTCTTTTATTAGATAAGG
GGATTGATGCAGTACGTCGCTTCTTGAAACAAGTCATGATTCCTCAGGTGGAAAAAGGGAATTTTGAAAGGGTTAAAGAC
TATAAAACTTGTCTTCAAGAGTTTTTGCAAACAAAAGGTGATGTGGCTATTGATTATCAAGTCATTAGTGAGAAAGGACC
TGCCCATGCTAAACAATTTGAGGTATCGATAGTTGTTAATGGTGCTGTCCTTAGTAAAGGTTTAGGTAAGTCTAAAAAAT
TAGCGGAGCAAGATGCTGCTAAAAATGCCCTTGCCCAACTTAGTGAGGTTTAG

Upstream 100 bases:

>100_bases
ATGTTTAATAAATCACGAAAAATCCACTTAGAGTCGGTTTTCTAGGTGGATTTTTGGTATACTAAATAAGGTCTACTGAC
AAATATGAAAGGGATTTTTT

Downstream 100 bases:

>100_bases
ATGTTTTTAAAAGAAATTGAGTTGGAAGGGTTCAAGTCCTTTGCGGACAAGACTAAAATTGAATTTGATAAGGGAGTAAC
TGCAGTCGTAGGACCAAATG

Product: ribonuclease III

Products: NA

Alternate protein names: Ribonuclease III; RNase III

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSM
IVREESLAGFSRFCSFDAYIKLGKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQDAAKNALAQLSEV

Sequences:

>Translated_230_residues
MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSM
IVREESLAGFSRFCSFDAYIKLGKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQDAAKNALAQLSEV
>Mature_230_residues
MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSM
IVREESLAGFSRFCSFDAYIKLGKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQDAAKNALAQLSEV

Specific function: Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs

COG id: COG0571

COG function: function code K; dsRNA-specific ribonuclease

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase III domain

Homologues:

Organism=Homo sapiens, GI155030234, Length=233, Percent_Identity=30.4721030042918, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI155030236, Length=233, Percent_Identity=30.4721030042918, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1788920, Length=204, Percent_Identity=37.7450980392157, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI193202651, Length=237, Percent_Identity=33.7552742616034, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17506883, Length=237, Percent_Identity=33.7552742616034, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323895, Length=230, Percent_Identity=26.9565217391304, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137682, Length=232, Percent_Identity=34.051724137931, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNC_STRP1 (P66670)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268805.1
- RefSeq:   YP_281801.1
- ProteinModelPortal:   P66670
- SMR:   P66670
- EnsemblBacteria:   EBSTRT00000000022
- EnsemblBacteria:   EBSTRT00000028583
- GeneID:   3572493
- GeneID:   900755
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0531
- KEGG:   spz:M5005_Spy_0438
- GeneTree:   EBGT00050000028295
- HOGENOM:   HBG744556
- OMA:   ALTHKSF
- ProtClustDB:   PRK00102
- BioCyc:   SPYO160490:SPY0531-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0438-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00104
- InterPro:   IPR001159
- InterPro:   IPR014720
- InterPro:   IPR000999
- InterPro:   IPR011907
- Gene3D:   G3DSA:3.30.160.20
- Gene3D:   G3DSA:1.10.1520.10
- SMART:   SM00358
- SMART:   SM00535
- TIGRFAMs:   TIGR02191

Pfam domain/function: PF00035 dsrm; PF00636 Ribonuclease_3; SSF69065 RNase_III

EC number: =3.1.26.3

Molecular weight: Translated: 25848; Mature: 25848

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50137 DS_RBD; PS00517 RNASE_3_1; PS50142 RNASE_3_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISE
CHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
YLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKLGKGEEKSGGRRRDTILGD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCCCHHHHHHHH
LFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKDYKTCLQEFLQTKGDVAIDYQ
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
VISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQDAAKNALAQLSEV
EECCCCCCCHHHEEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNERLEFLGDAVLQLIISE
CHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
YLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKLGKGEEKSGGRRRDTILGD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCCCHHHHHHHH
LFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKDYKTCLQEFLQTKGDVAIDYQ
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
VISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQDAAKNALAQLSEV
EECCCCCCCHHHEEEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296