Click here to switch to the map view.

The map label for this gene is pfs [H]

Identifier: 15674570

GI number: 15674570

Start: 369396

End: 370091

Strand: Direct

Name: pfs [H]

Synonym: SPy_0447

Alternate gene names: 15674570

Gene position: 369396-370091 (Clockwise)

Preceding gene: 15674569

Following gene: 15674571

Centisome position: 19.94

GC content: 42.67

Gene sequence:

>696_bases
ATGAAAATAGGAATTATTGCTGCTATGGAGGAGGAGTTGTCCCTGCTCTTAGCAAATCTTTTGGATGCTCAAGAACATCA
GGTGCTTTCAAAAACTTACTATACGGGTCGTTTTGGCAAGCATGAGCTCATTTTGGTACAGTCGGGTGTTGGAAAAGTGA
TGTCTGCTATGACAGTTGCGATCTTGGTTGAGCATTTCAAAGCCCAAGCTATTATCAATACAGGATCAGCAGGGGCAGTC
GCTTCACATTTAGCGATCGGAGATGTGGTGGTTGCAGATCGGTTAGTGTATCATGATGTGGATGCTACTGCATTTGGTTA
TGCTTATGGTCAAATGGCTGGTCAACCATTATACTATGATTGTGATCCACAGTTTGTGGCTATCTTCAAACAAGTCCTAA
AACACGAAAAGACAAATGGCCAAGTTGGTTTGATTGCAACAGGAGACAGTTTTGTTGCTGGTCAGGACAAGATTGACCAG
ATCAAAACAGCTTTTAGCGACGTGTTAGCAGTAGAAATGGAAGGCGCAGCTATTGCTCAAGCAGCACATACTGCAGGGAA
ACCCTTCATCGTTGTTCGTGCGATGAGTGATACCGCAGCGCATGATGCCAATATTACTTTTGATCAATTTATTATAGAAG
CTGGCAAGCGTTCGGCCCAGACATTAATGACATTTTTAGAAAACTTACCTGTTTAA

Upstream 100 bases:

>100_bases
TTGCAATCTAAATTAAATGTGATTTTGATAGCTGTTATGCTGTTAATTGCCATCTTGGTATATGCTATCTTTTATCTTTA
AAAAAACGTAAGGAAATGAT

Downstream 100 bases:

>100_bases
CTCACTTTATCGTGAGACAATTAGCTGGATAATCTCTATTTTAGGAGTCTGGTCACTTCTTGTTTTGAAACCTAAAAATA
AGAATTGCAATCACTTGACC

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELILVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAV
ASHLAIGDVVVADRLVYHDVDATAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRSAQTLMTFLENLPV

Sequences:

>Translated_231_residues
MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELILVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAV
ASHLAIGDVVVADRLVYHDVDATAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRSAQTLMTFLENLPV
>Mature_231_residues
MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELILVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAV
ASHLAIGDVVVADRLVYHDVDATAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRSAQTLMTFLENLPV

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=229, Percent_Identity=41.4847161572052, Blast_Score=174, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 24761; Mature: 24761

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELILVQSGVGKVMSAMTVA
CCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHH
ILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDATAFGYAYGQMAGQPLYYD
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEE
CDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQIKTAFSDVLAVEMEGAAIAQ
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHH
AAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRSAQTLMTFLENLPV
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELILVQSGVGKVMSAMTVA
CCEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHH
ILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDATAFGYAYGQMAGQPLYYD
HHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEE
CDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQIKTAFSDVLAVEMEGAAIAQ
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHH
AAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRSAQTLMTFLENLPV
HHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]