Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 15674551

GI number: 15674551

Start: 350781

End: 352781

Strand: Direct

Name: metG [H]

Synonym: SPy_0422

Alternate gene names: 15674551

Gene position: 350781-352781 (Clockwise)

Preceding gene: 15674550

Following gene: 15674552

Centisome position: 18.94

GC content: 44.08

Gene sequence:

>2001_bases
ATGATGAAAAAACCATTTTACATTACGACACCCATTTATTACCCATCTGGGAAATTACACATTGGTTCTGCCTATACAAC
TATTGCTTGTGATGTCTTGGCACGCTACAAGCGCCTGATGGGCCATGAGGTCTTTTACCTCACAGGACTAGATGAACACG
GACAAAAGATTCAAACCAAGGCAAAAGAAGCTGGTATTACACCTCAAACCTACGTTGATAACATGGCAAAGGATGTCAAA
GCCCTTTGGCAATTGCTAGATATTTCCTATGACACATTTATCCGGACAACGGACGATTACCACGAAGAGGTCGTTGCTGC
TGTCTTTGAAAAATTGCTGGCGCAAGATGACATTTACCTTGGCGAATACTCTGGTTGGTATTCTGTCTCTGATGAGGAAT
TTTTCACTGAAAGCCAATTAAAAGAAGTCTTTAGAGATGAAGACGGTCAGGTTATTGGAGGGATTGCCCCATCAGGTCAT
GAGGTCGAGTGGGTCTCTGAAGAATCCTACTTCCTACGCCTTAGCAAATACGATGATCGTCTTGTGGCCTTCTTTAAAGA
GCGACCTGACTTTATCCAACCAGATGGTCGTATGAATGAAATGGTGAAAAACTTTATCGAGCCAGGCCTAGAAGATTTGG
CTGTTAGCCGTACTACCTTTACATGGGGTGTACCTGTTCCATCAGACCCAAAACACGTGGTTTATGTCTGGATCGATGCC
CTCTTAAACTACGCGACAGCTCTTGGTTATCGTCAAGCTAATCACGCTAACTTTGATAAGTTTTGGAATGGAACGGTTTT
CCACATGGTTGGAAAAGATATCTTGCGTTTTCATAGCATCTACTGGCCAATTTTACTCATGATGCTTGATTTACCAATGC
CAGACCGCTTAATTGCCCATGGTTGGTTTGTCATGAAAGACGGTAAAATGTCTAAATCAAAAGGCAATGTGGTCTATCCA
GAAATGTTAGTTGAGCGCTTTGGACTTGATCCTCTTCGTTATTACCTCATGCGTTCTTTGCCAGTTGGTTCAGACGGGAC
CTTTACCCCTGAAGATTATGTGGGTCGTATCAACTATGAGTTAGCCAACGACCTTGGCAATTTATTGAACCGTACCGTTG
CCATGATCAACAAGTATTTTGACGGCACGGTGCCAGCTTATGTTGACAATGGAACTGCCTTTGATGCTGACTTAAGTCAA
CTGATTGATGCTCAGCTAGCAGACTACCATAAGCACATGGAAGCGGTGGACTACCCACGCGCCTTGGAGGCTGTTTGGAC
CATCATCGCTCGTACCAACAAATACATCGATGAAACCGCTCCTTGGGTGCTCGCTAAAGAAGATGGTGACAAAGCTCAAC
TAGCCAGTGTCATGGCACACTTAGCAGCCAGCTTGCGTCTGGTCGCTCATGTGATCCAACCCTTTATGATGGAAACATCA
GCTGCTATCATGGCCCAGCTTGGTTTGGAACCTGTTTCTGATTTGTCAACACTTGCTCTAGCAGATTTTCCTGCCAATAC
AAAGGTTGTTGCCAAAGGGACACCGATTTTCCCACGTCTTGACATGGAAGCTGAGATTGACTACATCAAAGCCCAAATGG
GTGACAGCTCAGCTATTTCCCAAGAAAAAGAATGGGTCCCTGAAGAGGTTGCGCTCAAATCTGAAAAAGACGTCATCACA
TTTGAAACCTTTGATGCTGTGGAAATCCGTGTGGCAGAAGTCAAGGAAGTGTCAAAAGTGGAAGGTTCAGAAAAATTGCT
GCGCTTTAGAGTAGATGCAGGAGACGGGCAAGACCGTCAAATCTTGTCAGGTATTGCCAAATTTTACCCCAACGAACAAG
AATTAGTGGGTAAAAAACTACAAATTGTTGCTAACCTGAAACCGCGTAAAATGATGAAAAAATACATCAGTCAAGGGATG
ATTCTATCAGCAGAGCATGGAGATCAGCTAACGGTCTTAACCGTTGATTCCTCTGTTCCAAATGGAAGTATCATTGGCTG
A

Upstream 100 bases:

>100_bases
TCCATTAGCCTTGAGGGTTGAATTTAAACAGGAAAAAACGAAATCCTACACTATGCATCTCAATCGTACTTTCATTTGAG
TATGGTTCTGAAAGGAAACT

Downstream 100 bases:

>100_bases
TGCTGACCTATTATTCAAAAAACCTAAGCAAGAACATGTGCTTAGGTTTTTTTGATTGCCTTCAAGAAAGACGAATAACG
AGATGGGTGATATGACATCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETS
AAIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGM
ILSAEHGDQLTVLTVDSSVPNGSIIG

Sequences:

>Translated_666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETS
AAIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGM
ILSAEHGDQLTVLTVDSSVPNGSIIG
>Mature_666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYDDRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETS
AAIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGM
ILSAEHGDQLTVLTVDSSVPNGSIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=507, Percent_Identity=34.7140039447732, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI14043022, Length=587, Percent_Identity=27.427597955707, Blast_Score=191, Evalue=3e-48,
Organism=Escherichia coli, GI1788432, Length=696, Percent_Identity=27.8735632183908, Blast_Score=269, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=31.25, Blast_Score=251, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17540794, Length=571, Percent_Identity=26.6199649737303, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=29.6875, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=479, Percent_Identity=32.776617954071, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=25.4901960784314, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=33.4669338677355, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655311, Length=566, Percent_Identity=25.0883392226148, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75263; Mature: 75263

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTK
CCCCCEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYDDR
ECCCCCEECCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEEEECCCHH
LVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHH
LLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAH
HHHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHH
LANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAIS
HHHHHHHCCCCHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHC
QEKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQ
HHHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVP
HHHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCC
NGSIIG
CCCCCC
>Mature Secondary Structure
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTK
CCCCCEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYDDR
ECCCCCEECCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCCEEEEEECCCHH
LVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHH
LLNYATALGYRQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAH
HHHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHH
LANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAVWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRLVAHVIQPFMMETS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIMAQLGLEPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAIS
HHHHHHHCCCCHHHHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHC
QEKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQ
HHHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEEEECCCCCCHHH
ILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKKYISQGMILSAEHGDQLTVLTVDSSVP
HHHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCC
NGSIIG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]