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The map label for this gene is tcyA [H]

Identifier: 15674482

GI number: 15674482

Start: 279342

End: 280184

Strand: Direct

Name: tcyA [H]

Synonym: SPy_0317

Alternate gene names: 15674482

Gene position: 279342-280184 (Clockwise)

Preceding gene: 15674481

Following gene: 15674483

Centisome position: 15.08

GC content: 38.67

Gene sequence:

>843_bases
ATGATTAAAAAACAGTATTTAGGAAGGGCAACAATTGCCCTAGCATCGACCCTTGTGCTAGCAGCTTGTGGGTCATCAAA
AACAGCTGAGTCAGGAAATCAGGGGAGTTCTAAAGAAGTCTTATTTGCAACGGTTGGAACAACAGCTCCCTTTTCTTATG
AAAAAGGTGGTCAACTAACTGGCTACGATATCGAGGTGGCTAAAGCAGTCTTTAAAGGATCGGATGATTACAAGGTGTCC
TTTAAAAAAACAGAATGGTCTTCTATCTTTACAGGTTTGGACTCAGGAAAATACCAAATGGGTGGTAACAACATTTCCTT
TACCAAAGAGCGTTCAGCTAAGTACCTTTTTTCTTATCCAATCGGATCAACACCTTCTGTTTTAGTTGTTCCAAAAGACA
GTGATATCAAGAGCTTTGATGATATCCAAGGGCATACGACACAAGTTGTTCAAGGGACTACAAGTGTTGCACAGTTAGAA
GACTTTAATAAGAAACACAGTGACAACCCGGTTACTCTAAAATTCACCAATGAGAACATTACTCAAATGTTAACCAATTT
GAGTGAAGGCAAGGCAGATTTCAAGATTTTTGACGCTCCAACGGTTAACGCCATTATTAAAAATCAAGGGTTAGATAATC
TCAAAACAATTGAACTCACCTCAACAGAGCAACCATTTATTTATTTTATCTTTAGTCAAGATCAAGAGAAATTGCAAAGC
TTTGTTAACAAGCGTATTAAAGAGTTGACTGCTGATGGAACTCTTAGCAAGCTTGCCAAAGAGCACCTTGGCGGTGATTA
CGTGCCATCAGATAAAGAGTTGAAACTTCCTACAGCTAACTAA

Upstream 100 bases:

>100_bases
TGAACAATAGTAAATCAGTATCAACTTGATAGGAATCAATTATTAGTGAACTCTAATTAAAAATGGTAAACTAGACACAA
GAACAAAAAGGAGAGATAGG

Downstream 100 bases:

>100_bases
ACCATCTATTAACCAGTTATTCATCTAGAAGACTGGTTTTTCTGATCGGAAACAATATTATAGTTTATGAGGTTATGTTA
TAAAAATTGCAAATAGCCTT

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFATVGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVS
FKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIELTSTEQPFIYFIFSQDQEKLQS
FVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKLPTAN

Sequences:

>Translated_280_residues
MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFATVGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVS
FKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIELTSTEQPFIYFIFSQDQEKLQS
FVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKLPTAN
>Mature_280_residues
MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFATVGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVS
FKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIELTSTEQPFIYFIFSQDQEKLQS
FVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKLPTAN

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=272, Percent_Identity=26.8382352941176, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787031, Length=257, Percent_Identity=26.8482490272374, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30623; Mature: 30623

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFATVGTTAPFSYEKGGQLT
CCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLFSYP
CCHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEECCCCEEEECCCCCEEEEEEC
IGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLEDFNKKHSDNPVTLKFTNENI
CCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHH
TQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIELTSTEQPFIYFIFSQDQEKLQS
HHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHH
FVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKLPTAN
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFATVGTTAPFSYEKGGQLT
CCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCC
GYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLFSYP
CCHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCEEECCCCEEEECCCCCEEEEEEC
IGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLEDFNKKHSDNPVTLKFTNENI
CCCCCEEEEEECCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHH
TQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIELTSTEQPFIYFIFSQDQEKLQS
HHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHH
FVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKLPTAN
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]