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The map label for this gene is nusG

Identifier: 15674369

GI number: 15674369

Start: 148676

End: 149215

Strand: Direct

Name: nusG

Synonym: SPy_0164

Alternate gene names: 15674369

Gene position: 148676-149215 (Clockwise)

Preceding gene: 15674368

Following gene: 15674370

Centisome position: 8.03

GC content: 41.11

Gene sequence:

>540_bases
ATGCTAGATTCATTTGATAAGGGCTGGTTCGTCCTCCAAACTTACTCAGGCTACGAAAACAAAGTAAAAGAAAACCTCTT
GCAACGTGCCCAAACCTATAACATGTTGGACAATATTTTGCGCGTCGAAATTCCGACACAAACGGTTAACGTTGAAAAAA
ATGGGCAAACCAAAGAAATCGAAGAAAATCGCTTTCCAGGGTATGTCTTGGTTGAAATGGTCATGACAGATGAAGCTTGG
TTTGTTGTGCGTAACACACCAAACGTTACAGGTTTTGTGGGATCACACGGGAACCGATCAAAACCAACACCACTTCTTGA
AGAAGAAATCCGTGCTATCTTGTTGTCTATGGGTCAAACCATTGATGTCTTTGATACCAATATCAAAGAAGGCGATGTGG
TTCAGATCATAGACGGTGCCTTTATGGGACAAGAAGGCCGTGTGGTAGAAATCGAAAACAACAAAGTGAAACTGATGCTC
AACATGTTTGGCTCTGAAACAGTAGCAGAAGTTGAACTTTATCAAATTGCAGAACTATAG

Upstream 100 bases:

>100_bases
CAGATACGTTGACAGCAGCCCTGCCTTTTATTATAATGAAGGTGATAGAAAACAGCGCAAAAGCCAAAAAAAGGGCTTTT
TTATAATGAAAGGAAACATC

Downstream 100 bases:

>100_bases
GCTCTCAAAGGTTTGATGGTATAAGTATTACCATCAGACCTTTTTTAGTTGGAAGTGAAGCGTGTGTACAAACAAACGGA
TGACTATTGTTAACATCATT

Product: transcription antitermination protein NusG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIPTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAW
FVVRNTPNVTGFVGSHGNRSKPTPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
NMFGSETVAEVELYQIAEL

Sequences:

>Translated_179_residues
MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIPTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAW
FVVRNTPNVTGFVGSHGNRSKPTPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
NMFGSETVAEVELYQIAEL
>Mature_179_residues
MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIPTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAW
FVVRNTPNVTGFVGSHGNRSKPTPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
NMFGSETVAEVELYQIAEL

Specific function: Influences transcription termination and antitermination. Acts as a component of the transcription complex, and interacts with the termination factor rho and RNA polymerase

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KOW domain

Homologues:

Organism=Escherichia coli, GI1790414, Length=180, Percent_Identity=35, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUSG_STRP1 (P68893)

Other databases:

- EMBL:   AE004092
- EMBL:   CP000017
- RefSeq:   NP_268543.1
- RefSeq:   YP_281502.1
- ProteinModelPortal:   P68893
- SMR:   P68893
- EnsemblBacteria:   EBSTRT00000000864
- EnsemblBacteria:   EBSTRT00000029091
- GeneID:   3572761
- GeneID:   900487
- GenomeReviews:   AE004092_GR
- GenomeReviews:   CP000017_GR
- KEGG:   spy:SPy_0164
- KEGG:   spz:M5005_Spy_0138
- GeneTree:   EBGT00050000027686
- HOGENOM:   HBG751162
- OMA:   VASGCEK
- ProtClustDB:   PRK05609
- BioCyc:   SPYO160490:SPY0164-MONOMER
- BioCyc:   SPYO293653:M5005_SPY0138-MONOMER
- InterPro:   IPR005824
- InterPro:   IPR001062
- InterPro:   IPR006645
- InterPro:   IPR014722
- InterPro:   IPR008991
- Gene3D:   G3DSA:2.30.30.30
- Gene3D:   G3DSA:3.30.70.940
- PRINTS:   PR00338
- SMART:   SM00739
- SMART:   SM00738
- TIGRFAMs:   TIGR00922

Pfam domain/function: PF00467 KOW; PF02357 NusG; SSF82679 Transcrpt_antiterm_NusG_N; SSF50104 Transl_SH3_like

EC number: NA

Molecular weight: Translated: 20386; Mature: 20386

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01014 NUSG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIPTQTVNVEKNGQTKEI
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCCH
EENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPTPLLEEEIRAILLSMGQT
HHCCCCCEEEEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
IDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLMLNMFGSETVAEVELYQIAEL
EEEEECCCCCCCEEEEECCEEECCCCCEEEEECCEEEEEEEECCCCCHHHHHEEEEECC
>Mature Secondary Structure
MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIPTQTVNVEKNGQTKEI
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEECCCCCCCCH
EENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPTPLLEEEIRAILLSMGQT
HHCCCCCEEEEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
IDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLMLNMFGSETVAEVELYQIAEL
EEEEECCCCCCCEEEEECCEEECCCCCEEEEECCEEEEEEEECCCCCHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296