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The map label for this gene is metG [H]

Identifier: 13474936

GI number: 13474936

Start: 4792812

End: 4794557

Strand: Direct

Name: metG [H]

Synonym: mlr5926

Alternate gene names: 13474936

Gene position: 4792812-4794557 (Clockwise)

Preceding gene: 13474935

Following gene: 13474937

Centisome position: 68.12

GC content: 61.57

Gene sequence:

>1746_bases
GTGCTGCACAACGATGGGCTCGAAAACCTAAACTTCGTCGATGTCTACTGGTGCGACGGCAAGGCGGCCCTCGAAGCTGC
TGCACAGGTCGCGATTCCCCGCGGGCCGATCTTCGTCTTCTCCACGCCGCCGACGCCTAATGGCGATCTGCATCTGGGGC
ATCTTTCTGGCCCTTATCTTGGCGCCGACGTCTACACGCGTTTCCTGCGCATGAAAGGGGTCGAAGCCTATCACCTGACT
GGAAGCGACGATTACCAGAGCTACGTCGCCACGCGTGCCGATGCCGAGCAATCGACGCCCGGAAAGGTGGCGCGCCACTA
CGCCGACGAGATTCGAGCCACGCTTGCGCTTCTCGACTGTGAGGTCCACAGCTTCCTCCCCACCTTGGGCGATAGCGCTT
ATGCAGAGTTTCAAGCCGCGTGTTTCCGCAGTCTTCTGAGCAGCACGGCGGTAGATCTGCGCCAGAGCCCTGCGCTTTTC
GATGCTGTGACAGGCGACTACCTTTACGAACCGGATGTAAGTGGCCTCTGCCCCGATTGCGGCGGCTCCGCTGGTGGCAA
CATCTGCGAGGAATGCGGCGCGCCGAACCTATGCCACGACCTAGATGCAGTCCGGTCGCGGCACTCGGCTGAGGCGCCGG
TGGTAGGCTTCATGCGCCGCCCCGAACTTGCGCTGGAACGGTGCTACGTCAATATCGACCGGCATTTAAGGGCTTCGGGC
GCCCCCGTTCGGATCATGGACCTCTTCGCGCGTCTACGCCAACGTGGCGACTTTTCCGTGCCCATCATCCACCCGTCGGA
CTGGGGGCTACCGGCCGAGGGGTTCCCGGGGCAGGTGACCTGGGTCTGGCCAGAGATGGCGTTTGGCTTTCTTTACAACA
TCCAGACGCTAGCTACATCGCTCGGTCGACACTGGAATGCGGCCCTGCCCAGCAAGGACTGGCAAATTGTCCATTTCTTC
GGCTTCGATAATTCCTTCTACCACGCTCTGCTTTATCCGGCGCTTTATGCTGAGGTCTTCTCCCACTGGACGCCGCGCAT
CCGGTATCATGTGAACGAGTTCTACCTGCTCGACGGTCAGAAGTTCTCGACAAGCCGCAGCCATGCGGTCTGGGGCAAAG
AGGTTCTGGGCCAAAAGACAGTCGATGTCGTCCGTCTTCATTTGGGCCTGACACGCCCGGAAGGTGAGCGGACGAACTTC
ACGCTGGACGCGCTACGCCGCACGGAGAACGAGGTGTTCCAAGGAATCTGGCTGAATTGGCTTGATGCGCTGCACGGGGA
AATCAAACAGGACTTTGGCGGCTGTGTGCCCGACGCAGGCGACTGGGCTCCCGCCGATCGCGCTTTCTTTGGTCGGATAG
AGATCCATCGCGCGGCGATGGAACGCGCCTATTCGGACGATGGCTTCTCCGTCCGCCGCGCGGCCGCGCAGGCCTGCGAT
TTCGTCAGCGATTGCGTAGTCTACCGACGGGCGCAAGACTATGAGGCGGCGCGACGTCTCTATCCCGCCCGCACCCGCAC
GTCGCTCGCCCTTCAGACGACGGCCGCTGCAATCCTGGCACAGACACTCGCGCCGCTGATGCCCCGATTTGCCAGCACGC
TGGCGCGAGGTATCGATGGCGTCTCGACCGAAACATGGCCCACGTCCGTCCGGCGCCTTGCGCCAGGAACAACCTTTGAT
CTCGGACCCGTGCTGGGCTTCTACGCCAGAGCAGACATTCCTGCTCTCAATGAAGAGGAGGCATAA

Upstream 100 bases:

>100_bases
CTTCGTGGTCCTGTCTGGAGCGGGCAAGGTCAGGACCGACCTTGGGGAAATCTCGGTCGCAGCCGGCGATGTCGTTCTGT
TCCATCCTTTTGAAGCGCAT

Downstream 100 bases:

>100_bases
TAGCCCATCGTAGTTTCGACCAGATTTGGGCCGCAATCAAAGCCGATGATGCCGAAGCTTTACTCGCGGCCACTGGCTGC
CGAAGGCCACTCCCAGACCA

Product: tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MLHNDGLENLNFVDVYWCDGKAALEAAAQVAIPRGPIFVFSTPPTPNGDLHLGHLSGPYLGADVYTRFLRMKGVEAYHLT
GSDDYQSYVATRADAEQSTPGKVARHYADEIRATLALLDCEVHSFLPTLGDSAYAEFQAACFRSLLSSTAVDLRQSPALF
DAVTGDYLYEPDVSGLCPDCGGSAGGNICEECGAPNLCHDLDAVRSRHSAEAPVVGFMRRPELALERCYVNIDRHLRASG
APVRIMDLFARLRQRGDFSVPIIHPSDWGLPAEGFPGQVTWVWPEMAFGFLYNIQTLATSLGRHWNAALPSKDWQIVHFF
GFDNSFYHALLYPALYAEVFSHWTPRIRYHVNEFYLLDGQKFSTSRSHAVWGKEVLGQKTVDVVRLHLGLTRPEGERTNF
TLDALRRTENEVFQGIWLNWLDALHGEIKQDFGGCVPDAGDWAPADRAFFGRIEIHRAAMERAYSDDGFSVRRAAAQACD
FVSDCVVYRRAQDYEAARRLYPARTRTSLALQTTAAAILAQTLAPLMPRFASTLARGIDGVSTETWPTSVRRLAPGTTFD
LGPVLGFYARADIPALNEEEA

Sequences:

>Translated_581_residues
MLHNDGLENLNFVDVYWCDGKAALEAAAQVAIPRGPIFVFSTPPTPNGDLHLGHLSGPYLGADVYTRFLRMKGVEAYHLT
GSDDYQSYVATRADAEQSTPGKVARHYADEIRATLALLDCEVHSFLPTLGDSAYAEFQAACFRSLLSSTAVDLRQSPALF
DAVTGDYLYEPDVSGLCPDCGGSAGGNICEECGAPNLCHDLDAVRSRHSAEAPVVGFMRRPELALERCYVNIDRHLRASG
APVRIMDLFARLRQRGDFSVPIIHPSDWGLPAEGFPGQVTWVWPEMAFGFLYNIQTLATSLGRHWNAALPSKDWQIVHFF
GFDNSFYHALLYPALYAEVFSHWTPRIRYHVNEFYLLDGQKFSTSRSHAVWGKEVLGQKTVDVVRLHLGLTRPEGERTNF
TLDALRRTENEVFQGIWLNWLDALHGEIKQDFGGCVPDAGDWAPADRAFFGRIEIHRAAMERAYSDDGFSVRRAAAQACD
FVSDCVVYRRAQDYEAARRLYPARTRTSLALQTTAAAILAQTLAPLMPRFASTLARGIDGVSTETWPTSVRRLAPGTTFD
LGPVLGFYARADIPALNEEEA
>Mature_581_residues
MLHNDGLENLNFVDVYWCDGKAALEAAAQVAIPRGPIFVFSTPPTPNGDLHLGHLSGPYLGADVYTRFLRMKGVEAYHLT
GSDDYQSYVATRADAEQSTPGKVARHYADEIRATLALLDCEVHSFLPTLGDSAYAEFQAACFRSLLSSTAVDLRQSPALF
DAVTGDYLYEPDVSGLCPDCGGSAGGNICEECGAPNLCHDLDAVRSRHSAEAPVVGFMRRPELALERCYVNIDRHLRASG
APVRIMDLFARLRQRGDFSVPIIHPSDWGLPAEGFPGQVTWVWPEMAFGFLYNIQTLATSLGRHWNAALPSKDWQIVHFF
GFDNSFYHALLYPALYAEVFSHWTPRIRYHVNEFYLLDGQKFSTSRSHAVWGKEVLGQKTVDVVRLHLGLTRPEGERTNF
TLDALRRTENEVFQGIWLNWLDALHGEIKQDFGGCVPDAGDWAPADRAFFGRIEIHRAAMERAYSDDGFSVRRAAAQACD
FVSDCVVYRRAQDYEAARRLYPARTRTSLALQTTAAAILAQTLAPLMPRFASTLARGIDGVSTETWPTSVRRLAPGTTFD
LGPVLGFYARADIPALNEEEA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=24.4230769230769, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788432, Length=365, Percent_Identity=22.7397260273973, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17540794, Length=520, Percent_Identity=23.2692307692308, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 64467; Mature: 64467

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNDGLENLNFVDVYWCDGKAALEAAAQVAIPRGPIFVFSTPPTPNGDLHLGHLSGPYL
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCC
GADVYTRFLRMKGVEAYHLTGSDDYQSYVATRADAEQSTPGKVARHYADEIRATLALLDC
CHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVHSFLPTLGDSAYAEFQAACFRSLLSSTAVDLRQSPALFDAVTGDYLYEPDVSGLCPDC
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCC
GGSAGGNICEECGAPNLCHDLDAVRSRHSAEAPVVGFMRRPELALERCYVNIDRHLRASG
CCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCC
APVRIMDLFARLRQRGDFSVPIIHPSDWGLPAEGFPGQVTWVWPEMAFGFLYNIQTLATS
CCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
LGRHWNAALPSKDWQIVHFFGFDNSFYHALLYPALYAEVFSHWTPRIRYHVNEFYLLDGQ
HCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECCC
KFSTSRSHAVWGKEVLGQKTVDVVRLHLGLTRPEGERTNFTLDALRRTENEVFQGIWLNW
CCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDALHGEIKQDFGGCVPDAGDWAPADRAFFGRIEIHRAAMERAYSDDGFSVRRAAAQACD
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FVSDCVVYRRAQDYEAARRLYPARTRTSLALQTTAAAILAQTLAPLMPRFASTLARGIDG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSTETWPTSVRRLAPGTTFDLGPVLGFYARADIPALNEEEA
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLHNDGLENLNFVDVYWCDGKAALEAAAQVAIPRGPIFVFSTPPTPNGDLHLGHLSGPYL
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCC
GADVYTRFLRMKGVEAYHLTGSDDYQSYVATRADAEQSTPGKVARHYADEIRATLALLDC
CHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVHSFLPTLGDSAYAEFQAACFRSLLSSTAVDLRQSPALFDAVTGDYLYEPDVSGLCPDC
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCC
GGSAGGNICEECGAPNLCHDLDAVRSRHSAEAPVVGFMRRPELALERCYVNIDRHLRASG
CCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCC
APVRIMDLFARLRQRGDFSVPIIHPSDWGLPAEGFPGQVTWVWPEMAFGFLYNIQTLATS
CCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
LGRHWNAALPSKDWQIVHFFGFDNSFYHALLYPALYAEVFSHWTPRIRYHVNEFYLLDGQ
HCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEEECCC
KFSTSRSHAVWGKEVLGQKTVDVVRLHLGLTRPEGERTNFTLDALRRTENEVFQGIWLNW
CCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDALHGEIKQDFGGCVPDAGDWAPADRAFFGRIEIHRAAMERAYSDDGFSVRRAAAQACD
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FVSDCVVYRRAQDYEAARRLYPARTRTSLALQTTAAAILAQTLAPLMPRFASTLARGIDG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VSTETWPTSVRRLAPGTTFDLGPVLGFYARADIPALNEEEA
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]