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The map label for this gene is nodE [H]

Identifier: 13474847

GI number: 13474847

Start: 4696717

End: 4697925

Strand: Direct

Name: nodE [H]

Synonym: mlr5822

Alternate gene names: 13474847

Gene position: 4696717-4697925 (Clockwise)

Preceding gene: 13474846

Following gene: 13474849

Centisome position: 66.75

GC content: 63.94

Gene sequence:

>1209_bases
ATTGGACAGGCGAGTCGTCATTACTGGGATAGGGGGGCTTTGCGGCTAGGCAACAACGCCGCCTCTATCTGGAAAGAGAT
GCGCGAAGGTCGCTCCGCCATCCGCCTGATGGTCACTTCCGCGCTTCATGAGATGAAGGGGACTGTCGGTACCGAGATCA
AGGAGCTGCCCCAGCACGACATCGGCCGCAACCAGCTCGTCTCCATGGACCGCCACAGCTTGCTCGCTGTGCTTGCAGCG
CGTGAAGCCATGCAGCACGCCGGACTTTCCTGCGATAAGGGAAATCCGTATCGCTTCGGCGCGACAGTGGGCGTCTGCGG
CTGGGGCTGGGGAACGGTCGAAGAAACCTACCGTGACCTCCTTTTGGGCGGCGCGACGCGTGCTGGCATTTACACTGCAC
CAAGGGGGTTGCCGAGCGCCGCTGCCAGCCAGGTCAGCATGAGTCTCGGCCTGCGCGGCCCGGTCTTTGCCATCGCCTCC
GCATGCGCATCAGCCAACCATGCGATCGCTTCGGCCGCGGATCAGATCAAGCTCGGCCGGGCCGACGTGATGCTTGCCGG
AGGCGCCGAAGCGCCGCTCGTATGCCTTCAGATGAAGGCATGGGAAGCAATGCGCGCGCTCGCTCCAGATACGTGCCGGC
CCTTCTCCGCCGACAGGAAGGGAGTGGTGATAGGCGAGGGTGCGGGCATGGCCGTGCTGGAAAGCTATGAGCATGCCGCC
GTGCGCGGTGCCACAATCCTTGCCGAGATCGCAGGCATCGGCCTTTCCGGCGACGCCTTCCACATCGTCGCGCCGTCTGT
CGAGGGGCCGGAGGCCGCGATCCGCGCTTGCCTTGCCGACGCCGGGCTGAATGCGGAGGACGTGGACTACCTAAACGCCC
ACGGCACCGGCACCAAGGCCAACGATCAGACTGAGACGGCGGCGATCAAGCGCGTCTTCGGAGACTATGCCTATTCGATG
TCTGTCTCGTCCACCAAGTCCATGTACGCGCATTGCCTGGGCGCAGCGAGCGCACTTGAAATGATCGCCTCCGTGATGGC
GATCCAGGAGGGCATTGTGCCGCCGACCGCGAACTATCGCGAGCCAGACCCCGATTGCGATCTCGACGTCACGCCCAATG
TGGCGCGCGAGCGCAAGGTGCGCGTGGCACTGAGCAACGCTTTCGCCATGGGCGGCATGAACGCAGTTCTGGCGTTCAAG
CAAGTGTAG

Upstream 100 bases:

>100_bases
GACATCAAGATCGACATGACGCGGCTGACGCCTGGTCGAATCTCAAGAATGTCGGCGACATGGTGGAAGCCGTCCGCGGT
TTGCTCGCGAAGGAGGTCTG

Downstream 100 bases:

>100_bases
AAGGCATGCGAGATGCCTCCAGTAACTCTATCTCTCGTTTCCAAATGCTTGCCGGATCACGATTGTGAAGGACGTGCCCT
TGGACCGCCTCGGTGAATGC

Product: nodulation protein nodE

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MGQASRHYWDRGALRLGNNAASIWKEMREGRSAIRLMVTSALHEMKGTVGTEIKELPQHDIGRNQLVSMDRHSLLAVLAA
REAMQHAGLSCDKGNPYRFGATVGVCGWGWGTVEETYRDLLLGGATRAGIYTAPRGLPSAAASQVSMSLGLRGPVFAIAS
ACASANHAIASAADQIKLGRADVMLAGGAEAPLVCLQMKAWEAMRALAPDTCRPFSADRKGVVIGEGAGMAVLESYEHAA
VRGATILAEIAGIGLSGDAFHIVAPSVEGPEAAIRACLADAGLNAEDVDYLNAHGTGTKANDQTETAAIKRVFGDYAYSM
SVSSTKSMYAHCLGAASALEMIASVMAIQEGIVPPTANYREPDPDCDLDVTPNVARERKVRVALSNAFAMGGMNAVLAFK
QV

Sequences:

>Translated_402_residues
MGQASRHYWDRGALRLGNNAASIWKEMREGRSAIRLMVTSALHEMKGTVGTEIKELPQHDIGRNQLVSMDRHSLLAVLAA
REAMQHAGLSCDKGNPYRFGATVGVCGWGWGTVEETYRDLLLGGATRAGIYTAPRGLPSAAASQVSMSLGLRGPVFAIAS
ACASANHAIASAADQIKLGRADVMLAGGAEAPLVCLQMKAWEAMRALAPDTCRPFSADRKGVVIGEGAGMAVLESYEHAA
VRGATILAEIAGIGLSGDAFHIVAPSVEGPEAAIRACLADAGLNAEDVDYLNAHGTGTKANDQTETAAIKRVFGDYAYSM
SVSSTKSMYAHCLGAASALEMIASVMAIQEGIVPPTANYREPDPDCDLDVTPNVARERKVRVALSNAFAMGGMNAVLAFK
QV
>Mature_401_residues
GQASRHYWDRGALRLGNNAASIWKEMREGRSAIRLMVTSALHEMKGTVGTEIKELPQHDIGRNQLVSMDRHSLLAVLAAR
EAMQHAGLSCDKGNPYRFGATVGVCGWGWGTVEETYRDLLLGGATRAGIYTAPRGLPSAAASQVSMSLGLRGPVFAIASA
CASANHAIASAADQIKLGRADVMLAGGAEAPLVCLQMKAWEAMRALAPDTCRPFSADRKGVVIGEGAGMAVLESYEHAAV
RGATILAEIAGIGLSGDAFHIVAPSVEGPEAAIRACLADAGLNAEDVDYLNAHGTGTKANDQTETAAIKRVFGDYAYSMS
VSSTKSMYAHCLGAASALEMIASVMAIQEGIVPPTANYREPDPDCDLDVTPNVARERKVRVALSNAFAMGGMNAVLAFKQ
V

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=401, Percent_Identity=35.9102244389027, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI223972627, Length=147, Percent_Identity=44.2176870748299, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI41872631, Length=366, Percent_Identity=26.775956284153, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1787337, Length=401, Percent_Identity=39.6508728179551, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1788663, Length=402, Percent_Identity=35.3233830845771, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71983541, Length=390, Percent_Identity=31.5384615384615, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71983535, Length=266, Percent_Identity=35.3383458646617, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI212642053, Length=320, Percent_Identity=27.1875, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17550940, Length=231, Percent_Identity=25.1082251082251, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320904, Length=355, Percent_Identity=33.2394366197183, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24644341, Length=401, Percent_Identity=38.6533665835411, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI221330659, Length=232, Percent_Identity=30.1724137931034, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19920632, Length=232, Percent_Identity=30.1724137931034, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581345, Length=279, Percent_Identity=25.089605734767, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 42219; Mature: 42087

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQASRHYWDRGALRLGNNAASIWKEMREGRSAIRLMVTSALHEMKGTVGTEIKELPQHD
CCCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCC
IGRNQLVSMDRHSLLAVLAAREAMQHAGLSCDKGNPYRFGATVGVCGWGWGTVEETYRDL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHH
LLGGATRAGIYTAPRGLPSAAASQVSMSLGLRGPVFAIASACASANHAIASAADQIKLGR
HHCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCC
ADVMLAGGAEAPLVCLQMKAWEAMRALAPDTCRPFSADRKGVVIGEGAGMAVLESYEHAA
CEEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VRGATILAEIAGIGLSGDAFHIVAPSVEGPEAAIRACLADAGLNAEDVDYLNAHGTGTKA
HHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NDQTETAAIKRVFGDYAYSMSVSSTKSMYAHCLGAASALEMIASVMAIQEGIVPPTANYR
CCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPDPDCDLDVTPNVARERKVRVALSNAFAMGGMNAVLAFKQV
CCCCCCCCCCCCCHHHHHHHEEHHHHHHHHCCHHHHHHEECC
>Mature Secondary Structure 
GQASRHYWDRGALRLGNNAASIWKEMREGRSAIRLMVTSALHEMKGTVGTEIKELPQHD
CCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCC
IGRNQLVSMDRHSLLAVLAAREAMQHAGLSCDKGNPYRFGATVGVCGWGWGTVEETYRDL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEECCCCCCCHHHHHHHH
LLGGATRAGIYTAPRGLPSAAASQVSMSLGLRGPVFAIASACASANHAIASAADQIKLGR
HHCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCC
ADVMLAGGAEAPLVCLQMKAWEAMRALAPDTCRPFSADRKGVVIGEGAGMAVLESYEHAA
CEEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VRGATILAEIAGIGLSGDAFHIVAPSVEGPEAAIRACLADAGLNAEDVDYLNAHGTGTKA
HHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
NDQTETAAIKRVFGDYAYSMSVSSTKSMYAHCLGAASALEMIASVMAIQEGIVPPTANYR
CCHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPDPDCDLDVTPNVARERKVRVALSNAFAMGGMNAVLAFKQV
CCCCCCCCCCCCCHHHHHHHEEHHHHHHHHCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2684629 [H]