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The map label for this gene is nuoF2 [H]

Identifier: 13474499

GI number: 13474499

Start: 4312198

End: 4313754

Strand: Reverse

Name: nuoF2 [H]

Synonym: mll5397

Alternate gene names: 13474499

Gene position: 4313754-4312198 (Counterclockwise)

Preceding gene: 13474500

Following gene: 13474498

Centisome position: 61.31

GC content: 64.93

Gene sequence:

>1557_bases
ATGATCCCCCGTATCTACATTCCCGGCGATTCCGGCGCGTTGGCGCTTGGCGCCGAAAAGGTCGCCAAGGCGATCGCCAA
GGAACTCGCGGAGCGTGGCATCGAGGCCAAGATCGTGCGCAACGGGTCGCGTGGCGCCTATTTCCTCGAGCCGATGGTGG
AAGTGGCGACCGCCAACGGCCGCGTCGCCTACGGACCGGTCAAGCCTTCCGACGTCAAGAGCCTGTTCGACAGCGGCTTC
CTCACCGGTGGCCACCACAAGCGCTGGCTCGGCGCGCCCGACAAGATCCCGTTCCTGGCCAAGCAGACGCGGCTGACCTT
TGCCCGCTGCGGCATCAACGACCCGCTGTCGCTCGACGCCTACAAGTCACTTGGCGGGCTCAAGGGCCTGCAGAACGCCG
TCGCCATGGCGCCGGCCGACATCGTCAAGCAGGTGACCGAGTCAGGCCTGCGTGGCCGTGGTGGCGCCGGCTTCCCGACC
GGCATCAAATGGAAGACGGTGCTGGATACGACATCCGAGCGCAAATACATCGTCTGCAACGCCGACGAGGGCGACAGCGC
GACTTTCGCCGACCGCATGATCATGGAAGGCGACCCCTTCGTGCTGATCGAAGGCATGGCGATCGCCGGCATCGCCACCG
GGGCGACCAAGGGCTTCGTTTATATCCGCTCGGAATATCCGCATGCCGTGGCGACAATGAACAAGGCCGTCGAGATCGCC
CGCAAGGCCGGCGTCCTCGGCGTCAACGTGCTGGGTTCGCCCAATGCCTTCGACATGGAAATCCGCGTCGGCGCCGGCGC
CTATGTCTGCGGCGAAGAAACCTCGCTGTTGAACAGCCTGGAAGGCAAACGCGGCGTGGTGCGCGCCAAGCCGCCGCTGC
CGGCCATTCAGGGCCTGTTCGGCAAGCCGACGGTGATCAACAACGTCATCAGCCTGGCCTCGGTGCCGATCATCATGGAC
AAGGGCGCCGCCTTCTACAAGGATTTCGGCATGGGCCGCTCGCGCGGCACGATCCCGATCCAGATCGCCGGCAATGTCAG
GAACGGCGGCCTGTTCGAGACCGCCTTCGGCCTGACGCTGGGCGAGATCGTCGATGACATCGGCGGCGGCACGGCGACTG
GCCGTCCAGTGAAGGCAGTACAGGTCGGCGGTCCGCTTGGCGCCTATTTCCCGCGCGCCCTGTTCGACACGCCGTTCGAC
TACGAGGAATTCGCCAAGCGCGACGGGCTGATCGGCCATGCCGGCATCACCGTGTTCGACGACACGGCCGACATGCTGAA
GCAGGCGCGCTTCGCCATGGAGTTCTGCGCCATCGAAAGCTGCGGCAAGTGCACGCCCTGCCGCATCGGCTCGACGCGCG
GCATTGAGACCATCGACAGGCTTGCCGCCGGCATCGAGCCGGAAAAGAACCTCGCTCTGGTCACCGACCTCTGCAACACG
ATGAAGTTCGGTTCGCTGTGCGCACTGGGCGGCTTCACGCCTTATCCGGTGATGAGTTCGATCACGCATTTCCCCGAAGA
TTTCAAGCCCGCGCCAGCGCGCGTGGCTGCTGAATAG

Upstream 100 bases:

>100_bases
TCGGGCTTTGCGCCTGTTCGCCGTCGGCGATGCTCGACGGCGAGGTGATCGGGCGGCTCGATGACGACAAGATCGACGAG
ATCCTTGCCGAGGTGCGCTC

Downstream 100 bases:

>100_bases
GAGCTGGCAGATGAACATCAAGGCCGACTTCCCATCGCTCGTCGAAGAGATCGACTACGGAACCCCGGAATCGCGGGCGC
AAAAGCAGGTCACGCTGAAC

Product: NAD-dependent formate dehydrogenase beta subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MIPRIYIPGDSGALALGAEKVAKAIAKELAERGIEAKIVRNGSRGAYFLEPMVEVATANGRVAYGPVKPSDVKSLFDSGF
LTGGHHKRWLGAPDKIPFLAKQTRLTFARCGINDPLSLDAYKSLGGLKGLQNAVAMAPADIVKQVTESGLRGRGGAGFPT
GIKWKTVLDTTSERKYIVCNADEGDSATFADRMIMEGDPFVLIEGMAIAGIATGATKGFVYIRSEYPHAVATMNKAVEIA
RKAGVLGVNVLGSPNAFDMEIRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPAIQGLFGKPTVINNVISLASVPIIMD
KGAAFYKDFGMGRSRGTIPIQIAGNVRNGGLFETAFGLTLGEIVDDIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFD
YEEFAKRDGLIGHAGITVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSTRGIETIDRLAAGIEPEKNLALVTDLCNT
MKFGSLCALGGFTPYPVMSSITHFPEDFKPAPARVAAE

Sequences:

>Translated_518_residues
MIPRIYIPGDSGALALGAEKVAKAIAKELAERGIEAKIVRNGSRGAYFLEPMVEVATANGRVAYGPVKPSDVKSLFDSGF
LTGGHHKRWLGAPDKIPFLAKQTRLTFARCGINDPLSLDAYKSLGGLKGLQNAVAMAPADIVKQVTESGLRGRGGAGFPT
GIKWKTVLDTTSERKYIVCNADEGDSATFADRMIMEGDPFVLIEGMAIAGIATGATKGFVYIRSEYPHAVATMNKAVEIA
RKAGVLGVNVLGSPNAFDMEIRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPAIQGLFGKPTVINNVISLASVPIIMD
KGAAFYKDFGMGRSRGTIPIQIAGNVRNGGLFETAFGLTLGEIVDDIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFD
YEEFAKRDGLIGHAGITVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSTRGIETIDRLAAGIEPEKNLALVTDLCNT
MKFGSLCALGGFTPYPVMSSITHFPEDFKPAPARVAAE
>Mature_518_residues
MIPRIYIPGDSGALALGAEKVAKAIAKELAERGIEAKIVRNGSRGAYFLEPMVEVATANGRVAYGPVKPSDVKSLFDSGF
LTGGHHKRWLGAPDKIPFLAKQTRLTFARCGINDPLSLDAYKSLGGLKGLQNAVAMAPADIVKQVTESGLRGRGGAGFPT
GIKWKTVLDTTSERKYIVCNADEGDSATFADRMIMEGDPFVLIEGMAIAGIATGATKGFVYIRSEYPHAVATMNKAVEIA
RKAGVLGVNVLGSPNAFDMEIRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPAIQGLFGKPTVINNVISLASVPIIMD
KGAAFYKDFGMGRSRGTIPIQIAGNVRNGGLFETAFGLTLGEIVDDIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFD
YEEFAKRDGLIGHAGITVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSTRGIETIDRLAAGIEPEKNLALVTDLCNT
MKFGSLCALGGFTPYPVMSSITHFPEDFKPAPARVAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=421, Percent_Identity=35.6294536817102, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI20149568, Length=421, Percent_Identity=35.6294536817102, Blast_Score=244, Evalue=2e-64,
Organism=Escherichia coli, GI1788620, Length=408, Percent_Identity=38.2352941176471, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17531727, Length=382, Percent_Identity=38.4816753926702, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19920794, Length=370, Percent_Identity=38.1081081081081, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI45550452, Length=390, Percent_Identity=35.8974358974359, Blast_Score=227, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24653819, Length=361, Percent_Identity=30.1939058171745, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=27.4096385542169, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54754; Mature: 54754

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRIYIPGDSGALALGAEKVAKAIAKELAERGIEAKIVRNGSRGAYFLEPMVEVATANG
CCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHCCCC
RVAYGPVKPSDVKSLFDSGFLTGGHHKRWLGAPDKIPFLAKQTRLTFARCGINDPLSLDA
EEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCHHH
YKSLGGLKGLQNAVAMAPADIVKQVTESGLRGRGGAGFPTGIKWKTVLDTTSERKYIVCN
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSATFADRMIMEGDPFVLIEGMAIAGIATGATKGFVYIRSEYPHAVATMNKAVEIA
CCCCCCCHHHHHHEECCCCEEEEECCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHH
RKAGVLGVNVLGSPNAFDMEIRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPAIQGLF
HHCCEEEEEEECCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCC
GKPTVINNVISLASVPIIMDKGAAFYKDFGMGRSRGTIPIQIAGNVRNGGLFETAFGLTL
CCCHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
GEIVDDIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFDYEEFAKRDGLIGHAGITVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCEEECCEEEEC
DTADMLKQARFAMEFCAIESCGKCTPCRIGSTRGIETIDRLAAGIEPEKNLALVTDLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
MKFGSLCALGGFTPYPVMSSITHFPEDFKPAPARVAAE
HHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIPRIYIPGDSGALALGAEKVAKAIAKELAERGIEAKIVRNGSRGAYFLEPMVEVATANG
CCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHCCCC
RVAYGPVKPSDVKSLFDSGFLTGGHHKRWLGAPDKIPFLAKQTRLTFARCGINDPLSLDA
EEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEECCCCCCCCHHH
YKSLGGLKGLQNAVAMAPADIVKQVTESGLRGRGGAGFPTGIKWKTVLDTTSERKYIVCN
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSATFADRMIMEGDPFVLIEGMAIAGIATGATKGFVYIRSEYPHAVATMNKAVEIA
CCCCCCCHHHHHHEECCCCEEEEECCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHH
RKAGVLGVNVLGSPNAFDMEIRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPAIQGLF
HHCCEEEEEEECCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCC
GKPTVINNVISLASVPIIMDKGAAFYKDFGMGRSRGTIPIQIAGNVRNGGLFETAFGLTL
CCCHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCEEEEEEECCCCCCCEEHHHHHHHH
GEIVDDIGGGTATGRPVKAVQVGGPLGAYFPRALFDTPFDYEEFAKRDGLIGHAGITVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCEEECCEEEEC
DTADMLKQARFAMEFCAIESCGKCTPCRIGSTRGIETIDRLAAGIEPEKNLALVTDLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHH
MKFGSLCALGGFTPYPVMSSITHFPEDFKPAPARVAAE
HHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]