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The map label for this gene is ptsI [H]

Identifier: 13474413

GI number: 13474413

Start: 4219827

End: 4221410

Strand: Reverse

Name: ptsI [H]

Synonym: mll5291

Alternate gene names: 13474413

Gene position: 4221410-4219827 (Counterclockwise)

Preceding gene: 13474414

Following gene: 13474412

Centisome position: 60.0

GC content: 67.05

Gene sequence:

>1584_bases
ATGCGGGCCATGCGGATTGAGGGTGTTCCGGCCTCGGCCGGCTATGCCGAGGGGCCGCTGTTCGACCTCGACCGGCCGCC
GGCCGTCTACGCAGGCAAAGCCACATTAGCGGAAGAGAAGACGTCGCTGCAAGCCGCGATCGGCACGGCAGTGAGCCGGC
TTTCCGCCATGGCGGAGGCAGCCGACAGCGACGCCGCCGGCATACTCGAATTCCACATTGCCATGCTGGAAGACGACGCC
TTGAGAGGGCCGGCCTTCGCGTCGATCACGTCCGGACAGACCGCCGACGTGGCATGGCGAGAGGCGCTCGATGCCGAGAT
CGCCGGTTACGAGGCCTCCGACCAGGACTATTTCCGTGCCCGCGCCACGGACCTGCGCGATATCAGGGACCAAGTGCTGC
GTGCCCTGACCGAGGACGTCGACAGCGCGGCGCCTACCGGTGCCATCCTCTGTGGCGAGGACATCGCGCCGACGCGCTTT
CTCGAAACCGACTGGAGCCATGGCGGCGGCATCGCGCTGAAGGCGGGCAGCACCGCCAGCCACGTCGCCATGCTGGCGCG
CTCACGTGGCGTGCCTATGGTCGTGGGTCTGCGCGAAATGTCTGCCAGTCCTGCCGGCATGGCACTGCTCGACGCCGAGC
ATGGCAGCATCGTGCTGGCGCCCTCGCCGGCGGAGATCGGCGCGTTCCGGCAGTCATCCGCTTCCTTCGCGGCCCGTCAG
GGCAAGGCGCAGCGTTTTCTGGCGCGGCCGGCGGTGACCAAGGCCGGCACGGCGGTGCGCGTGCAAGTCAACATCGCCGA
CCCATCCGATGTCGACGGCATTGACGTCGCGACCTGCGACGGGGTCGGGCTGATGCGAACAGAGTTCTTGTTCGGCAAGA
ATTTGCCGGATGAGGAGACGCAATACCGCGCCTATCGCAAGGTGCTGGAATGGGCCGGGGAAAAACCGGTGACGATCCGT
ACTGTCGATGCGGGCGGCGACAAGCCGGTGCCGGGCTTCACCGTCGAGGAAGGCAATCCATTCCTTGGCCTGCGCGGGAT
CAGGCTGTCGCTGGCGCGGCTCGACATTTTTCGCATACAGATCAGGGCATTGCTGCGCGCCGCGCCACACGGCAATCTCA
GAGTGATGTTTCCGATGATCGCCATGGCCGACGAGTATGAGCGCGCAGCCGCCCTGTTCGCCGAAGAGCAGGCAGCCCTT
GCCGCGGGCGGTGTCGCGCAAAAGATGCCGCCGCTGGGCATCATGGTCGAGGTGCCGTCGGTGGCCATCGCGCCCGAGGC
CTTCGCCGGCGTGGCCTTCTTCTCGATCGGCTCCAACGATCTGACGCAATATGTGATGGCGGCCGCGCGGGATAACGCTG
CTGTGGCCCATTTCAATTCGGTCCGCCACCCGGCGGTGCTGCGGCTGATCGGCTCGGTCGCCGCCTTTGGACGGGAAAAC
GGCATTCCCGTCAGCCTGTGCGGCGATGCCGGCGGCGATCCGGCAGCGATCCCGTCGCTGCTCGAAGCGGGCCTGCGCGA
TCTGTCCGTCGCGCCGGCGCAACTCGCCATGGCCAAGGCGGCCATCGCGGATGTTTCGGTCTAG

Upstream 100 bases:

>100_bases
TACGCGCGCTGATCGACCTGGTGCAGCGAGATTTCGACGAGGACGCCGGTCATGTCCGCTCGGCGTGAGGCGGGCGCGCT
TCTTCGCCGGCTCGCGGGGA

Downstream 100 bases:

>100_bases
GCGCCCGGCATGGTCGAGATCAAACCCGAACCCGGCTCCGAAGAGGCGATCCGCGCCTACAAGACGATCCTGTCGCAGGT
CATCGACCAGCGCCCGTCCG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRAMRIEGVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEAADSDAAGILEFHIAMLEDDA
LRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRARATDLRDIRDQVLRALTEDVDSAAPTGAILCGEDIAPTRF
LETDWSHGGGIALKAGSTASHVAMLARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQ
GKAQRFLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLFGKNLPDEETQYRAYRKVLEWAGEKPVTIR
TVDAGGDKPVPGFTVEEGNPFLGLRGIRLSLARLDIFRIQIRALLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAAL
AAGGVAQKMPPLGIMVEVPSVAIAPEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGREN
GIPVSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVSV

Sequences:

>Translated_527_residues
MRAMRIEGVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEAADSDAAGILEFHIAMLEDDA
LRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRARATDLRDIRDQVLRALTEDVDSAAPTGAILCGEDIAPTRF
LETDWSHGGGIALKAGSTASHVAMLARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQ
GKAQRFLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLFGKNLPDEETQYRAYRKVLEWAGEKPVTIR
TVDAGGDKPVPGFTVEEGNPFLGLRGIRLSLARLDIFRIQIRALLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAAL
AAGGVAQKMPPLGIMVEVPSVAIAPEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGREN
GIPVSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVSV
>Mature_527_residues
MRAMRIEGVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEAADSDAAGILEFHIAMLEDDA
LRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRARATDLRDIRDQVLRALTEDVDSAAPTGAILCGEDIAPTRF
LETDWSHGGGIALKAGSTASHVAMLARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQ
GKAQRFLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLFGKNLPDEETQYRAYRKVLEWAGEKPVTIR
TVDAGGDKPVPGFTVEEGNPFLGLRGIRLSLARLDIFRIQIRALLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAAL
AAGGVAQKMPPLGIMVEVPSVAIAPEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGREN
GIPVSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVSV

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=547, Percent_Identity=31.9926873857404, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788756, Length=446, Percent_Identity=34.9775784753363, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788726, Length=471, Percent_Identity=32.9087048832272, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI48994992, Length=504, Percent_Identity=32.1428571428571, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787994, Length=428, Percent_Identity=23.8317757009346, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI226510935, Length=166, Percent_Identity=33.1325301204819, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 55119; Mature: 55119

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMRIEGVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEA
CCCEEECCCCCCCCCCCCCEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSDAAGILEFHIAMLEDDALRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRA
CCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
RATDLRDIRDQVLRALTEDVDSAAPTGAILCGEDIAPTRFLETDWSHGGGIALKAGSTAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCEEEECCCCHH
HVAMLARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQ
HHHHHHHCCCCEEEEEHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCHHHHHHHH
GKAQRFLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLFGKNLPDEET
CHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCHHH
QYRAYRKVLEWAGEKPVTIRTVDAGGDKPVPGFTVEEGNPFLGLRGIRLSLARLDIFRIQ
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEEHHEEEHHHHHHHHHH
IRALLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAALAAGGVAQKMPPLGIMVEVPS
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCC
VAIAPEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGREN
EEECCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCC
GIPVSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVSV
CCEEEECCCCCCCHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAMRIEGVPASAGYAEGPLFDLDRPPAVYAGKATLAEEKTSLQAAIGTAVSRLSAMAEA
CCCEEECCCCCCCCCCCCCEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADSDAAGILEFHIAMLEDDALRGPAFASITSGQTADVAWREALDAEIAGYEASDQDYFRA
CCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
RATDLRDIRDQVLRALTEDVDSAAPTGAILCGEDIAPTRFLETDWSHGGGIALKAGSTAS
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCEEEECCCCHH
HVAMLARSRGVPMVVGLREMSASPAGMALLDAEHGSIVLAPSPAEIGAFRQSSASFAARQ
HHHHHHHCCCCEEEEEHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCHHHHHHHH
GKAQRFLARPAVTKAGTAVRVQVNIADPSDVDGIDVATCDGVGLMRTEFLFGKNLPDEET
CHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCHHH
QYRAYRKVLEWAGEKPVTIRTVDAGGDKPVPGFTVEEGNPFLGLRGIRLSLARLDIFRIQ
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEEHHEEEHHHHHHHHHH
IRALLRAAPHGNLRVMFPMIAMADEYERAAALFAEEQAALAAGGVAQKMPPLGIMVEVPS
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCC
VAIAPEAFAGVAFFSIGSNDLTQYVMAAARDNAAVAHFNSVRHPAVLRLIGSVAAFGREN
EEECCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHCCCC
GIPVSLCGDAGGDPAAIPSLLEAGLRDLSVAPAQLAMAKAAIADVSV
CCEEEECCCCCCCHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]