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The map label for this gene is gyrB [H]

Identifier: 13474326

GI number: 13474326

Start: 4120221

End: 4122692

Strand: Reverse

Name: gyrB [H]

Synonym: mll5188

Alternate gene names: 13474326

Gene position: 4122692-4120221 (Counterclockwise)

Preceding gene: 13474327

Following gene: 13474325

Centisome position: 58.59

GC content: 63.63

Gene sequence:

>2472_bases
ATGAGCGACCAGATCGAGAACACCAACGGCGCCGAAGCCGAATACGGCGCCGATTCCATTAAGGTTTTGAAGGGGTTGGA
CGCTGTCCGCAAACGCCCTGGCATGTATATCGGCGACACGGACGACGGTTCGGGCCTGCATCACATGGTCTACGAGGTGG
TCGACAACGCCATCGACGAGGCCCTGGCAGGGCACGCCGACCTCGTCACGGTGACGCTCAACCCCGATGGTTCGGTGACG
GTGATCGACAATGGCCGTGGCATTCCGACCGATATTCACACCGGCGAAGGCATTTCCGCGGCCGAAGTGATCATGACGCA
GCTGCATGCCGGGGGCAAATTCGACCAGAACTCCTACAAGGTGTCGGGCGGCCTGCATGGCGTCGGCGTCTCGGTGGTCA
ACGCGCTTTCGGCCTGGCTGAAGCTCAAGATTCGCCGCAACGGCCAGATCTTCGAAATGAGCTTCACGCACGGCAATGCC
GATGCGCCGTTGAGGGCGACCGGTAGCTACGAGCAGGACAAGCAGCCGGGCACCTATGAGGGCCGCAGCGGTAGCTCTAT
CACCTTCTTCCCGTCGGCCGAGACCTTCACCATGGTCGAATTCGACTTCGGCACGCTGGAGCACCGGCTGCGCGAGCTCG
CCTTCCTCAATTCCGGCGTGCGCATCATTCTGACCGACGGCCGCCACGCCGACATCGTGCGGCATGAACTGCACTATGAT
GGCGGTCTCGAGGAGTTCGTCAAATATCTCGACCGGGTCAAGAAGCCGCTGATCGAGAAGCCGATCGCCATCAAGGCCGA
GCGCGACGGCATCACTGTCGAAGTGGCGATGTGGTGGAACGACAGCTACCACGAGAACGTGCTGGCCTTCACCAACAACA
TCCCGCAGCGCGATGGCGGCACCCATCTGGCCGGTTTCCGTGGCGCGCTGACGCGCCAGATCACCGGCTATGGCGAGTCC
TCGGGCCTGACCAAGAAGGAAAAGGTCTCGCTGATCGGCGACGATTGCCGCGAAGGGCTGACGGCGGTGCTGTCGGTCAA
GGTTCCCGATCCGAAATTCTCCTCGCAGACCAAGGACAAGCTGGTTTCCTCCGAGGTCCGCCCGGTGGTCGAGGGGCTGG
TCAACGAGGCGCTCGGCACCTGGCTGGAAGAGCATCCGACCGAAGGCAAGGTGGTCATCGACAAGGTGATCCAGGCCGCG
GCGGCGCGCGAAGCCGCGCGCAAGGCGCGCGACATCACCCGCAAGAGCTCGCTCGGCGTCACGTCCTTGCCCGGCAAGCT
GGCCGACTGCCAGGAGCGCGATCCGGCGAAATCCGAAATCTTCATCGTCGAGGGTGACTCGGCCGGCGGCTCGGCCAAGG
GCGGCCGTTCGCGCCAGAACCAGGCCATCCTTCCCCTGCGCGGCAAAATCCTCAATGTCGAGCGGGCGCGTTTCGACCGC
ATGCTCGGCTCCGACATGATCGGCACGCTGATCACCGCGCTCGGCACCTCGATCGGCAAGGACGAGTTCAACGCCGACAA
GCTGCGCTACCACAAGATCATCCTGATGACCGACGCCGACGTCGACGGCGCCCATATCAGAACCTTGCTGCTCACCTTCT
TCTTCCGGCAGATGCCCGAGCTGATCGAGCGCGGCCATCTCTACATCGCTCAGCCGCCGCTCTACAAAGTGACGCGCGGC
AAGAGCTCGCAATACATCAAGAATGAAAGCGCCTTCGAGGAGTTCCTGATCGAGTCCGGGCTGGAGGAAGCTTCGCTGAC
GCTGGGCTCGGGGGAAGTGCGGACCGGGCAGGATCTGCGCGGTGCGGTCGACGACGCGCTGGCTGTGCGCCAGCTTATCA
ACGGCCTGCACACGCGCTACAACAGAGGCGTGGTCGAACAAGCGTCGATCGCCGGTGGGCTCAACCCCGAAGTCTTCAGC
GATCTTGGCCGTGCCAACGCCATGGCGGAGCGGATTGCACAGCGCCTCGACATCATCGCCGAGGACACCGAGCGCGGCTG
GACCGGGCGCATGTCGACCTCGAATGACGGCAGCGGGGGCTATGTCTTCGAGCGCACCGTGCGCAGCGTCAAGGAATACG
CCCATCTCGACATGGGGCTGATCAATTCGGCCGACGCCCGCCAGCTCGACCGCTACGCGCAGCGCCTCAGCGAGGTCTAC
GGGACGCCGCCGGTGCTGCGCCGCAAGGATGTGTCCGAAACCGTCTCGGGGCCGCTGGCGCTGCTCAACGCCGTCTTTGC
GACAGGTCGCAAGGGGCTGACCATGCAGCGCTACAAGGGTCTCGGCGAGATGAATGCCGAGCAATTGTGGGAAACCACGC
TGGACCCGAATGTGCGGTCGCTGCTGCAGGTCAAGGTCAACGATGCGACCGATGCCGACAGCCTGTTCTCGCGGCTGATG
GGCGACGAGGTCGAGCCACGGCGCGAATTCATCCAGGACAACGCGCTGTCGGTCGCCAATCTGGATATTTGA

Upstream 100 bases:

>100_bases
GAAAAACCGCCTTTAGCGCCTATATCTAACGCTATATCAGGCGCGCGATTCGGGGCTGATTTTTCGCGCCGTACAAGCGG
CATCTAGACAATAGGTTTTC

Downstream 100 bases:

>100_bases
TGCATGTTGCCCAAAACTGCGCAGCGGTTTTGGGCGAACAATATGCACAAGAACACGCACCTAAGCGCGTGGCTGAATCC
GTTTCGACGCGACGCGCTTT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 823; Mature: 822

Protein sequence:

>823_residues
MSDQIENTNGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNPDGSVT
VIDNGRGIPTDIHTGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSAWLKLKIRRNGQIFEMSFTHGNA
DAPLRATGSYEQDKQPGTYEGRSGSSITFFPSAETFTMVEFDFGTLEHRLRELAFLNSGVRIILTDGRHADIVRHELHYD
GGLEEFVKYLDRVKKPLIEKPIAIKAERDGITVEVAMWWNDSYHENVLAFTNNIPQRDGGTHLAGFRGALTRQITGYGES
SGLTKKEKVSLIGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALGTWLEEHPTEGKVVIDKVIQAA
AAREAARKARDITRKSSLGVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKGGRSRQNQAILPLRGKILNVERARFDR
MLGSDMIGTLITALGTSIGKDEFNADKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRG
KSSQYIKNESAFEEFLIESGLEEASLTLGSGEVRTGQDLRGAVDDALAVRQLINGLHTRYNRGVVEQASIAGGLNPEVFS
DLGRANAMAERIAQRLDIIAEDTERGWTGRMSTSNDGSGGYVFERTVRSVKEYAHLDMGLINSADARQLDRYAQRLSEVY
GTPPVLRRKDVSETVSGPLALLNAVFATGRKGLTMQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLM
GDEVEPRREFIQDNALSVANLDI

Sequences:

>Translated_823_residues
MSDQIENTNGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNPDGSVT
VIDNGRGIPTDIHTGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSAWLKLKIRRNGQIFEMSFTHGNA
DAPLRATGSYEQDKQPGTYEGRSGSSITFFPSAETFTMVEFDFGTLEHRLRELAFLNSGVRIILTDGRHADIVRHELHYD
GGLEEFVKYLDRVKKPLIEKPIAIKAERDGITVEVAMWWNDSYHENVLAFTNNIPQRDGGTHLAGFRGALTRQITGYGES
SGLTKKEKVSLIGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALGTWLEEHPTEGKVVIDKVIQAA
AAREAARKARDITRKSSLGVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKGGRSRQNQAILPLRGKILNVERARFDR
MLGSDMIGTLITALGTSIGKDEFNADKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRG
KSSQYIKNESAFEEFLIESGLEEASLTLGSGEVRTGQDLRGAVDDALAVRQLINGLHTRYNRGVVEQASIAGGLNPEVFS
DLGRANAMAERIAQRLDIIAEDTERGWTGRMSTSNDGSGGYVFERTVRSVKEYAHLDMGLINSADARQLDRYAQRLSEVY
GTPPVLRRKDVSETVSGPLALLNAVFATGRKGLTMQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLM
GDEVEPRREFIQDNALSVANLDI
>Mature_822_residues
SDQIENTNGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHADLVTVTLNPDGSVTV
IDNGRGIPTDIHTGEGISAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSAWLKLKIRRNGQIFEMSFTHGNAD
APLRATGSYEQDKQPGTYEGRSGSSITFFPSAETFTMVEFDFGTLEHRLRELAFLNSGVRIILTDGRHADIVRHELHYDG
GLEEFVKYLDRVKKPLIEKPIAIKAERDGITVEVAMWWNDSYHENVLAFTNNIPQRDGGTHLAGFRGALTRQITGYGESS
GLTKKEKVSLIGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALGTWLEEHPTEGKVVIDKVIQAAA
AREAARKARDITRKSSLGVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKGGRSRQNQAILPLRGKILNVERARFDRM
LGSDMIGTLITALGTSIGKDEFNADKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPELIERGHLYIAQPPLYKVTRGK
SSQYIKNESAFEEFLIESGLEEASLTLGSGEVRTGQDLRGAVDDALAVRQLINGLHTRYNRGVVEQASIAGGLNPEVFSD
LGRANAMAERIAQRLDIIAEDTERGWTGRMSTSNDGSGGYVFERTVRSVKEYAHLDMGLINSADARQLDRYAQRLSEVYG
TPPVLRRKDVSETVSGPLALLNAVFATGRKGLTMQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLMG
DEVEPRREFIQDNALSVANLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=574, Percent_Identity=24.390243902439, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI19913408, Length=568, Percent_Identity=23.5915492957746, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI48994957, Length=816, Percent_Identity=55.1470588235294, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=563, Percent_Identity=38.7211367673179, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=544, Percent_Identity=23.1617647058824, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=533, Percent_Identity=22.5140712945591, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=141, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=611, Percent_Identity=27.1685761047463, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136538, Length=547, Percent_Identity=25.7769652650823, Blast_Score=136, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90106; Mature: 89975

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIENTNGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
ALAGHADLVTVTLNPDGSVTVIDNGRGIPTDIHTGEGISAAEVIMTQLHAGGKFDQNSYK
HHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSAWLKLKIRRNGQIFEMSFTHGNADAPLRATGSYEQDKQPGTYE
ECCCCCHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCC
GRSGSSITFFPSAETFTMVEFDFGTLEHRLRELAFLNSGVRIILTDGRHADIVRHELHYD
CCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GGLEEFVKYLDRVKKPLIEKPIAIKAERDGITVEVAMWWNDSYHENVLAFTNNIPQRDGG
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCHHEEECCCCCCCCC
THLAGFRGALTRQITGYGESSGLTKKEKVSLIGDDCREGLTAVLSVKVPDPKFSSQTKDK
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
LVSSEVRPVVEGLVNEALGTWLEEHPTEGKVVIDKVIQAAAAREAARKARDITRKSSLGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKGGRSRQNQAILPLRGKILNVERARFDR
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHH
MLGSDMIGTLITALGTSIGKDEFNADKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPE
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LIERGHLYIAQPPLYKVTRGKSSQYIKNESAFEEFLIESGLEEASLTLGSGEVRTGQDLR
HHHCCCEEEECCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCHHEEEECCCCCCCCCHHH
GAVDDALAVRQLINGLHTRYNRGVVEQASIAGGLNPEVFSDLGRANAMAERIAQRLDIIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDTERGWTGRMSTSNDGSGGYVFERTVRSVKEYAHLDMGLINSADARQLDRYAQRLSEVY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GTPPVLRRKDVSETVSGPLALLNAVFATGRKGLTMQRYKGLGEMNAEQLWETTLDPNVRS
CCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHH
LLQVKVNDATDADSLFSRLMGDEVEPRREFIQDNALSVANLDI
HEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
SDQIENTNGAEAEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
ALAGHADLVTVTLNPDGSVTVIDNGRGIPTDIHTGEGISAAEVIMTQLHAGGKFDQNSYK
HHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGVSVVNALSAWLKLKIRRNGQIFEMSFTHGNADAPLRATGSYEQDKQPGTYE
ECCCCCHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCC
GRSGSSITFFPSAETFTMVEFDFGTLEHRLRELAFLNSGVRIILTDGRHADIVRHELHYD
CCCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCC
GGLEEFVKYLDRVKKPLIEKPIAIKAERDGITVEVAMWWNDSYHENVLAFTNNIPQRDGG
CCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCCCCHHEEECCCCCCCCC
THLAGFRGALTRQITGYGESSGLTKKEKVSLIGDDCREGLTAVLSVKVPDPKFSSQTKDK
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
LVSSEVRPVVEGLVNEALGTWLEEHPTEGKVVIDKVIQAAAAREAARKARDITRKSSLGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKGGRSRQNQAILPLRGKILNVERARFDR
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHH
MLGSDMIGTLITALGTSIGKDEFNADKLRYHKIILMTDADVDGAHIRTLLLTFFFRQMPE
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LIERGHLYIAQPPLYKVTRGKSSQYIKNESAFEEFLIESGLEEASLTLGSGEVRTGQDLR
HHHCCCEEEECCCCEEECCCCCCHHHCCHHHHHHHHHHCCCCHHEEEECCCCCCCCCHHH
GAVDDALAVRQLINGLHTRYNRGVVEQASIAGGLNPEVFSDLGRANAMAERIAQRLDIIA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDTERGWTGRMSTSNDGSGGYVFERTVRSVKEYAHLDMGLINSADARQLDRYAQRLSEVY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
GTPPVLRRKDVSETVSGPLALLNAVFATGRKGLTMQRYKGLGEMNAEQLWETTLDPNVRS
CCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHH
LLQVKVNDATDADSLFSRLMGDEVEPRREFIQDNALSVANLDI
HEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]