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The map label for this gene is hcaE [H]

Identifier: 13474255

GI number: 13474255

Start: 4043034

End: 4044281

Strand: Reverse

Name: hcaE [H]

Synonym: mll5101

Alternate gene names: 13474255

Gene position: 4044281-4043034 (Counterclockwise)

Preceding gene: 13474260

Following gene: 13474254

Centisome position: 57.48

GC content: 62.42

Gene sequence:

>1248_bases
ATGGATGCGCGCAACGATATGCTGAGGCTCTTGAATGGCCGCAGAGAGGGCTTCTCGCTCGAGCAGCCCTTCTATACCGA
TCCCGACTTCTTCAAGCTCGACATGGAGCTGATCTGGTATCGCGACTGGCTGTTCGTCGGCCATGATTGCGAACTGCCCA
AGCCCGGCAGCTTCATCACCGTGCAGATCGGCGACTATCCGGTCGTGCTGGTGCGCGATCAGCAGGGCAGGATCAACGCA
TTCCACAATTCCTGCCGCCATCGCGGCAGCCGTGTCTGCAACACCGACAAGGGCACGGCCGCCAAGCTGGTCTGCCCCTA
CCACCAGTGGACCTATGAGCTGGATGGAAGGCTGTTGTTTGCCCGCCAGATGGCCGACGGTTTCGACAAGAGCCAGTTCG
GCCTGAAGCCGGTCGCCTGCGAAAGCGTTGCCGGCTATATCTTCATCTGCCTGGCCAAGGAACCCGCCGACTTCGCCCCG
ATGCGGGCGATGATCGAACCTTATCTCAAGCCGCACCGGCTCAGCGAAGCAAAGATCGCCTTCGAGAGCACCATCGTCGA
GAAGGGCAACTGGAAGCTCGTCTGGGAAAACAACCGCGAGTGCTACCATTGCGCCGGCAATCACCCTGAACTGTGCAAGA
CCTTCCCGGAAGCCCCGACGGTGACCGGCGTGCAAGGCGCCGACAGCGATCCGGAGATGCTGGCACACTGGGCCAAATGC
GAGGCGGCCGGCCTGCCGAGCAAGTTCCGCATCGACCCGGCGGGGCAATACCGCGCCACCCGCGCGCCGCTGCTGCGCGA
CGCGGTCAGCTACACGATGACCGGCAAGCGGGCGGTGAAGAAGAACCTGTCCGACAGCGTCTCGGCCGACCGCATCGGCA
CGTTGATGCACTATCACTATCCGACGACCTGGAACCATATCCTCATCGATCATGCCGTCACCTTCCGCGTGCTGCCGATC
AGCGCCACGGAAACCGCGGTGACGACCAAATGGCTGGTGCACAAGGATGCGGTCGAGGGCGTCGACTACGATCTTGCCGA
ACTCACCCATGTCTGGACCGAGACCAACGACCAGGATCGCCGCATCGTCGAGGAAAACGCCTTCGGCATTCTGTCGCCGG
CCTATGAACCCGGTCCCTATTCGGAACTGCATGAGGGCGGCGTGATCCAGTTCGTCGACTGGTATGCCCGCTTCATCGGT
CCGCGCCTTGCCGGAGATGGCCGGCCGGCGTTGCGCAGCGTTGCTTGA

Upstream 100 bases:

>100_bases
GCGGTCAAGAGGCGGATGGCGCTTGCCCGGCTATCGCCGGGCTACGTCTAATCCTTCCTCAATCACCACCAGTTCAGTCC
TTGAAGTATGAGGACAGGAC

Downstream 100 bases:

>100_bases
GAGGTTGAGGGATGAATGCGATGACGGATCTTGGCCTCTATCGCCATCTCGACCAGATGGCGCCCTGGAACGACAGGCTG
CAGGTGCTGGAAGTGATCGG

Product: aromatic-ring hydroxylating dioxygenase alpha-subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDARNDMLRLLNGRREGFSLEQPFYTDPDFFKLDMELIWYRDWLFVGHDCELPKPGSFITVQIGDYPVVLVRDQQGRINA
FHNSCRHRGSRVCNTDKGTAAKLVCPYHQWTYELDGRLLFARQMADGFDKSQFGLKPVACESVAGYIFICLAKEPADFAP
MRAMIEPYLKPHRLSEAKIAFESTIVEKGNWKLVWENNRECYHCAGNHPELCKTFPEAPTVTGVQGADSDPEMLAHWAKC
EAAGLPSKFRIDPAGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDSVSADRIGTLMHYHYPTTWNHILIDHAVTFRVLPI
SATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDRRIVEENAFGILSPAYEPGPYSELHEGGVIQFVDWYARFIG
PRLAGDGRPALRSVA

Sequences:

>Translated_415_residues
MDARNDMLRLLNGRREGFSLEQPFYTDPDFFKLDMELIWYRDWLFVGHDCELPKPGSFITVQIGDYPVVLVRDQQGRINA
FHNSCRHRGSRVCNTDKGTAAKLVCPYHQWTYELDGRLLFARQMADGFDKSQFGLKPVACESVAGYIFICLAKEPADFAP
MRAMIEPYLKPHRLSEAKIAFESTIVEKGNWKLVWENNRECYHCAGNHPELCKTFPEAPTVTGVQGADSDPEMLAHWAKC
EAAGLPSKFRIDPAGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDSVSADRIGTLMHYHYPTTWNHILIDHAVTFRVLPI
SATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDRRIVEENAFGILSPAYEPGPYSELHEGGVIQFVDWYARFIG
PRLAGDGRPALRSVA
>Mature_415_residues
MDARNDMLRLLNGRREGFSLEQPFYTDPDFFKLDMELIWYRDWLFVGHDCELPKPGSFITVQIGDYPVVLVRDQQGRINA
FHNSCRHRGSRVCNTDKGTAAKLVCPYHQWTYELDGRLLFARQMADGFDKSQFGLKPVACESVAGYIFICLAKEPADFAP
MRAMIEPYLKPHRLSEAKIAFESTIVEKGNWKLVWENNRECYHCAGNHPELCKTFPEAPTVTGVQGADSDPEMLAHWAKC
EAAGLPSKFRIDPAGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDSVSADRIGTLMHYHYPTTWNHILIDHAVTFRVLPI
SATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDRRIVEENAFGILSPAYEPGPYSELHEGGVIQFVDWYARFIG
PRLAGDGRPALRSVA

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=130, Percent_Identity=44.6153846153846, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788103, Length=202, Percent_Identity=32.6732673267327, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 47018; Mature: 47018

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARNDMLRLLNGRREGFSLEQPFYTDPDFFKLDMELIWYRDWLFVGHDCELPKPGSFIT
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCCEEE
VQIGDYPVVLVRDQQGRINAFHNSCRHRGSRVCNTDKGTAAKLVCPYHQWTYELDGRLLF
EEECCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEECCEEEE
ARQMADGFDKSQFGLKPVACESVAGYIFICLAKEPADFAPMRAMIEPYLKPHRLSEAKIA
EEHHHCCCCCHHCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEE
FESTIVEKGNWKLVWENNRECYHCAGNHPELCKTFPEAPTVTGVQGADSDPEMLAHWAKC
HHHHEEECCCEEEEEECCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
EAAGLPSKFRIDPAGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDSVSADRIGTLMHYHY
HHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHEEEEEC
PTTWNHILIDHAVTFRVLPISATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDR
CCCCCEEEEEEEEEEEEEEECCCCHHHHHHHEEEHHHHCCCCCCHHHHHHHHCCCCCHHH
RIVEENAFGILSPAYEPGPYSELHEGGVIQFVDWYARFIGPRLAGDGRPALRSVA
HHHHHCCCEEECCCCCCCCCHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MDARNDMLRLLNGRREGFSLEQPFYTDPDFFKLDMELIWYRDWLFVGHDCELPKPGSFIT
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCCEEE
VQIGDYPVVLVRDQQGRINAFHNSCRHRGSRVCNTDKGTAAKLVCPYHQWTYELDGRLLF
EEECCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEECCEEEE
ARQMADGFDKSQFGLKPVACESVAGYIFICLAKEPADFAPMRAMIEPYLKPHRLSEAKIA
EEHHHCCCCCHHCCCCCHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHEE
FESTIVEKGNWKLVWENNRECYHCAGNHPELCKTFPEAPTVTGVQGADSDPEMLAHWAKC
HHHHEEECCCEEEEEECCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
EAAGLPSKFRIDPAGQYRATRAPLLRDAVSYTMTGKRAVKKNLSDSVSADRIGTLMHYHY
HHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHEEEEEC
PTTWNHILIDHAVTFRVLPISATETAVTTKWLVHKDAVEGVDYDLAELTHVWTETNDQDR
CCCCCEEEEEEEEEEEEEEECCCCHHHHHHHEEEHHHHCCCCCCHHHHHHHHCCCCCHHH
RIVEENAFGILSPAYEPGPYSELHEGGVIQFVDWYARFIGPRLAGDGRPALRSVA
HHHHHCCCEEECCCCCCCCCHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]