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The map label for this gene is mcl2 [H]

Identifier: 13473726

GI number: 13473726

Start: 3503629

End: 3504522

Strand: Direct

Name: mcl2 [H]

Synonym: mlr4422

Alternate gene names: 13473726

Gene position: 3503629-3504522 (Clockwise)

Preceding gene: 13473720

Following gene: 13473727

Centisome position: 49.8

GC content: 65.66

Gene sequence:

>894_bases
ATGACCGAATCCTACCGTCCGCGCCGCTCGGTGCTCTATGTCCCGGCCTCCAACGACAAGGCGCTGGCCAAGATCGCGCT
TCTGTCCTGCGACGCAATCATCATCGATCTCGAGGACGCCGTCGCGCCGGCCGACAAGATTGCCGCCCGCGAAAAGCTGC
CCGCGATCTTCGCCAGCCGCCCGGCTGCCAATCATCTCGGCAACCGCTGCGAGATGGTGGTGCGCGTCAATGCGCTGGCC
AGCGAATGGGGCGCCGACGACCTCTTGGCCGTGGCGAAAGTCGAGCCCGACGGCATCCTGCTGTCCAAGGTCGCCACGCC
GCGCGACATCCTCGAAGTCGGCGACGTACTCGACGACAATTTTGCCTCCGATAGCGTGAAACTCTGGGCGATGATCGAGA
CGCCCAAGGCGCTGCTCAACATCGGCGCCATGGCCGAACTGGGCCGCGATCCAGCCTCTCGGCTCAACTGTTTCGTTGCC
GGAACGAATGATCTGGCCAAGGACACCGGTGTGCTGGTGACGCCGGACCGGCGCTACCTCGTGCCATGGCTGATGCAGAT
GGTGCTGGCCGCGCGCGCCGGCGGCCTCGACATGCTCGACGGCGTTGCCAATGATTTTCGCGACCTCGACGCCTTTGCCC
GTGAGTGCGCGGAGGGGGCCGCCATGGGCTTCGACGGCAAGACGCTGATCCATCCGGCCCAGATCGACGCGGCGAACCGC
GCCTTCGCGCCCTCGCCGGAGGCGGTTGCCGAGGCGCGCCTGATAAAGGACGCCTTTTCACGTGGCGAGAATACTGGCAA
AGGCGTGATCGCGCTGAACGGCAAGATGGTCGAGCGGCTGCATCTGGCACAGGCCGAAAAACTGCTGGCGAAGGCGGCCG
CCATCGGCGCATGA

Upstream 100 bases:

>100_bases
TTTTCCGCAGGCCTGTGCAGTTTTCGTGCAGGGTGCGAGCCAGTTTTCATGCGGGCTTGGACGGCTTGCCTCGACCGAAC
CCAGGCGGCATAGATCGGAT

Downstream 100 bases:

>100_bases
TATAGCCCCATGCTCGCGGGCTATGGGCACTTACGACAGGACTTGGGACATGAAACTCTACCGCTTTCTCACCGGCCCGG
ACGACGCCAGCTTCTGCCAC

Product: citrate lyase beta chain (acyl lyase subunit) (citE)

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTESYRPRRSVLYVPASNDKALAKIALLSCDAIIIDLEDAVAPADKIAAREKLPAIFASRPAANHLGNRCEMVVRVNALA
SEWGADDLLAVAKVEPDGILLSKVATPRDILEVGDVLDDNFASDSVKLWAMIETPKALLNIGAMAELGRDPASRLNCFVA
GTNDLAKDTGVLVTPDRRYLVPWLMQMVLAARAGGLDMLDGVANDFRDLDAFARECAEGAAMGFDGKTLIHPAQIDAANR
AFAPSPEAVAEARLIKDAFSRGENTGKGVIALNGKMVERLHLAQAEKLLAKAAAIGA

Sequences:

>Translated_297_residues
MTESYRPRRSVLYVPASNDKALAKIALLSCDAIIIDLEDAVAPADKIAAREKLPAIFASRPAANHLGNRCEMVVRVNALA
SEWGADDLLAVAKVEPDGILLSKVATPRDILEVGDVLDDNFASDSVKLWAMIETPKALLNIGAMAELGRDPASRLNCFVA
GTNDLAKDTGVLVTPDRRYLVPWLMQMVLAARAGGLDMLDGVANDFRDLDAFARECAEGAAMGFDGKTLIHPAQIDAANR
AFAPSPEAVAEARLIKDAFSRGENTGKGVIALNGKMVERLHLAQAEKLLAKAAAIGA
>Mature_296_residues
TESYRPRRSVLYVPASNDKALAKIALLSCDAIIIDLEDAVAPADKIAAREKLPAIFASRPAANHLGNRCEMVVRVNALAS
EWGADDLLAVAKVEPDGILLSKVATPRDILEVGDVLDDNFASDSVKLWAMIETPKALLNIGAMAELGRDPASRLNCFVAG
TNDLAKDTGVLVTPDRRYLVPWLMQMVLAARAGGLDMLDGVANDFRDLDAFARECAEGAAMGFDGKTLIHPAQIDAANRA
FAPSPEAVAEARLIKDAFSRGENTGKGVIALNGKMVERLHLAQAEKLLAKAAAIGA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=303, Percent_Identity=32.6732673267327, Blast_Score=141, Evalue=7e-34,
Organism=Escherichia coli, GI87081764, Length=297, Percent_Identity=33.6700336700337, Blast_Score=124, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17557458, Length=293, Percent_Identity=31.0580204778157, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31598; Mature: 31466

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESYRPRRSVLYVPASNDKALAKIALLSCDAIIIDLEDAVAPADKIAAREKLPAIFASR
CCCCCCCCCCEEEEECCCCHHHHHHHHEECCEEEEECHHHHCCHHHHHHHHHCCHHHHCC
PAANHLGNRCEMVVRVNALASEWGADDLLAVAKVEPDGILLSKVATPRDILEVGDVLDDN
CCHHHCCCCEEEEEEEHHHHHHCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCC
FASDSVKLWAMIETPKALLNIGAMAELGRDPASRLNCFVAGTNDLAKDTGVLVTPDRRYL
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCHHHCCCEEECCCCCHH
VPWLMQMVLAARAGGLDMLDGVANDFRDLDAFARECAEGAAMGFDGKTLIHPAQIDAANR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCC
AFAPSPEAVAEARLIKDAFSRGENTGKGVIALNGKMVERLHLAQAEKLLAKAAAIGA
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TESYRPRRSVLYVPASNDKALAKIALLSCDAIIIDLEDAVAPADKIAAREKLPAIFASR
CCCCCCCCCEEEEECCCCHHHHHHHHEECCEEEEECHHHHCCHHHHHHHHHCCHHHHCC
PAANHLGNRCEMVVRVNALASEWGADDLLAVAKVEPDGILLSKVATPRDILEVGDVLDDN
CCHHHCCCCEEEEEEEHHHHHHCCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHCCC
FASDSVKLWAMIETPKALLNIGAMAELGRDPASRLNCFVAGTNDLAKDTGVLVTPDRRYL
CCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCHHHCCCEEECCCCCHH
VPWLMQMVLAARAGGLDMLDGVANDFRDLDAFARECAEGAAMGFDGKTLIHPAQIDAANR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCC
AFAPSPEAVAEARLIKDAFSRGENTGKGVIALNGKMVERLHLAQAEKLLAKAAAIGA
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA